##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspI AvrII BalI BamHI
BbrPI BcgI BciVI BclI BfrI
BlnI BsaAI BsaBI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrGI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI DraIII DrdI EcoRI EcoRV
FbaI FseI HindIII HpaI KpnI
MamI MfeI MluI MluNI MscI
MunI NdeI NheI NotI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1806 GACGTC GACGT^C
AccI 330 GTCTAC GT^[AC][GT]AC
AviII 36 TGCGCA TGC^GCA
BbvCI 831 CCTCAGC CCTCAGC(-5/-2)
BglII 2496 AGATCT A^GATCT
BlpI 1842 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1842 GCTAAGC GC^T[ACGT]AGC
BsaI 267 GAGACC GGTCTC(1/5)
BsgI 2000 GTGCAG GTGCAG(16/14)
BsmBI 1316 CGTCTC CGTCTC(1/5)
BsmI 2361 GAATGC GAATGC(1/-1)
BspMI 42 GCAGGT ACCTGC(4/8)
BsrBI 1831 CCGCTC CCGCTC(-3/-3)
BssHII 408 GCGCGC G^CGCGC
BssSI 1551 CACGAG CACGAG(-5/-1)
BstEII 2317 GGTCACC G^GT[ACGT]ACC
BstPI 2317 GGTCACC G^GT[ACGT]ACC
CelII 1842 GCTAAGC GC^T[ACGT]AGC
EcoO65I 2317 GGTCACC G^GT[ACGT]ACC
EcoT22I 2363 ATGCAT ATGCA^T
FspI 36 TGCGCA TGC^GCA
HincII 2145 GTCAAC GT[CT]^[GA]AC
HindII 2145 GTCAAC GT[CT]^[GA]AC
MaeII 1807 ACGT A^CGT
MseI 2018 TTAA T^TAA
NaeI 1733 GCCGGC GCC^GGC
NcoI 1466 CCATGG C^CATGG
NgoMIV 1733 GCCGGC G^CCGGC
NruI 661 TCGCGA TCG^CGA
NsiI 2363 ATGCAT ATGCA^T
PaeR7I 1308 CTCGAG C^TCGAG
PflMI 356 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1957 CTGCAG CTGCA^G
PvuII 2113 CAGCTG CAG^CTG
SfcI 1957 CTGCAG C^T[GA][CT]AG
SmaI 1585 CCCGGG CCC^GGG
StuI 1423 AGGCCT AGG^CCT
TaqI 1309 TCGA T^CGA
Tru9I 2018 TTAA T^TAA
TthHB8I 1309 TCGA T^CGA
Van91I 356 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1308 CTCGAG C^TCGAG
XmaCI 1585 CCCGGG C^CCGGG
XmaI 1585 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 728 TCCGGA T^CCGGA
1716 TCCGGA
AcsI 324 GAATTT [GA]^AATT[CT]
2104 AAATTT
AhdI 1182 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2268 GACCGTGGGTC
ApoI 324 GAATTT [GA]^AATT[CT]
2104 AAATTT
Asp700I 1505 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2397 GAAACTTTTC
AspEI 1182 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2268 GACCGTGGGTC
BbeI 1085 GGCGCC GGCGC^C
1581 GGCGCC
BseAI 728 TCCGGA T^CCGGA
1716 TCCGGA
BsiEI 1472 CGGCCG CG[GA][CT]^CG
1735 CGGCCG
BspEI 728 TCCGGA T^CCGGA
1716 TCCGGA
BsrDI 2335 GCAATG GCAATG(2/0)
2436 GCAATG
BsrFI 20 ACCGGC [GA]^CCGG[CT]
1733 GCCGGC
BstXI 674 CCAGGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1769 CCACGCCCCTGG
Cfr10I 20 ACCGGC [GA]^CCGG[CT]
1733 GCCGGC
EaeI 1472 CGGCCG [CT]^GGCC[GA]
1735 CGGCCG
EagI 1472 CGGCCG C^GGCCG
1735 CGGCCG
Eam1105I 1182 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2268 GACCGTGGGTC
EclXI 1472 CGGCCG C^GGCCG
1735 CGGCCG
Eco52I 1472 CGGCCG C^GGCCG
1735 CGGCCG
KasI 1085 GGCGCC G^GCGCC
1581 GGCGCC
KspI 1054 CCGCGG CCGC^GG
1388 CCGCGG
MroI 728 TCCGGA T^CCGGA
1716 TCCGGA
NarI 1085 GGCGCC GG^CGCC
1581 GGCGCC
SacI 200 GAGCTC GAGCT^C
657 GAGCTC
SacII 1054 CCGCGG CCGC^GG
1388 CCGCGG
SfoI 1085 GGCGCC GGC^GCC
1581 GGCGCC
SmlI 1308 CTCGAG C^T[CT][GA]AG
2095 CTCAAG
XmnI 1505 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2397 GAAACTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI Eco47III
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI TseI Tsp45I Tsp509I TspRI
XcmI XhoII