##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbrPI
BbsI BbvCI BcgI BfrI BglII
BlnI BmyI BpuAI BsaAI BsaBI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI HphI KpnI KspI
MaeII MamI MflI MluI MluNI
MroI MscI MvnI NaeI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 1366 AAATTT [GA]^AATT[CT]
AcyI 1007 GGCGCC G[GA]^CG[CT]C
AlwI 514 GGATC GGATC(4/5)
ApoI 1366 AAATTT [GA]^AATT[CT]
BbeI 1007 GGCGCC GGCGC^C
BclI 1497 TGATCA T^GATCA
BcnI 700 CCGGG CC^[GC]GG
BglI 923 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1007 GGCGCC G[GA]^CG[CT]C
BsaI 388 GAGACC GGTCTC(1/5)
BsmFI 1515 GGGAC GGGAC(10/14)
BspHI 206 TCATGA T^CATGA
BspMI 243 GCAGGT ACCTGC(4/8)
BsrBI 1015 CCGCTC CCGCTC(-3/-3)
BssSI 1591 CTCGTG CACGAG(-5/-1)
BstAPI 729 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 1008 GCGC GCG^C
DraIII 1525 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 837 CCACGG C^C[GA][CT]GG
EcoT14I 534 CCTTGG C^C[AT][AT]GG
FauI 1011 CCCGC CCCGC(4/6)
FbaI 1497 TGATCA T^GATCA
HaeII 1007 GGCGCC [GA]GCGC^[CT]
HapII 700 CCGG C^CGG
HhaI 1008 GCGC GCG^C
Hin1I 1007 GGCGCC G[GA]^CG[CT]C
HinP1I 1008 GCGC G^CGC
HpaII 700 CCGG C^CGG
KasI 1007 GGCGCC G^GCGCC
MfeI 1446 CAATTG C^AATTG
MspI 700 CCGG C^CGG
MunI 1446 CAATTG C^AATTG
NarI 1007 GGCGCC GG^CGCC
NciI 700 CCGGG CC^[GC]GG
NheI 576 GCTAGC G^CTAGC
NspI 302 GCATGT [GA]CATG^[CT]
PleI 1475 GAGTC GAGTC(4/5)
RcaI 206 TCATGA T^CATGA
SapI 1017 GCTCTTC GCTCTTC(1/4)
SfoI 1007 GGCGCC GGC^GCC
StyI 534 CCTTGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 2 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
730 CAGCCACTG
BanI 741 GGCACC G^G[CT][GA]CC
1007 GGCGCC
BciVI 1459 GGATAC GTATCC(6/5)
591 GTATCC
BfaI 53 CTAG C^TAG
577 CTAG
BlpI 887 GCTGAGC GC^T[ACGT]AGC
896 GCTGAGC
Bpu1102I 887 GCTGAGC GC^T[ACGT]AGC
896 GCTGAGC
BsgI 105 GTGCAG GTGCAG(16/14)
241 GTGCAG
BsmBI 1551 GAGACG CGTCTC(1/5)
1560 GAGACG
BstF5I 290 GGATG GGATG(2/0)
538 GGATG
CelII 887 GCTGAGC GC^T[ACGT]AGC
896 GCTGAGC
EaeI 346 TGGCCG [CT]^GGCC[GA]
404 TGGCCG
FokI 290 GGATG GGATG(9/13)
538 GGATG
HgaI 1562 GACGC GACGC(5/10)
4 GCGTC
MaeI 53 CTAG C^TAG
577 CTAG
MspA1I 724 CCGCTG C[AC]G^C[GT]G
745 CCGCTG
PpuMI 37 AGGACCT [GA]G^G[AT]CC[CT]
245 AGGTCCT
StuI 185 AGGCCT AGG^CCT
1410 AGGCCT
TaqI 124 TCGA T^CGA
1277 TCGA
TthHB8I 124 TCGA T^CGA
1277 TCGA
XcmI 147 CCAGTGAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
392 CCATTGCATCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsrDI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI HaeIII HinfI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI