################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BbvCI BciVI BclI BfrI BlpI Bpu1102I BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstAPI BstZ17I Bsu36I CelII ClaI CpoI Eam1105I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MfeI MluI MroI MslI MunI NdeI NheI NruI NsiI PacI PflFI PinAI PmeI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1074 GACGTC GACGT^C AflIII 2400 ACACGT A^C[GA][CT]GT Aor51HI 1985 AGCGCT AGC^GCT AvrII 1555 CCTAGG C^CTAGG BamHI 1048 GGATCC G^GATCC BbrPI 2401 CACGTG CAC^GTG BcgI 395 CGATCTCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1428 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 789 AGATCT A^GATCT BlnI 1555 CCTAGG C^CTAGG BsaAI 2401 CACGTG [CT]AC^GT[GA] BsaBI 1519 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 664 GGTCTC GGTCTC(1/5) BsiEI 141 CGGCCG CG[GA][CT]^CG BsmBI 1305 CGTCTC CGTCTC(1/5) Bsp1407I 2256 TGTACA T^GTACA BspMI 805 GCAGGT ACCTGC(4/8) BsrGI 2256 TGTACA T^GTACA BstBI 1851 TTCGAA TT^CGAA BstEII 2193 GGTGACC G^GT[ACGT]ACC BstPI 2193 GGTGACC G^GT[ACGT]ACC BstXI 209 CCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 2460 TTTAAA TTT^AAA DraIII 2215 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2067 GACGGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 141 CGGCCG C^GGCCG EclXI 141 CGGCCG C^GGCCG Eco47III 1985 AGCGCT AGC^GCT Eco52I 141 CGGCCG C^GGCCG EcoO65I 2193 GGTGACC G^GT[ACGT]ACC KspI 1707 CCGCGG CCGC^GG MamI 1519 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NotI 140 GCGGCCGC GC^GGCCGC NspV 1851 TTCGAA TT^CGAA PaeR7I 659 CTCGAG C^TCGAG PmaCI 2401 CACGTG CAC^GTG PmlI 2401 CACGTG CAC^GTG PshAI 880 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 1707 CCGCGG CCGC^GG SexAI 2216 ACCTGGT A^CC[AT]GGT SfuI 1851 TTCGAA TT^CGAA SgrAI 1222 CGCCGGTG C[GA]^CCGG[CT]G SspBI 2256 TGTACA T^GTACA SspI 2329 AATATT AAT^ATT StuI 523 AGGCCT AGG^CCT XhoI 659 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 1495 AAATTC [GA]^AATT[CT] 2276 AAATTC AlwNI 990 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 2181 CAGAAGCTG ApoI 1495 AAATTC [GA]^AATT[CT] 2276 AAATTC AspHI 249 GAGCAC G[AT]GC[AT]^C 1369 GTGCTC AvaI 659 CTCGAG C^[CT]CG[GA]G 1488 CCCGAG BalI 218 TGGCCA TGG^CCA 1228 TGGCCA BbeI 941 GGCGCC GGCGC^C 1202 GGCGCC BsgI 2047 CTGCAC GTGCAG(16/14) 1576 GTGCAG BsiHKAI 249 GAGCAC G[AT]GC[AT]^C 1369 GTGCTC BsmAI 665 GTCTC GTCTC(1/5) 1306 GTCTC BsoBI 659 CTCGAG C^[CT]CG[GA]G 1488 CCCGAG BssSI 441 CACGAG CACGAG(-5/-1) 423 CTCGTG HgaI 880 GACGC GACGC(5/10) 2012 GCGTC KasI 941 GGCGCC G^GCGCC 1202 GGCGCC MluNI 218 TGGCCA TGG^CCA 1228 TGGCCA MscI 218 TGGCCA TGG^CCA 1228 TGGCCA MseI 2370 TTAA T^TAA 2461 TTAA NaeI 1239 GCCGGC GCC^GGC 1603 GCCGGC NarI 941 GGCGCC GG^CGCC 1202 GGCGCC NgoMIV 1239 GCCGGC G^CCGGC 1603 GCCGGC PpuMI 1054 AGGACCC [GA]G^G[AT]CC[CT] 1248 GGGACCT PstI 514 CTGCAG CTGCA^G 1423 CTGCAG SfoI 941 GGCGCC GGC^GCC 1202 GGCGCC SphI 226 GCATGC GCATG^C 1349 GCATGC Tru9I 2370 TTAA T^TAA 2461 TTAA XcmI 2079 CCATCCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2225 CCATGGGCAGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII