##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BlnI BsaBI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BstBI BstEII
BstPI Bsu36I ClaI DraI DraIII
DrdI EarI Eco47III Eco81I EcoNI
EcoO65I EcoRV FbaI FspI HincII
HindII HpaI KpnI Ksp632I MamI
MfeI MluI MluNI MroI MscI
MslI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Van91I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1389 GTATAC GT^[AC][GT]AC
AcsI 902 GAATTC [GA]^AATT[CT]
AhdI 1372 GACGAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 902 GAATTC [GA]^AATT[CT]
AspEI 1372 GACGAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 268 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 602 GTCTTC GAAGAC(2/6)
BlpI 664 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 664 GCTGAGC GC^T[ACGT]AGC
BpuAI 602 GTCTTC GAAGAC(2/6)
BsaAI 1454 CACGTA [CT]AC^GT[GA]
BsaWI 1741 TCCGGT [AT]^CCGG[AT]
BsgI 773 GTGCAG GTGCAG(16/14)
BsiEI 1321 CGGCCG CG[GA][CT]^CG
BsrBI 985 GAGCGG CCGCTC(-3/-3)
BsrDI 658 CATTGC GCAATG(2/0)
BssHII 192 GCGCGC G^CGCGC
BssSI 473 CACGAG CACGAG(-5/-1)
Bst1107I 1389 GTATAC GTA^TAC
BstZ17I 1389 GTATAC GTA^TAC
CelII 664 GCTGAGC GC^T[ACGT]AGC
CpoI 266 CGGACCG CG^G[AT]CCG
EagI 1321 CGGCCG C^GGCCG
Eam1105I 1372 GACGAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1321 CGGCCG C^GGCCG
Eco52I 1321 CGGCCG C^GGCCG
EcoRI 902 GAATTC G^AATTC
EcoT22I 1644 ATGCAT ATGCA^T
FseI 1322 GGCCGGCC GGCCGG^CC
HindIII 1034 AAGCTT A^AGCTT
KspI 24 CCGCGG CCGC^GG
NcoI 399 CCATGG C^CATGG
NsiI 1644 ATGCAT ATGCA^T
PflFI 268 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 141 CAGCTG CAG^CTG
RsrII 266 CGGACCG CG^G[AT]CCG
SacI 1267 GAGCTC GAGCT^C
SacII 24 CCGCGG CCGC^GG
SgrAI 234 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1098 GCATGC GCATG^C
Tth111I 268 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 780 CCATCTACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 490 GTAC GT^AC
721 GTAC
AlwI 1342 GATCC GGATC(4/5)
1669 GATCC
AlwNI 138 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
440 CAGGACCTG
ApaI 217 GGGCCC GGGCC^C
1082 GGGCCC
BbeI 61 GGCGCC GGCGC^C
238 GGCGCC
BfaI 170 CTAG C^TAG
300 CTAG
BglI 23 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
513 GCCCCACCGGC
BglII 549 AGATCT A^GATCT
852 AGATCT
BsaI 1289 GAGACC GGTCTC(1/5)
369 GGTCTC
BsmAI 1289 GAGAC GTCTC(1/5)
370 GTCTC
BspMI 444 ACCTGC ACCTGC(4/8)
1109 ACCTGC
BsrI 308 CCAGT ACTGG(1/-1)
969 CCAGT
BstXI 4 CCACGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1203 CCAGGGATGTGG
BstYI 549 AGATCT [GA]^GATC[CT]
852 AGATCT
EaeI 520 CGGCCA [CT]^GGCC[GA]
1321 CGGCCG
EcoT14I 399 CCATGG C^C[AT][AT]GG
894 CCTTGG
HaeII 61 GGCGCC [GA]GCGC^[CT]
238 GGCGCC
HinfI 824 GAGTC G^A[ACGT]TC
1352 GACTC
KasI 61 GGCGCC G^GCGCC
238 GGCGCC
MaeI 170 CTAG C^TAG
300 CTAG
MaeII 600 ACGT A^CGT
1455 ACGT
MflI 549 AGATCT [GA]^GATC[CT]
852 AGATCT
NarI 61 GGCGCC GG^CGCC
238 GGCGCC
PleI 1352 GACTC GAGTC(4/5)
824 GAGTC
RsaI 490 GTAC GT^AC
721 GTAC
SfoI 61 GGCGCC GGC^GCC
238 GGCGCC
SmlI 503 CTCAAG C^T[CT][GA]AG
701 CTCAAG
StyI 399 CCATGG C^C[AT][AT]GG
894 CCTTGG
TaqI 615 TCGA T^CGA
639 TCGA
Tsp509I 903 AATT ^AATT
1493 AATT
TspRI 950 CAGTG CA[GC]TG[ACGT][ACGT]^
964 CAGTG
TthHB8I 615 TCGA T^CGA
639 TCGA
XhoII 549 AGATCT [GA]^GATC[CT]
852 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
Tru9I TseI Tsp45I XmaCI XmaI