##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HgaI Hin1I HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MseI MunI NarI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Alw44I 673 GTGCAC G^TGCAC
ApaLI 673 GTGCAC G^TGCAC
AvaI 1346 CTCGAG C^[CT]CG[GA]G
AviII 1246 TGCGCA TGC^GCA
BalI 110 TGGCCA TGG^CCA
BanII 699 GAGCTC G[GA]GC[CT]^C
BciVI 524 GTATCC GTATCC(6/5)
BglI 1143 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 833 AGATCT A^GATCT
BsmI 1056 GAATGC GAATGC(1/-1)
BsoBI 1346 CTCGAG C^[CT]CG[GA]G
BspHI 1097 TCATGA T^CATGA
BsrBI 450 CCGCTC CCGCTC(-3/-3)
BsrDI 801 CATTGC GCAATG(2/0)
BsrFI 55 GCCGGC [GA]^CCGG[CT]
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
DraIII 246 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 606 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1246 TGCGCA TGC^GCA
HaeII 257 AGCGCC [GA]GCGC^[CT]
HincII 124 GTCAAC GT[CT]^[GA]AC
HindII 124 GTCAAC GT[CT]^[GA]AC
MaeII 1735 ACGT A^CGT
MluNI 110 TGGCCA TGG^CCA
MscI 110 TGGCCA TGG^CCA
NaeI 55 GCCGGC GCC^GGC
NdeI 1537 CATATG CA^TATG
NgoMIV 55 GCCGGC G^CCGGC
PaeR7I 1346 CTCGAG C^TCGAG
PvuII 532 CAGCTG CAG^CTG
RcaI 1097 TCATGA T^CATGA
SacI 699 GAGCTC GAGCT^C
SapI 498 GAAGAGC GCTCTTC(1/4)
Tsp509I 1693 AATT ^AATT
XhoI 1346 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 471 GGTACC G^GTACC
1030 GGTACC
AccII 34 CGCG CG^CG
1192 CGCG
Asp718I 471 GGTACC G^GTACC
1030 GGTACC
AspHI 673 GTGCAC G[AT]GC[AT]^C
699 GAGCTC
BbsI 901 GAAGAC GAAGAC(2/6)
1492 GTCTTC
BcnI 80 CCGGG CC^[GC]GG
493 CCGGG
BfaI 274 CTAG C^TAG
628 CTAG
BpuAI 901 GAAGAC GAAGAC(2/6)
1492 GTCTTC
BsaWI 371 ACCGGA [AT]^CCGG[AT]
1052 ACCGGA
BseRI 1135 GAGGAG GAGGAG(10/8)
1138 GAGGAG
BsiHKAI 673 GTGCAC G[AT]GC[AT]^C
699 GAGCTC
BspMI 905 ACCTGC ACCTGC(4/8)
211 GCAGGT
BstUI 34 CGCG CG^CG
1192 CGCG
EaeI 110 TGGCCA [CT]^GGCC[GA]
1188 TGGCCG
EarI 478 GAAGAG CTCTTC(1/4)
498 GAAGAG
KpnI 471 GGTACC GGTAC^C
1030 GGTACC
Ksp632I 478 GAAGAG CTCTTC(1/4)
498 GAAGAG
MaeI 274 CTAG C^TAG
628 CTAG
MspA1I 237 CAGCGG C[AC]G^C[GT]G
532 CAGCTG
MvnI 34 CGCG CG^CG
1192 CGCG
NciI 80 CCGGG CC^[GC]GG
493 CCGGG
NcoI 998 CCATGG C^CATGG
1406 CCATGG
PleI 1090 GACTC GAGTC(4/5)
1280 GACTC
PshAI 151 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
643 GACCACAGTC
PstI 209 CTGCAG CTGCA^G
1340 CTGCAG
StuI 1355 AGGCCT AGG^CCT
1617 AGGCCT
TaqI 1347 TCGA T^CGA
1479 TCGA
TthHB8I 1347 TCGA T^CGA
1479 TCGA
XcmI 558 CCAGCCTCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
565 CCAGATGGTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BbvI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI TseI Tsp45I TspRI
Van91I XhoII