##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI BamHI BbeI BbrPI BbvCI
BciVI BclI BglI BglII BsaAI
BsaBI BsaHI BsaI BsgI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstYI BstZ17I ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FbaI FseI Hin1I
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NsiI NspI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Van91I
XcmI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AfaI 644 GTAC GT^AC
Asp700I 1388 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1623 TGCGCA TGC^GCA
AvrII 1436 CCTAGG C^CTAGG
BalI 378 TGGCCA TGG^CCA
BanI 924 GGTGCC G^G[CT][GA]CC
BcgI 1351 GCAGGCCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1436 CCTAGG C^CTAGG
BlpI 1699 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1699 GCTGAGC GC^T[ACGT]AGC
BsiEI 207 CGGCCG CG[GA][CT]^CG
BsmI 1399 GCATTC GAATGC(1/-1)
BspMI 1061 GCAGGT ACCTGC(4/8)
BsrFI 849 ACCGGC [GA]^CCGG[CT]
BstBI 59 TTCGAA TT^CGAA
BstEII 679 GGTTACC G^GT[ACGT]ACC
BstPI 679 GGTTACC G^GT[ACGT]ACC
CelII 1699 GCTGAGC GC^T[ACGT]AGC
Cfr10I 849 ACCGGC [GA]^CCGG[CT]
EagI 207 CGGCCG C^GGCCG
EclXI 207 CGGCCG C^GGCCG
Eco52I 207 CGGCCG C^GGCCG
EcoNI 1581 CCTCTTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 679 GGTTACC G^GT[ACGT]ACC
FspI 1623 TGCGCA TGC^GCA
HaeII 29 AGCGCC [GA]GCGC^[CT]
HgaI 1015 GCGTC GACGC(5/10)
HincII 827 GTCAAC GT[CT]^[GA]AC
HindII 827 GTCAAC GT[CT]^[GA]AC
HindIII 1409 AAGCTT A^AGCTT
MaeII 642 ACGT A^CGT
MluNI 378 TGGCCA TGG^CCA
MscI 378 TGGCCA TGG^CCA
MslI 886 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 33 CCATGG C^CATGG
NruI 116 TCGCGA TCG^CGA
NspV 59 TTCGAA TT^CGAA
RsaI 644 GTAC GT^AC
SacI 569 GAGCTC GAGCT^C
SexAI 1518 ACCTGGT A^CC[AT]GGT
SfuI 59 TTCGAA TT^CGAA
SmaI 1071 CCCGGG CCC^GGG
XbaI 835 TCTAGA T^CTAGA
XmaCI 1071 CCCGGG C^CCGGG
XmaI 1071 CCCGGG C^CCGGG
XmnI 1388 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 1054 TCCGGA T^CCGGA
1287 TCCGGA
AcsI 764 AAATTC [GA]^AATT[CT]
1649 AAATTC
AflII 686 CTTAAG C^TTAAG
1412 CTTAAG
AocI 1130 CCTGAGG CC^T[ACGT]AGG
1674 CCTGAGG
ApoI 764 AAATTC [GA]^AATT[CT]
1649 AAATTC
AspI 401 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
962 GACTGTGTC
BfrI 686 CTTAAG C^TTAAG
1412 CTTAAG
BseAI 1054 TCCGGA T^CCGGA
1287 TCCGGA
BspEI 1054 TCCGGA T^CCGGA
1287 TCCGGA
Bsu36I 1130 CCTGAGG CC^T[ACGT]AGG
1674 CCTGAGG
DraIII 1088 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1705 CACATTGTG
EaeI 207 CGGCCG [CT]^GGCC[GA]
378 TGGCCA
EarI 566 GAAGAG CTCTTC(1/4)
1388 GAAGAG
Eco81I 1130 CCTGAGG CC^T[ACGT]AGG
1674 CCTGAGG
FauI 186 CCCGC CCCGC(4/6)
1478 CCCGC
HphI 1486 GGTGA GGTGA(8/7)
99 TCACC
Ksp632I 566 GAAGAG CTCTTC(1/4)
1388 GAAGAG
MroI 1054 TCCGGA T^CCGGA
1287 TCCGGA
PflFI 401 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
962 GACTGTGTC
PstI 230 CTGCAG CTGCA^G
1321 CTGCAG
SapI 566 GAAGAGC GCTCTTC(1/4)
1388 GAAGAGC
SfcI 230 CTGCAG C^T[GA][CT]AG
1321 CTGCAG
TfiI 337 GATTC G^A[AT]TC
802 GATTC
Tsp509I 765 AATT ^AATT
1650 AATT
Tth111I 401 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
962 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII Sau3AI Sau96I ScrFI SfaNI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I