################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII BalI BbrPI BbvCI BciVI BfrI BglI BglII BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 186 CGCG CG^CG AcyI 504 GGCGCC G[GA]^CG[CT]C Aor51HI 165 AGCGCT AGC^GCT ApaI 174 GGGCCC GGGCC^C AvaII 8 GGTCC G^G[AT]CC AvrII 499 CCTAGG C^CTAGG BamHI 410 GGATCC G^GATCC BanII 174 GGGCCC G[GA]GC[CT]^C BbeI 504 GGCGCC GGCGC^C BbsI 1470 GTCTTC GAAGAC(2/6) BcgI 282 CGACAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 871 TGATCA T^GATCA BcnI 77 CCGGG CC^[GC]GG BlnI 499 CCTAGG C^CTAGG BpmI 899 CTCCAG CTGGAG(16/14) BpuAI 1470 GTCTTC GAAGAC(2/6) BsaAI 707 CACGTA [CT]AC^GT[GA] BsaHI 504 GGCGCC G[GA]^CG[CT]C BsaI 1230 GGTCTC GGTCTC(1/5) BsmFI 366 GGGAC GGGAC(10/14) BspHI 1555 TCATGA T^CATGA BsrBI 111 GAGCGG CCGCTC(-3/-3) BsrDI 148 CATTGC GCAATG(2/0) BstEII 608 GGTCACC G^GT[ACGT]ACC BstPI 608 GGTCACC G^GT[ACGT]ACC BstUI 186 CGCG CG^CG DsaI 1189 CCATGG C^C[GA][CT]GG Eco47III 165 AGCGCT AGC^GCT EcoNI 907 CCTGGGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 608 GGTCACC G^GT[ACGT]ACC EcoRI 688 GAATTC G^AATTC FbaI 871 TGATCA T^GATCA HapII 77 CCGG C^CGG HgaI 2 GACGC GACGC(5/10) Hin1I 504 GGCGCC G[GA]^CG[CT]C HpaII 77 CCGG C^CGG KasI 504 GGCGCC G^GCGCC MaeII 708 ACGT A^CGT MspI 77 CCGG C^CGG MvnI 186 CGCG CG^CG NarI 504 GGCGCC GG^CGCC NciI 77 CCGGG CC^[GC]GG NcoI 1189 CCATGG C^CATGG PpuMI 7 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 2 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 219 CTGCAG CTGCA^G RcaI 1555 TCATGA T^CATGA SapI 1402 GCTCTTC GCTCTTC(1/4) SfaNI 1399 GATGC GCATC(5/9) SfoI 504 GGCGCC GGC^GCC SspI 807 AATATT AAT^ATT XcmI 595 CCACTCCCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 688 GAATTC [GA]^AATT[CT] 888 AAATTC AfaI 989 GTAC GT^AC 1528 GTAC AocI 1323 CCTGAGG CC^T[ACGT]AGG 1376 CCTGAGG ApoI 688 GAATTC [GA]^AATT[CT] 888 AAATTC Asp700I 511 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1065 GAATATCTTC AvaI 125 CTCGGG C^[CT]CG[GA]G 303 CTCGGG BlpI 161 GCTGAGC GC^T[ACGT]AGC 272 GCTAAGC BmyI 174 GGGCCC G[AGT]GC[ACT]^C 856 GTGCCC Bpu1102I 161 GCTGAGC GC^T[ACGT]AGC 272 GCTAAGC BsgI 243 GTGCAG GTGCAG(16/14) 360 GTGCAG BsoBI 125 CTCGGG C^[CT]CG[GA]G 303 CTCGGG Bsp1286I 174 GGGCCC G[AGT]GC[ACT]^C 856 GTGCCC BspMI 1648 ACCTGC ACCTGC(4/8) 5 GCAGGT BsrI 227 ACTGG ACTGG(1/-1) 440 ACTGG BstF5I 732 CATCC GGATG(2/0) 787 GGATG BstYI 410 GGATCC [GA]^GATC[CT] 950 AGATCC Bsu36I 1323 CCTGAGG CC^T[ACGT]AGG 1376 CCTGAGG CelII 161 GCTGAGC GC^T[ACGT]AGC 272 GCTAAGC Eco81I 1323 CCTGAGG CC^T[ACGT]AGG 1376 CCTGAGG FokI 732 CATCC GGATG(9/13) 787 GGATG MflI 410 GGATCC [GA]^GATC[CT] 950 AGATCC NspI 382 GCATGT [GA]CATG^[CT] 717 GCATGT PleI 482 GACTC GAGTC(4/5) 1217 GACTC RsaI 989 GTAC GT^AC 1528 GTAC SfcI 219 CTGCAG C^T[GA][CT]AG 527 CTACAG SmlI 139 CTCAAG C^T[CT][GA]AG 539 CTCAAG XhoII 410 GGATCC [GA]^GATC[CT] 950 AGATCC XmnI 511 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1065 GAATATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AlwI AlwNI BanI BbvI BfaI BsaJI BseRI BsiYI BslI BsmAI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PflMI Sau3AI Sau96I ScrFI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I