##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HindIII HpaI KpnI KspI MfeI
MluI MluNI MroI MscI MspA1I
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmaI SmlI
SnaBI Sse8387I SspBI StuI SwaI
Tsp45I Tth111I Van91I XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 1 GGCGCC G[GA]^CG[CT]C
AfaI 447 GTAC GT^AC
AflIII 1246 ACATGT A^C[GA][CT]GT
Alw44I 1021 GTGCAC G^TGCAC
AlwNI 462 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 542 CCTGAGG CC^T[ACGT]AGG
ApaI 585 GGGCCC GGGCC^C
ApaLI 1021 GTGCAC G^TGCAC
AvaII 547 GGTCC G^G[AT]CC
AvrII 934 CCTAGG C^CTAGG
BbeI 1 GGCGCC GGCGC^C
BglI 887 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 934 CCTAGG C^CTAGG
BpmI 889 CTGGAG CTGGAG(16/14)
BsaBI 648 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1 GGCGCC G[GA]^CG[CT]C
BseRI 1122 GAGGAG GAGGAG(10/8)
BsmBI 439 GAGACG CGTCTC(1/5)
BsmFI 961 GTCCC GGGAC(10/14)
BspLU11I 1246 ACATGT A^CATGT
BsrDI 84 CATTGC GCAATG(2/0)
BssHII 10 GCGCGC G^CGCGC
BstYI 408 AGATCC [GA]^GATC[CT]
Bsu36I 542 CCTGAGG CC^T[ACGT]AGG
EaeI 519 CGGCCA [CT]^GGCC[GA]
Eco81I 542 CCTGAGG CC^T[ACGT]AGG
EcoT14I 934 CCTAGG C^C[AT][AT]GG
FauI 14 GCGGG CCCGC(4/6)
HaeII 1 GGCGCC [GA]GCGC^[CT]
Hin1I 1 GGCGCC G[GA]^CG[CT]C
HphI 79 TCACC GGTGA(8/7)
KasI 1 GGCGCC G^GCGCC
MamI 648 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 408 AGATCC [GA]^GATC[CT]
NarI 1 GGCGCC GG^CGCC
PpuMI 546 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 954 GACAGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 468 CTGCAG CTGCA^G
RsaI 447 GTAC GT^AC
SacI 974 GAGCTC GAGCT^C
SfcI 468 CTGCAG C^T[GA][CT]AG
SfoI 1 GGCGCC GGC^GCC
SpeI 731 ACTAGT A^CTAGT
SphI 1325 GCATGC GCATG^C
StyI 934 CCTAGG C^C[AT][AT]GG
XbaI 984 TCTAGA T^CTAGA
XhoII 408 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 145 GTAGAC GT^[AC][GT]AC
857 GTCTAC
AlwI 382 GATCC GGATC(4/5)
409 GATCC
AspHI 974 GAGCTC G[AT]GC[AT]^C
1021 GTGCAC
BanII 585 GGGCCC G[GA]GC[CT]^C
974 GAGCTC
BcnI 164 CCGGG CC^[GC]GG
245 CCCGG
BsiHKAI 974 GAGCTC G[AT]GC[AT]^C
1021 GTGCAC
BsmAI 439 GAGAC GTCTC(1/5)
840 GAGAC
BsmI 224 GCATTC GAATGC(1/-1)
721 GCATTC
DraI 1284 TTTAAA TTT^AAA
1295 TTTAAA
HapII 164 CCGG C^CGG
246 CCGG
HincII 611 GTTGAC GT[CT]^[GA]AC
821 GTCAAC
HindII 611 GTTGAC GT[CT]^[GA]AC
821 GTCAAC
HpaII 164 CCGG C^CGG
246 CCGG
MaeII 239 ACGT A^CGT
577 ACGT
MslI 225 CATTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1194 CACTTGTATG
MspI 164 CCGG C^CGG
246 CCGG
MwoI 25 GCGGAACAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
887 GCCTGGAGGGC
NciI 164 CCGGG CC^[GC]GG
245 CCCGG
NspI 1246 ACATGT [GA]CATG^[CT]
1325 GCATGC
PleI 427 GACTC GAGTC(4/5)
855 GAGTC
SspI 1334 AATATT AAT^ATT
1427 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI BanI BbvI
BfaI BmyI BsaJI BsiYI BslI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MvaI MvnI NdeII NlaIII
NlaIV RcaI Sau3AI Sau96I ScrFI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I