##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AgeI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BcgI BciVI BclI BglII
BlnI BsaBI BseAI BspDI BspEI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII KpnI MamI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StuI SwaI TfiI
Tsp509I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 1089 GACGTC GACGT^C
AflII 851 CTTAAG C^TTAAG
AhdI 1032 GACCGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1526 GTGCAC G^TGCAC
AocI 1753 CCTGAGG CC^T[ACGT]AGG
ApaLI 1526 GTGCAC G^TGCAC
AspEI 1032 GACCGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1526 GTGCAC G[AT]GC[AT]^C
BalI 292 TGGCCA TGG^CCA
BbeI 219 GGCGCC GGCGC^C
BbrPI 1644 CACGTG CAC^GTG
BbvCI 24 GCTGAGG CCTCAGC(-5/-2)
BfaI 1350 CTAG C^TAG
BfrI 851 CTTAAG C^TTAAG
BlpI 107 GCTGAGC GC^T[ACGT]AGC
BpmI 1317 CTGGAG CTGGAG(16/14)
Bpu1102I 107 GCTGAGC GC^T[ACGT]AGC
BsaI 459 GAGACC GGTCTC(1/5)
BsaWI 1922 TCCGGT [AT]^CCGG[AT]
BsgI 1125 GTGCAG GTGCAG(16/14)
BsiHKAI 1526 GTGCAC G[AT]GC[AT]^C
BsiWI 596 CGTACG C^GTACG
BsmBI 159 GAGACG CGTCTC(1/5)
BsmI 748 GCATTC GAATGC(1/-1)
Bsp1407I 625 TGTACA T^GTACA
BspHI 1913 TCATGA T^CATGA
BspLU11I 362 ACATGT A^CATGT
BspMI 1737 GCAGGT ACCTGC(4/8)
BsrDI 952 CATTGC GCAATG(2/0)
BsrGI 625 TGTACA T^GTACA
BstAPI 1528 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1753 CCTGAGG CC^T[ACGT]AGG
CelII 107 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1032 GACCGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1753 CCTGAGG CC^T[ACGT]AGG
EcoT14I 709 CCATGG C^C[AT][AT]GG
HaeII 219 GGCGCC [GA]GCGC^[CT]
HpaI 1497 GTTAAC GTT^AAC
KasI 219 GGCGCC G^GCGCC
MaeI 1350 CTAG C^TAG
MluNI 292 TGGCCA TGG^CCA
MscI 292 TGGCCA TGG^CCA
NarI 219 GGCGCC GG^CGCC
NcoI 709 CCATGG C^CATGG
NdeI 1598 CATATG CA^TATG
PmaCI 1644 CACGTG CAC^GTG
PmlI 1644 CACGTG CAC^GTG
PstI 310 CTGCAG CTGCA^G
RcaI 1913 TCATGA T^CATGA
SapI 718 GAAGAGC GCTCTTC(1/4)
SfcI 310 CTGCAG C^T[GA][CT]AG
SfoI 219 GGCGCC GGC^GCC
SmlI 851 CTTAAG C^T[CT][GA]AG
SphI 1418 GCATGC GCATG^C
SspBI 625 TGTACA T^GTACA
StyI 709 CCATGG C^C[AT][AT]GG
TaqI 1742 TCGA T^CGA
TthHB8I 1742 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 362 ACATGT A^C[GA][CT]GT
1964 ACGTGT
ApaI 1709 GGGCCC GGGCC^C
1871 GGGCCC
AspI 315 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1635 GACGAAGTC
BsaAI 1644 CACGTG [CT]AC^GT[GA]
1963 TACGTG
BsrFI 101 GCCGGT [GA]^CCGG[CT]
1530 ACCGGC
BstEII 275 GGTGACC G^GT[ACGT]ACC
1670 GGTGACC
BstPI 275 GGTGACC G^GT[ACGT]ACC
1670 GGTGACC
BstXI 328 CCACGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
437 CCACACAGATGG
BstYI 793 GGATCT [GA]^GATC[CT]
1459 AGATCC
Cfr10I 101 GCCGGT [GA]^CCGG[CT]
1530 ACCGGC
EcoNI 20 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
779 CCTCATCCAGG
EcoO65I 275 GGTGACC G^GT[ACGT]ACC
1670 GGTGACC
MflI 793 GGATCT [GA]^GATC[CT]
1459 AGATCC
NspI 362 ACATGT [GA]CATG^[CT]
1418 GCATGC
PflFI 315 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1635 GACGAAGTC
PpuMI 500 AGGTCCT [GA]G^G[AT]CC[CT]
565 GGGACCC
SmaI 201 CCCGGG CCC^GGG
1868 CCCGGG
Tth111I 315 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1635 GACGAAGTC
XcmI 280 CCACAGTGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
536 CCAAGAAAAAGGTGG
XhoII 793 GGATCT [GA]^GATC[CT]
1459 AGATCC
XmaCI 201 CCCGGG C^CCGGG
1868 CCCGGG
XmaI 201 CCCGGG C^CCGGG
1868 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanI
BanII BbsI BbvI BcnI BglI
BmyI BpuAI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NotI PleI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp45I TspRI