##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AcsI AflII
AgeI AhdI AlwI ApaI ApoI
AscI Asp700I Asp718I AspEI AspI
AviII BamHI BcgI BclI BfrI
BglII BlpI Bpu1102I BsaBI BsaI
BseAI BsiWI BsmAI BsmBI BspDI
BspEI BspHI BssHII BssSI BstAPI
BstBI BstEII BstPI BstYI CelII
ClaI CpoI DraIII DrdI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
KpnI MamI MfeI MflI MluI
MroI MunI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmeI PshAI
PvuI RcaI RsrII SacI SalI
SbfI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AclI 995 AACGTT AA^CGTT
AflIII 197 ACATGT A^C[GA][CT]GT
Alw44I 201 GTGCAC G^TGCAC
Aor51HI 122 AGCGCT AGC^GCT
ApaLI 201 GTGCAC G^TGCAC
AseI 1917 ATTAAT AT^TAAT
AsnI 1917 ATTAAT AT^TAAT
AvaI 811 CCCGAG C^[CT]CG[GA]G
AvrII 1778 CCTAGG C^CTAGG
BalI 629 TGGCCA TGG^CCA
BbrPI 583 CACGTG CAC^GTG
BbsI 955 GTCTTC GAAGAC(2/6)
BbvCI 670 CCTCAGC CCTCAGC(-5/-2)
BciVI 1457 GGATAC GTATCC(6/5)
BglI 1048 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1778 CCTAGG C^CTAGG
BpuAI 955 GTCTTC GAAGAC(2/6)
BsaAI 583 CACGTG [CT]AC^GT[GA]
BsaWI 332 TCCGGT [AT]^CCGG[AT]
BsiEI 267 CGGCCG CG[GA][CT]^CG
BsoBI 811 CCCGAG C^[CT]CG[GA]G
BspLU11I 197 ACATGT A^CATGT
BsrBI 110 CCGCTC CCGCTC(-3/-3)
BsrFI 265 GCCGGC [GA]^CCGG[CT]
Bst1107I 1543 GTATAC GTA^TAC
BstXI 302 CCAGCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1543 GTATAC GTA^TAC
Cfr10I 265 GCCGGC [GA]^CCGG[CT]
DraI 1882 TTTAAA TTT^AAA
EagI 267 CGGCCG C^GGCCG
EclXI 267 CGGCCG C^GGCCG
Eco47III 122 AGCGCT AGC^GCT
Eco52I 267 CGGCCG C^GGCCG
EcoNI 984 CCTAGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1778 CCTAGG C^C[AT][AT]GG
HincII 664 GTTGAC GT[CT]^[GA]AC
HindII 664 GTTGAC GT[CT]^[GA]AC
MluNI 629 TGGCCA TGG^CCA
MscI 629 TGGCCA TGG^CCA
MslI 1754 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 265 GCCGGC GCC^GGC
NgoMIV 265 GCCGGC G^CCGGC
PflMI 1548 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 583 CACGTG CAC^GTG
PmlI 583 CACGTG CAC^GTG
PshBI 1917 ATTAAT AT^TAAT
Psp1406I 995 AACGTT AA^CGTT
PvuII 487 CAGCTG CAG^CTG
SfiI 1047 GGCCAAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 941 CTCAAG C^T[CT][GA]AG
StuI 1781 AGGCCT AGG^CCT
StyI 1778 CCTAGG C^C[AT][AT]GG
Van91I 1548 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1891 CCACTCATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 748 GTCTAC GT^[AC][GT]AC
1543 GTATAC
AcyI 259 GGCGCC G[GA]^CG[CT]C
298 GGCGCC
AlwNI 512 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
862 CAGGACCTG
AocI 973 CCTGAGG CC^T[ACGT]AGG
1799 CCTAAGG
AspHI 201 GTGCAC G[AT]GC[AT]^C
936 GAGCAC
BbeI 259 GGCGCC GGCGC^C
298 GGCGCC
BcnI 226 CCGGG CC^[GC]GG
608 CCCGG
BsaHI 259 GGCGCC G[GA]^CG[CT]C
298 GGCGCC
BsiHKAI 201 GTGCAC G[AT]GC[AT]^C
936 GAGCAC
Bsp1407I 1480 TGTACA T^GTACA
1931 TGTACA
BsrDI 1009 GCAATG GCAATG(2/0)
1758 GCAATG
BsrGI 1480 TGTACA T^GTACA
1931 TGTACA
BstF5I 1862 CATCC GGATG(2/0)
1602 GGATG
Bsu36I 973 CCTGAGG CC^T[ACGT]AGG
1799 CCTAAGG
DpnII 285 GATC ^GATC
525 GATC
EaeI 267 CGGCCG [CT]^GGCC[GA]
629 TGGCCA
Eco81I 973 CCTGAGG CC^T[ACGT]AGG
1799 CCTAAGG
FokI 1862 CATCC GGATG(9/13)
1602 GGATG
Hin1I 259 GGCGCC G[GA]^CG[CT]C
298 GGCGCC
HinfI 350 GATTC G^A[ACGT]TC
1014 GATTC
KasI 259 GGCGCC G^GCGCC
298 GGCGCC
KspI 80 CCGCGG CCGC^GG
236 CCGCGG
MboI 285 GATC ^GATC
525 GATC
NarI 259 GGCGCC GG^CGCC
298 GGCGCC
NciI 226 CCGGG CC^[GC]GG
608 CCCGG
NdeII 285 GATC ^GATC
525 GATC
NheI 125 GCTAGC G^CTAGC
687 GCTAGC
PpuMI 98 GGGTCCT [GA]G^G[AT]CC[CT]
863 AGGACCT
PstI 477 CTGCAG CTGCA^G
682 CTGCAG
SacII 80 CCGCGG CCGC^GG
236 CCGCGG
SapI 424 GCTCTTC GCTCTTC(1/4)
1650 GCTCTTC
Sau3AI 285 GATC ^GATC
525 GATC
SfoI 259 GGCGCC GGC^GCC
298 GGCGCC
SphI 695 GCATGC GCATG^C
1608 GCATGC
SspBI 1480 TGTACA T^GTACA
1931 TGTACA
SspI 1002 AATATT AAT^ATT
1535 AATATT
TfiI 350 GATTC G^A[AT]TC
1014 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanI BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EarI EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NlaIII NlaIV NspI RsaI Sau96I
ScrFI SfaNI SfcI Tru9I TseI
Tsp45I Tsp509I TspRI