##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI Aor51HI ApaI AscI AspEI
AspI AviII AvrII BalI BamHI
BcgI BciVI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
CelII ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoT22I FseI FspI HincII HindII
HindIII HpaI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NcoI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI TfiI
Tth111I XbaI XcmI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcsI 1828 AAATTT [GA]^AATT[CT]
AflII 1527 CTTAAG C^TTAAG
AocI 624 CCTCAGG CC^T[ACGT]AGG
ApoI 1828 AAATTT [GA]^AATT[CT]
AseI 1896 ATTAAT AT^TAAT
AsnI 1896 ATTAAT AT^TAAT
AvaI 807 CTCGAG C^[CT]CG[GA]G
AvaII 181 GGTCC G^G[AT]CC
BanII 1781 GAGCCC G[GA]GC[CT]^C
BbeI 109 GGCGCC GGCGC^C
BbrPI 1660 CACGTG CAC^GTG
BbsI 892 GAAGAC GAAGAC(2/6)
BbvCI 824 GCTGAGG CCTCAGC(-5/-2)
BclI 1506 TGATCA T^GATCA
BcnI 88 CCGGG CC^[GC]GG
BfrI 1527 CTTAAG C^TTAAG
BpuAI 892 GAAGAC GAAGAC(2/6)
BsaI 859 GAGACC GGTCTC(1/5)
BsaWI 155 TCCGGT [AT]^CCGG[AT]
BseRI 314 GAGGAG GAGGAG(10/8)
BsoBI 807 CTCGAG C^[CT]CG[GA]G
BspLU11I 513 ACATGT A^CATGT
BspMI 933 ACCTGC ACCTGC(4/8)
BsrDI 1377 CATTGC GCAATG(2/0)
BsrFI 106 GCCGGC [GA]^CCGG[CT]
BstAPI 762 GCATTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 367 CCACCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 624 CCTCAGG CC^T[ACGT]AGG
Cfr10I 106 GCCGGC [GA]^CCGG[CT]
DsaI 441 CCGTGG C^C[GA][CT]GG
Eco81I 624 CCTCAGG CC^T[ACGT]AGG
EcoRV 212 GATATC GAT^ATC
EcoT14I 1446 CCAAGG C^C[AT][AT]GG
FbaI 1506 TGATCA T^GATCA
HaeII 109 GGCGCC [GA]GCGC^[CT]
HgaI 362 GACGC GACGC(5/10)
KasI 109 GGCGCC G^GCGCC
NaeI 106 GCCGGC GCC^GGC
NarI 109 GGCGCC GG^CGCC
NciI 88 CCGGG CC^[GC]GG
NgoMIV 106 GCCGGC G^CCGGC
NspI 513 ACATGT [GA]CATG^[CT]
PaeR7I 807 CTCGAG C^TCGAG
PflMI 436 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1660 CACGTG CAC^GTG
PmlI 1660 CACGTG CAC^GTG
PshBI 1896 ATTAAT AT^TAAT
SfoI 109 GGCGCC GGC^GCC
StyI 1446 CCAAGG C^C[AT][AT]GG
Van91I 436 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 807 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 84 GGTACC G^GTACC
221 GGTACC
AcyI 109 GGCGCC G[GA]^CG[CT]C
362 GACGCC
AflIII 513 ACATGT A^C[GA][CT]GT
1661 ACGTGT
Alw44I 839 GTGCAC G^TGCAC
1592 GTGCAC
AlwNI 396 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
644 CAGAAACTG
ApaLI 839 GTGCAC G^TGCAC
1592 GTGCAC
Asp718I 84 GGTACC G^GTACC
221 GGTACC
BsaAI 842 CACGTA [CT]AC^GT[GA]
1660 CACGTG
BsaHI 109 GGCGCC G[GA]^CG[CT]C
362 GACGCC
BsgI 31 CTGCAC GTGCAG(16/14)
489 CTGCAC
BsmAI 859 GAGAC GTCTC(1/5)
1124 GTCTC
BsmI 527 GCATTC GAATGC(1/-1)
661 GCATTC
Bsp1407I 612 TGTACA T^GTACA
1050 TGTACA
BsrGI 612 TGTACA T^GTACA
1050 TGTACA
BsrI 816 CCAGT ACTGG(1/-1)
1580 CCAGT
EarI 311 GAAGAG CTCTTC(1/4)
356 GAAGAG
EcoNI 948 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1198 CCTTAGTGAGG
Hin1I 109 GGCGCC G[GA]^CG[CT]C
362 GACGCC
HinfI 142 GAGTC G^A[ACGT]TC
1500 GAGTC
HphI 856 GGTGA GGTGA(8/7)
195 TCACC
KpnI 84 GGTACC GGTAC^C
221 GGTACC
Ksp632I 311 GAAGAG CTCTTC(1/4)
356 GAAGAG
NdeI 1046 CATATG CA^TATG
1056 CATATG
PleI 142 GAGTC GAGTC(4/5)
1500 GAGTC
SspBI 612 TGTACA T^GTACA
1050 TGTACA
SspI 1335 AATATT AAT^ATT
1351 AATATT
Tsp45I 194 GTCAC ^GT[GC]AC
699 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
Asp700I AspHI BanI BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I XmnI