##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AviII
AvrII BamHI BbrPI BciVI BfrI
BlnI BseAI BsiEI BsiHKAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HpaI KpnI KspI MfeI MluI
MroI MunI NcoI NdeI NotI
NruI NsiI NspI NspV PacI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 304 GGCGCC G[GA]^CG[CT]C
AlwNI 1293 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 444 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 688 TGGCCA TGG^CCA
BanI 304 GGCGCC G^G[CT][GA]CC
BbeI 304 GGCGCC GGCGC^C
BcgI 1252 GCATGACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 697 TGATCA T^GATCA
BglII 1273 AGATCT A^GATCT
BlpI 657 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 657 GCTGAGC GC^T[ACGT]AGC
BsaAI 1346 CACGTA [CT]AC^GT[GA]
BsaBI 1289 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 304 GGCGCC G[GA]^CG[CT]C
BsaI 393 GAGACC GGTCTC(1/5)
BsiWI 1348 CGTACG C^GTACG
BsmI 801 GCATTC GAATGC(1/-1)
BspMI 1106 GCAGGT ACCTGC(4/8)
BssHII 32 GCGCGC G^CGCGC
CelII 657 GCTGAGC GC^T[ACGT]AGC
DraIII 1424 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 890 GAAGAG CTCTTC(1/4)
FbaI 697 TGATCA T^GATCA
HaeII 304 GGCGCC [GA]GCGC^[CT]
HgaI 315 GACGC GACGC(5/10)
Hin1I 304 GGCGCC G[GA]^CG[CT]C
HindIII 1590 AAGCTT A^AGCTT
KasI 304 GGCGCC G^GCGCC
Ksp632I 890 GAAGAG CTCTTC(1/4)
MamI 1289 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 688 TGGCCA TGG^CCA
MscI 688 TGGCCA TGG^CCA
NarI 304 GGCGCC GG^CGCC
PaeR7I 1870 CTCGAG C^TCGAG
PflFI 444 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1521 CTGCAG CTGCA^G
SapI 890 GAAGAGC GCTCTTC(1/4)
SexAI 430 ACCTGGT A^CC[AT]GGT
SfcI 1521 CTGCAG C^T[GA][CT]AG
SfiI 269 GGCCGCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 304 GGCGCC GGC^GCC
Tth111I 444 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1719 TCTAGA T^CTAGA
XhoI 1870 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 33 CGCG CG^CG
828 CGCG
ApaI 51 GGGCCC GGGCC^C
614 GGGCCC
BbsI 126 GTCTTC GAAGAC(2/6)
1368 GTCTTC
BbvCI 1600 CCTCAGC CCTCAGC(-5/-2)
1752 GCTGAGG
BpmI 1413 CTCCAG CTGGAG(16/14)
208 CTGGAG
BpuAI 126 GTCTTC GAAGAC(2/6)
1368 GTCTTC
BsaWI 370 ACCGGA [AT]^CCGG[AT]
463 TCCGGT
BseRI 1269 GAGGAG GAGGAG(10/8)
1317 GAGGAG
BsgI 1005 GTGCAG GTGCAG(16/14)
1242 GTGCAG
BsrDI 770 CATTGC GCAATG(2/0)
1193 CATTGC
BstUI 33 CGCG CG^CG
828 CGCG
DraI 1780 TTTAAA TTT^AAA
1818 TTTAAA
DraII 51 GGGCCCC [GA]G^G[ACGT]CC[CT]
1408 AGGCCCT
EcoNI 695 CCTGATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1400 CCTGTCCCAGG
EcoO109I 51 GGGCCCC [GA]G^G[ACGT]CC[CT]
1408 AGGCCCT
HincII 729 GTCAAC GT[CT]^[GA]AC
1434 GTCAAC
HindII 729 GTCAAC GT[CT]^[GA]AC
1434 GTCAAC
MaeII 637 ACGT A^CGT
1347 ACGT
MvnI 33 CGCG CG^CG
828 CGCG
NheI 13 GCTAGC G^CTAGC
1799 GCTAGC
PvuII 288 CAGCTG CAG^CTG
1363 CAGCTG
SmaI 274 CCCGGG CCC^GGG
1048 CCCGGG
SmlI 861 CTCAAG C^T[CT][GA]AG
1870 CTCGAG
TaqI 361 TCGA T^CGA
1871 TCGA
TthHB8I 361 TCGA T^CGA
1871 TCGA
XmaCI 274 CCCGGG C^CCGGG
1048 CCCGGG
XmaI 274 CCCGGG C^CCGGG
1048 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaI AvaII BanII
BbvI BcnI BfaI BglI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII XmnI