##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaLI AscI
Asp700I Asp718I BalI BbsI BcgI
BciVI BfrI BglII BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SfiI SfuI SgrAI
SmlI SnaBI SpeI SspBI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 266 GGCGCC G[GA]^CG[CT]C
AflIII 399 ACGTGT A^C[GA][CT]GT
AhdI 1090 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 88 GGGCCC GGGCC^C
AseI 1057 ATTAAT AT^TAAT
AsnI 1057 ATTAAT AT^TAAT
AspEI 1090 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 329 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2111 CCTAGG C^CTAGG
BamHI 1395 GGATCC G^GATCC
BbeI 266 GGCGCC GGCGC^C
BbvCI 802 GCTGAGG CCTCAGC(-5/-2)
BlnI 2111 CCTAGG C^CTAGG
BsaHI 266 GGCGCC G[GA]^CG[CT]C
BsmBI 2158 GAGACG CGTCTC(1/5)
BsmI 1734 GAATGC GAATGC(1/-1)
BssSI 1003 CACGAG CACGAG(-5/-1)
DrdI 1806 GACAGGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1090 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1500 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 266 GGCGCC G[GA]^CG[CT]C
KasI 266 GGCGCC G^GCGCC
NarI 266 GGCGCC GG^CGCC
PflFI 329 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1057 ATTAAT AT^TAAT
SacI 16 GAGCTC GAGCT^C
SbfI 1278 CCTGCAGG CCTGCA^GG
ScaI 362 AGTACT AGT^ACT
SexAI 482 ACCAGGT A^CC[AT]GGT
SfoI 266 GGCGCC GGC^GCC
Sse8387I 1278 CCTGCAGG CCTGCA^GG
StuI 620 AGGCCT AGG^CCT
TaqI 327 TCGA T^CGA
Tth111I 329 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 327 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AfaI 363 GTAC GT^AC
378 GTAC
AocI 1014 CCTGAGG CC^T[ACGT]AGG
1294 CCTCAGG
Aor51HI 320 AGCGCT AGC^GCT
434 AGCGCT
AviII 290 TGCGCA TGC^GCA
1892 TGCGCA
BbrPI 398 CACGTG CAC^GTG
2241 CACGTG
BclI 1841 TGATCA T^GATCA
2136 TGATCA
BfaI 2112 CTAG C^TAG
2315 CTAG
BsaAI 398 CACGTG [CT]AC^GT[GA]
2241 CACGTG
BsgI 646 CTGCAC GTGCAG(16/14)
52 GTGCAG
BsiEI 224 CGGCCG CG[GA][CT]^CG
297 CGGCCG
BsmAI 2158 GAGAC GTCTC(1/5)
335 GTCTC
BsrBI 456 GAGCGG CCGCTC(-3/-3)
1850 GAGCGG
BssHII 177 GCGCGC G^CGCGC
179 GCGCGC
BstAPI 348 GCAGCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1359 GCACACGCTGC
BstEII 754 GGTGACC G^GT[ACGT]ACC
2296 GGTGACC
BstPI 754 GGTGACC G^GT[ACGT]ACC
2296 GGTGACC
BstYI 1287 AGATCC [GA]^GATC[CT]
1395 GGATCC
Bsu36I 1014 CCTGAGG CC^T[ACGT]AGG
1294 CCTCAGG
EagI 224 CGGCCG C^GGCCG
297 CGGCCG
EclXI 224 CGGCCG C^GGCCG
297 CGGCCG
Eco47III 320 AGCGCT AGC^GCT
434 AGCGCT
Eco52I 224 CGGCCG C^GGCCG
297 CGGCCG
Eco81I 1014 CCTGAGG CC^T[ACGT]AGG
1294 CCTCAGG
EcoO65I 754 GGTGACC G^GT[ACGT]ACC
2296 GGTGACC
EcoRI 2212 GAATTC G^AATTC
2332 GAATTC
FbaI 1841 TGATCA T^GATCA
2136 TGATCA
FspI 290 TGCGCA TGC^GCA
1892 TGCGCA
MaeI 2112 CTAG C^TAG
2315 CTAG
MflI 1287 AGATCC [GA]^GATC[CT]
1395 GGATCC
PflMI 1377 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1923 CCATGGCGTGG
PleI 1161 GACTC GAGTC(4/5)
2125 GAGTC
PmaCI 398 CACGTG CAC^GTG
2241 CACGTG
PmlI 398 CACGTG CAC^GTG
2241 CACGTG
PvuII 462 CAGCTG CAG^CTG
1799 CAGCTG
RsaI 363 GTAC GT^AC
378 GTAC
SphI 659 GCATGC GCATG^C
980 GCATGC
Van91I 1377 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1923 CCATGGCGTGG
XhoII 1287 AGATCC [GA]^GATC[CT]
1395 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PpuMI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XmaCI
XmaI