##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BbvCI BciVI
BclI BfrI BlpI Bpu1102I BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BssHII Bst1107I
BstAPI BstZ17I Bsu36I CelII ClaI
CpoI Eam1105I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MfeI MluI MroI MslI MunI
NdeI NheI NruI NsiI PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SfiI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1074 GACGTC GACGT^C
AflIII 2400 ACACGT A^C[GA][CT]GT
Aor51HI 1985 AGCGCT AGC^GCT
AvrII 1555 CCTAGG C^CTAGG
BamHI 1048 GGATCC G^GATCC
BbrPI 2401 CACGTG CAC^GTG
BcgI 395 CGATCTCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1428 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 789 AGATCT A^GATCT
BlnI 1555 CCTAGG C^CTAGG
BsaAI 2401 CACGTG [CT]AC^GT[GA]
BsaBI 1519 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 664 GGTCTC GGTCTC(1/5)
BsiEI 141 CGGCCG CG[GA][CT]^CG
BsmBI 1305 CGTCTC CGTCTC(1/5)
Bsp1407I 2256 TGTACA T^GTACA
BspMI 805 GCAGGT ACCTGC(4/8)
BsrGI 2256 TGTACA T^GTACA
BstBI 1851 TTCGAA TT^CGAA
BstEII 2193 GGTGACC G^GT[ACGT]ACC
BstPI 2193 GGTGACC G^GT[ACGT]ACC
BstXI 209 CCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2460 TTTAAA TTT^AAA
DraIII 2215 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2067 GACGGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 141 CGGCCG C^GGCCG
EclXI 141 CGGCCG C^GGCCG
Eco47III 1985 AGCGCT AGC^GCT
Eco52I 141 CGGCCG C^GGCCG
EcoO65I 2193 GGTGACC G^GT[ACGT]ACC
KspI 1707 CCGCGG CCGC^GG
MamI 1519 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 140 GCGGCCGC GC^GGCCGC
NspV 1851 TTCGAA TT^CGAA
PaeR7I 659 CTCGAG C^TCGAG
PmaCI 2401 CACGTG CAC^GTG
PmlI 2401 CACGTG CAC^GTG
PshAI 880 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1707 CCGCGG CCGC^GG
SexAI 2216 ACCTGGT A^CC[AT]GGT
SfuI 1851 TTCGAA TT^CGAA
SgrAI 1222 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 2256 TGTACA T^GTACA
SspI 2329 AATATT AAT^ATT
StuI 523 AGGCCT AGG^CCT
XhoI 659 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1495 AAATTC [GA]^AATT[CT]
2276 AAATTC
AlwNI 990 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2181 CAGAAGCTG
ApoI 1495 AAATTC [GA]^AATT[CT]
2276 AAATTC
AspHI 249 GAGCAC G[AT]GC[AT]^C
1369 GTGCTC
AvaI 659 CTCGAG C^[CT]CG[GA]G
1488 CCCGAG
BalI 218 TGGCCA TGG^CCA
1228 TGGCCA
BbeI 941 GGCGCC GGCGC^C
1202 GGCGCC
BsgI 2047 CTGCAC GTGCAG(16/14)
1576 GTGCAG
BsiHKAI 249 GAGCAC G[AT]GC[AT]^C
1369 GTGCTC
BsmAI 665 GTCTC GTCTC(1/5)
1306 GTCTC
BsoBI 659 CTCGAG C^[CT]CG[GA]G
1488 CCCGAG
BssSI 441 CACGAG CACGAG(-5/-1)
423 CTCGTG
HgaI 880 GACGC GACGC(5/10)
2012 GCGTC
KasI 941 GGCGCC G^GCGCC
1202 GGCGCC
MluNI 218 TGGCCA TGG^CCA
1228 TGGCCA
MscI 218 TGGCCA TGG^CCA
1228 TGGCCA
MseI 2370 TTAA T^TAA
2461 TTAA
NaeI 1239 GCCGGC GCC^GGC
1603 GCCGGC
NarI 941 GGCGCC GG^CGCC
1202 GGCGCC
NgoMIV 1239 GCCGGC G^CCGGC
1603 GCCGGC
PpuMI 1054 AGGACCC [GA]G^G[AT]CC[CT]
1248 GGGACCT
PstI 514 CTGCAG CTGCA^G
1423 CTGCAG
SfoI 941 GGCGCC GGC^GCC
1202 GGCGCC
SphI 226 GCATGC GCATG^C
1349 GCATGC
Tru9I 2370 TTAA T^TAA
2461 TTAA
XcmI 2079 CCATCCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2225 CCATGGGCAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII