##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT14I
FseI FspI HgaI HindIII HpaI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
StyI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccII 23 CGCG CG^CG
AcyI 104 GGCGCC G[GA]^CG[CT]C
Alw44I 1655 GTGCAC G^TGCAC
AlwNI 1842 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 425 CCTAAGG CC^T[ACGT]AGG
ApaLI 1655 GTGCAC G^TGCAC
AspHI 1655 GTGCAC G[AT]GC[AT]^C
AvaI 645 CTCGGG C^[CT]CG[GA]G
BalI 959 TGGCCA TGG^CCA
BanI 104 GGCGCC G^G[CT][GA]CC
BanII 55 GGGCTC G[GA]GC[CT]^C
BbeI 104 GGCGCC GGCGC^C
BcgI 480 GCATTTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1439 TGATCA T^GATCA
BsaHI 104 GGCGCC G[GA]^CG[CT]C
BsaI 1315 GGTCTC GGTCTC(1/5)
BsaJI 15 CCCCGG C^C[ACGT][ACGT]GG
BseRI 207 GAGGAG GAGGAG(10/8)
BsiHKAI 1655 GTGCAC G[AT]GC[AT]^C
BsmBI 131 GAGACG CGTCTC(1/5)
BsoBI 645 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1875 TGTACA T^GTACA
BspHI 1963 TCATGA T^CATGA
BsrDI 1680 CATTGC GCAATG(2/0)
BsrGI 1875 TGTACA T^GTACA
BstBI 1619 TTCGAA TT^CGAA
BstUI 23 CGCG CG^CG
BstYI 606 GGATCT [GA]^GATC[CT]
Bsu36I 425 CCTAAGG CC^T[ACGT]AGG
Eco81I 425 CCTAAGG CC^T[ACGT]AGG
EcoRV 2064 GATATC GAT^ATC
EcoT22I 1231 ATGCAT ATGCA^T
FauI 149 CCCGC CCCGC(4/6)
FbaI 1439 TGATCA T^GATCA
Hin1I 104 GGCGCC G[GA]^CG[CT]C
HincII 1673 GTTGAC GT[CT]^[GA]AC
HindII 1673 GTTGAC GT[CT]^[GA]AC
KasI 104 GGCGCC G^GCGCC
MflI 606 GGATCT [GA]^GATC[CT]
MluNI 959 TGGCCA TGG^CCA
MscI 959 TGGCCA TGG^CCA
MspA1I 1886 CAGCTG C[AC]G^C[GT]G
MvnI 23 CGCG CG^CG
MwoI 98 GCAAAAGGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 104 GGCGCC GG^CGCC
NsiI 1231 ATGCAT ATGCA^T
NspV 1619 TTCGAA TT^CGAA
PstI 297 CTGCAG CTGCA^G
PvuII 1886 CAGCTG CAG^CTG
RcaI 1963 TCATGA T^CATGA
SapI 57 GCTCTTC GCTCTTC(1/4)
ScaI 1939 AGTACT AGT^ACT
SfoI 104 GGCGCC GGC^GCC
SfuI 1619 TTCGAA TT^CGAA
SspBI 1875 TGTACA T^GTACA
TfiI 45 GATTC G^A[AT]TC
XhoII 606 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 32 CTTAAG C^TTAAG
842 CTTAAG
BbsI 837 GAAGAC GAAGAC(2/6)
967 GTCTTC
BciVI 1075 GGATAC GTATCC(6/5)
2006 GTATCC
BcnI 16 CCCGG CC^[GC]GG
156 CCGGG
BfrI 32 CTTAAG C^TTAAG
842 CTTAAG
BmyI 55 GGGCTC G[AGT]GC[ACT]^C
1655 GTGCAC
BpmI 282 CTCCAG CTGGAG(16/14)
1174 CTGGAG
BpuAI 837 GAAGAC GAAGAC(2/6)
967 GTCTTC
BsgI 1741 CTGCAC GTGCAG(16/14)
1839 GTGCAG
Bsp1286I 55 GGGCTC G[AGT]GC[ACT]^C
1655 GTGCAC
BspMI 698 ACCTGC ACCTGC(4/8)
299 GCAGGT
BsrI 1752 ACTGG ACTGG(1/-1)
305 CCAGT
BstXI 336 CCACAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2010 CCATTGAAATGG
Cac8I 331 GCAAGC GC[ACGT]^[ACGT]GC
514 GCCTGC
CfoI 105 GCGC GCG^C
236 GCGC
EaeI 175 TGGCCG [CT]^GGCC[GA]
959 TGGCCA
EarI 58 CTCTTC CTCTTC(1/4)
1865 CTCTTC
HaeII 104 GGCGCC [GA]GCGC^[CT]
235 GGCGCT
HhaI 105 GCGC GCG^C
236 GCGC
HinP1I 105 GCGC G^CGC
236 GCGC
HphI 2076 GGTGA GGTGA(8/7)
1869 TCACC
Ksp632I 58 CTCTTC CTCTTC(1/4)
1865 CTCTTC
NciI 16 CCCGG CC^[GC]GG
156 CCGGG
PleI 655 GACTC GAGTC(4/5)
916 GACTC
SmlI 32 CTTAAG C^T[CT][GA]AG
842 CTTAAG
SspI 461 AATATT AAT^ATT
1631 AATATT
SwaI 1339 ATTTAAAT ATTT^AAAT
2050 ATTTAAAT
TaqI 489 TCGA T^CGA
1620 TCGA
TthHB8I 489 TCGA T^CGA
1620 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaII BbvI
BfaI BsiYI BslI BsmAI BsmFI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraI DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI NdeII NlaIII NlaIV NspI
PpuMI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI Tru9I TseI
Tsp45I Tsp509I TspRI