##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AcyI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI BbeI BbrPI BcgI BfrI
BglI BglII BsaAI BsaBI BsaHI
BsgI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FauI FseI Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MluI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccII 462 CGCG CG^CG
AccIII 206 TCCGGA T^CCGGA
AclI 651 AACGTT AA^CGTT
AcsI 1881 GAATTT [GA]^AATT[CT]
AflIII 1062 ACATGT A^C[GA][CT]GT
AhdI 448 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1461 GGGCCC GGGCC^C
ApoI 1881 GAATTT [GA]^AATT[CT]
AspEI 448 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 372 TGCGCA TGC^GCA
AvrII 1396 CCTAGG C^CTAGG
BalI 1183 TGGCCA TGG^CCA
BciVI 2110 GTATCC GTATCC(6/5)
BclI 1476 TGATCA T^GATCA
BlnI 1396 CCTAGG C^CTAGG
BlpI 101 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 101 GCTGAGC GC^T[ACGT]AGC
BseAI 206 TCCGGA T^CCGGA
BspEI 206 TCCGGA T^CCGGA
BspLU11I 1062 ACATGT A^CATGT
BsrBI 901 CCGCTC CCGCTC(-3/-3)
BsrDI 1819 CATTGC GCAATG(2/0)
BstEII 1745 GGTCACC G^GT[ACGT]ACC
BstPI 1745 GGTCACC G^GT[ACGT]ACC
BstUI 462 CGCG CG^CG
BstXI 1592 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 101 GCTGAGC GC^T[ACGT]AGC
DrdI 183 GACACCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 448 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1031 CTCTTC CTCTTC(1/4)
EcoNI 319 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1745 GGTCACC G^GT[ACGT]ACC
FbaI 1476 TGATCA T^GATCA
FspI 372 TGCGCA TGC^GCA
HaeII 234 GGCGCT [GA]GCGC^[CT]
HgaI 538 GCGTC GACGC(5/10)
HindIII 1146 AAGCTT A^AGCTT
Ksp632I 1031 CTCTTC CTCTTC(1/4)
MfeI 157 CAATTG C^AATTG
MluNI 1183 TGGCCA TGG^CCA
MroI 206 TCCGGA T^CCGGA
MscI 1183 TGGCCA TGG^CCA
MslI 166 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 157 CAATTG C^AATTG
MvnI 462 CGCG CG^CG
NcoI 63 CCATGG C^CATGG
NheI 2172 GCTAGC G^CTAGC
NspI 1062 ACATGT [GA]CATG^[CT]
PflMI 1465 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 787 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 651 AACGTT AA^CGTT
PvuI 1048 CGATCG CGAT^CG
SapI 1030 GCTCTTC GCTCTTC(1/4)
ScaI 48 AGTACT AGT^ACT
SfcI 1709 CTACAG C^T[GA][CT]AG
SmlI 2206 CTCAAG C^T[CT][GA]AG
StuI 670 AGGCCT AGG^CCT
TfiI 2201 GATTC G^A[AT]TC
Van91I 1465 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1575 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AocI 266 CCTGAGG CC^T[ACGT]AGG
1367 CCTCAGG
BanI 398 GGTGCC G^G[CT][GA]CC
1004 GGCACC
BsaI 1553 GGTCTC GGTCTC(1/5)
1848 GGTCTC
BsaWI 140 ACCGGA [AT]^CCGG[AT]
206 TCCGGA
BsiEI 1048 CGATCG CG[GA][CT]^CG
1843 CGGTCG
BspMI 1192 ACCTGC ACCTGC(4/8)
2099 ACCTGC
Bsu36I 266 CCTGAGG CC^T[ACGT]AGG
1367 CCTCAGG
DsaI 63 CCATGG C^C[GA][CT]GG
2292 CCGTGG
Eco81I 266 CCTGAGG CC^T[ACGT]AGG
1367 CCTCAGG
MaeII 652 ACGT A^CGT
1623 ACGT
XcmI 1306 CCAGCCTGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1818 CCATTGCTCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BamHI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII