##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       BamHI       BbeI        BbrPI       BbvCI       
BciVI       BclI        BglI        BglII       BsaAI       
BsaBI       BsaHI       BsaI        BsgI        BsiWI       
BsmBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstYI       BstZ17I     ClaI        CpoI        
DraI        DrdI        Eam1105I    Eco47III    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        Hin1I       
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspI        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Van91I      
XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AfaI          644  GTAC                 GT^AC
Asp700I      1388  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1623  TGCGCA               TGC^GCA
AvrII        1436  CCTAGG               C^CTAGG
BalI          378  TGGCCA               TGG^CCA
BanI          924  GGTGCC               G^G[CT][GA]CC
BcgI         1351  GCAGGCCATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         1436  CCTAGG               C^CTAGG
BlpI         1699  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1699  GCTGAGC              GC^T[ACGT]AGC
BsiEI         207  CGGCCG               CG[GA][CT]^CG
BsmI         1399  GCATTC               GAATGC(1/-1)
BspMI        1061  GCAGGT               ACCTGC(4/8)
BsrFI         849  ACCGGC               [GA]^CCGG[CT]
BstBI          59  TTCGAA               TT^CGAA
BstEII        679  GGTTACC              G^GT[ACGT]ACC
BstPI         679  GGTTACC              G^GT[ACGT]ACC
CelII        1699  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        849  ACCGGC               [GA]^CCGG[CT]
EagI          207  CGGCCG               C^GGCCG
EclXI         207  CGGCCG               C^GGCCG
Eco52I        207  CGGCCG               C^GGCCG
EcoNI        1581  CCTCTTGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       679  GGTTACC              G^GT[ACGT]ACC
FspI         1623  TGCGCA               TGC^GCA
HaeII          29  AGCGCC               [GA]GCGC^[CT]
HgaI         1015  GCGTC                GACGC(5/10)
HincII        827  GTCAAC               GT[CT]^[GA]AC
HindII        827  GTCAAC               GT[CT]^[GA]AC
HindIII      1409  AAGCTT               A^AGCTT
MaeII         642  ACGT                 A^CGT
MluNI         378  TGGCCA               TGG^CCA
MscI          378  TGGCCA               TGG^CCA
MslI          886  CACCTGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI           33  CCATGG               C^CATGG
NruI          116  TCGCGA               TCG^CGA
NspV           59  TTCGAA               TT^CGAA
RsaI          644  GTAC                 GT^AC
SacI          569  GAGCTC               GAGCT^C
SexAI        1518  ACCTGGT              A^CC[AT]GGT
SfuI           59  TTCGAA               TT^CGAA
SmaI         1071  CCCGGG               CCC^GGG
XbaI          835  TCTAGA               T^CTAGA
XmaCI        1071  CCCGGG               C^CCGGG
XmaI         1071  CCCGGG               C^CCGGG
XmnI         1388  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccIII       1054  TCCGGA               T^CCGGA
             1287  TCCGGA              
AcsI          764  AAATTC               [GA]^AATT[CT]
             1649  AAATTC              
AflII         686  CTTAAG               C^TTAAG
             1412  CTTAAG              
AocI         1130  CCTGAGG              CC^T[ACGT]AGG
             1674  CCTGAGG             
ApoI          764  AAATTC               [GA]^AATT[CT]
             1649  AAATTC              
AspI          401  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              962  GACTGTGTC           
BfrI          686  CTTAAG               C^TTAAG
             1412  CTTAAG              
BseAI        1054  TCCGGA               T^CCGGA
             1287  TCCGGA              
BspEI        1054  TCCGGA               T^CCGGA
             1287  TCCGGA              
Bsu36I       1130  CCTGAGG              CC^T[ACGT]AGG
             1674  CCTGAGG             
DraIII       1088  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1705  CACATTGTG           
EaeI          207  CGGCCG               [CT]^GGCC[GA]
              378  TGGCCA              
EarI          566  GAAGAG               CTCTTC(1/4)
             1388  GAAGAG              
Eco81I       1130  CCTGAGG              CC^T[ACGT]AGG
             1674  CCTGAGG             
FauI          186  CCCGC                CCCGC(4/6)
             1478  CCCGC               
HphI         1486  GGTGA                GGTGA(8/7)
               99  TCACC               
Ksp632I       566  GAAGAG               CTCTTC(1/4)
             1388  GAAGAG              
MroI         1054  TCCGGA               T^CCGGA
             1287  TCCGGA              
PflFI         401  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              962  GACTGTGTC           
PstI          230  CTGCAG               CTGCA^G
             1321  CTGCAG              
SapI          566  GAAGAGC              GCTCTTC(1/4)
             1388  GAAGAGC             
SfcI          230  CTGCAG               C^T[GA][CT]AG
             1321  CTGCAG              
TfiI          337  GATTC                G^A[AT]TC
              802  GATTC               
Tsp509I       765  AATT                 ^AATT
             1650  AATT                
Tth111I       401  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              962  GACTGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
ApaI        AspHI       AvaI        AvaII       BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BsaWI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        StuI        StyI        TaqI        Tru9I       
TseI        Tsp45I      TspRI       TthHB8I