##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII BalI
BbrPI BbvCI BciVI BfrI BglI
BglII BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 186 CGCG CG^CG
AcyI 504 GGCGCC G[GA]^CG[CT]C
Aor51HI 165 AGCGCT AGC^GCT
ApaI 174 GGGCCC GGGCC^C
AvaII 8 GGTCC G^G[AT]CC
AvrII 499 CCTAGG C^CTAGG
BamHI 410 GGATCC G^GATCC
BanII 174 GGGCCC G[GA]GC[CT]^C
BbeI 504 GGCGCC GGCGC^C
BbsI 1470 GTCTTC GAAGAC(2/6)
BcgI 282 CGACAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 871 TGATCA T^GATCA
BcnI 77 CCGGG CC^[GC]GG
BlnI 499 CCTAGG C^CTAGG
BpmI 899 CTCCAG CTGGAG(16/14)
BpuAI 1470 GTCTTC GAAGAC(2/6)
BsaAI 707 CACGTA [CT]AC^GT[GA]
BsaHI 504 GGCGCC G[GA]^CG[CT]C
BsaI 1230 GGTCTC GGTCTC(1/5)
BsmFI 366 GGGAC GGGAC(10/14)
BspHI 1555 TCATGA T^CATGA
BsrBI 111 GAGCGG CCGCTC(-3/-3)
BsrDI 148 CATTGC GCAATG(2/0)
BstEII 608 GGTCACC G^GT[ACGT]ACC
BstPI 608 GGTCACC G^GT[ACGT]ACC
BstUI 186 CGCG CG^CG
DsaI 1189 CCATGG C^C[GA][CT]GG
Eco47III 165 AGCGCT AGC^GCT
EcoNI 907 CCTGGGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 608 GGTCACC G^GT[ACGT]ACC
EcoRI 688 GAATTC G^AATTC
FbaI 871 TGATCA T^GATCA
HapII 77 CCGG C^CGG
HgaI 2 GACGC GACGC(5/10)
Hin1I 504 GGCGCC G[GA]^CG[CT]C
HpaII 77 CCGG C^CGG
KasI 504 GGCGCC G^GCGCC
MaeII 708 ACGT A^CGT
MspI 77 CCGG C^CGG
MvnI 186 CGCG CG^CG
NarI 504 GGCGCC GG^CGCC
NciI 77 CCGGG CC^[GC]GG
NcoI 1189 CCATGG C^CATGG
PpuMI 7 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 219 CTGCAG CTGCA^G
RcaI 1555 TCATGA T^CATGA
SapI 1402 GCTCTTC GCTCTTC(1/4)
SfaNI 1399 GATGC GCATC(5/9)
SfoI 504 GGCGCC GGC^GCC
SspI 807 AATATT AAT^ATT
XcmI 595 CCACTCCCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 688 GAATTC [GA]^AATT[CT]
888 AAATTC
AfaI 989 GTAC GT^AC
1528 GTAC
AocI 1323 CCTGAGG CC^T[ACGT]AGG
1376 CCTGAGG
ApoI 688 GAATTC [GA]^AATT[CT]
888 AAATTC
Asp700I 511 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1065 GAATATCTTC
AvaI 125 CTCGGG C^[CT]CG[GA]G
303 CTCGGG
BlpI 161 GCTGAGC GC^T[ACGT]AGC
272 GCTAAGC
BmyI 174 GGGCCC G[AGT]GC[ACT]^C
856 GTGCCC
Bpu1102I 161 GCTGAGC GC^T[ACGT]AGC
272 GCTAAGC
BsgI 243 GTGCAG GTGCAG(16/14)
360 GTGCAG
BsoBI 125 CTCGGG C^[CT]CG[GA]G
303 CTCGGG
Bsp1286I 174 GGGCCC G[AGT]GC[ACT]^C
856 GTGCCC
BspMI 1648 ACCTGC ACCTGC(4/8)
5 GCAGGT
BsrI 227 ACTGG ACTGG(1/-1)
440 ACTGG
BstF5I 732 CATCC GGATG(2/0)
787 GGATG
BstYI 410 GGATCC [GA]^GATC[CT]
950 AGATCC
Bsu36I 1323 CCTGAGG CC^T[ACGT]AGG
1376 CCTGAGG
CelII 161 GCTGAGC GC^T[ACGT]AGC
272 GCTAAGC
Eco81I 1323 CCTGAGG CC^T[ACGT]AGG
1376 CCTGAGG
FokI 732 CATCC GGATG(9/13)
787 GGATG
MflI 410 GGATCC [GA]^GATC[CT]
950 AGATCC
NspI 382 GCATGT [GA]CATG^[CT]
717 GCATGT
PleI 482 GACTC GAGTC(4/5)
1217 GACTC
RsaI 989 GTAC GT^AC
1528 GTAC
SfcI 219 CTGCAG C^T[GA][CT]AG
527 CTACAG
SmlI 139 CTCAAG C^T[CT][GA]AG
539 CTCAAG
XhoII 410 GGATCC [GA]^GATC[CT]
950 AGATCC
XmnI 511 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1065 GAATATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AlwNI BanI
BbvI BfaI BsaJI BseRI BsiYI
BslI BsmAI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PflMI Sau3AI Sau96I ScrFI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I