################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccIII AflII AflIII AgeI Alw44I AlwNI AocI ApaI ApaLI AscI Asp700I Asp718I AspHI AvaI AviII AvrII BbrPI BciVI BfrI BglI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraIII EagI EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AclI 1658 AACGTT AA^CGTT AfaI 1542 GTAC GT^AC AhdI 532 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 16 AGCGCT AGC^GCT AseI 604 ATTAAT AT^TAAT AsnI 604 ATTAAT AT^TAAT AspEI 532 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1850 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 943 GGATCC G^GATCC BbeI 290 GGCGCC GGCGC^C BbvCI 1945 CCTCAGC CCTCAGC(-5/-2) BcgI 1934 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 12 GCTCAGC GC^T[ACGT]AGC BpmI 1876 CTGGAG CTGGAG(16/14) Bpu1102I 12 GCTCAGC GC^T[ACGT]AGC BseRI 2076 CTCCTC GAGGAG(10/8) BstAPI 195 GCATCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2088 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 12 GCTCAGC GC^T[ACGT]AGC Eam1105I 532 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 16 AGCGCT AGC^GCT EcoRV 907 GATATC GAT^ATC KasI 290 GGCGCC G^GCGCC NarI 290 GGCGCC GG^CGCC PflFI 1850 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1296 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 604 ATTAAT AT^TAAT Psp1406I 1658 AACGTT AA^CGTT RsaI 1542 GTAC GT^AC SfaNI 195 GCATC GCATC(5/9) SfcI 1265 CTACAG C^T[GA][CT]AG SfoI 290 GGCGCC GGC^GCC StuI 1611 AGGCCT AGG^CCT Tth111I 1850 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1296 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 513 TCTAGA T^CTAGA XcmI 1552 CCATTTGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 290 GGCGCC G[GA]^CG[CT]C 307 GACGCC BalI 843 TGGCCA TGG^CCA 1205 TGGCCA BanI 290 GGCGCC G^G[CT][GA]CC 1338 GGCACC BclI 1047 TGATCA T^GATCA 1682 TGATCA BsaHI 290 GGCGCC G[GA]^CG[CT]C 307 GACGCC BsaI 216 GAGACC GGTCTC(1/5) 2046 GGTCTC BsmBI 1917 CGTCTC CGTCTC(1/5) 2016 GAGACG BsrFI 175 GCCGGT [GA]^CCGG[CT] 235 GCCGGT BstYI 943 GGATCC [GA]^GATC[CT] 1691 GGATCT Cfr10I 175 GCCGGT [GA]^CCGG[CT] 235 GCCGGT DraI 409 TTTAAA TTT^AAA 2406 TTTAAA DraII 297 GGGTCCT [GA]G^G[ACGT]CC[CT] 1576 GGGTCCC DrdI 256 GACAGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1776 GACTTGCTAGTC EaeI 843 TGGCCA [CT]^GGCC[GA] 1205 TGGCCA EcoO109I 297 GGGTCCT [GA]G^G[ACGT]CC[CT] 1576 GGGTCCC FbaI 1047 TGATCA T^GATCA 1682 TGATCA HaeII 16 AGCGCT [GA]GCGC^[CT] 290 GGCGCC HgaI 54 GACGC GACGC(5/10) 307 GACGC Hin1I 290 GGCGCC G[GA]^CG[CT]C 307 GACGCC MaeII 100 ACGT A^CGT 1659 ACGT MflI 943 GGATCC [GA]^GATC[CT] 1691 GGATCT MluNI 843 TGGCCA TGG^CCA 1205 TGGCCA MscI 843 TGGCCA TGG^CCA 1205 TGGCCA MslI 272 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1988 CACCTGGGTG NspI 646 GCATGT [GA]CATG^[CT] 1975 GCATGT PpuMI 297 GGGTCCT [GA]G^G[AT]CC[CT] 1576 GGGTCCC PvuII 1201 CAGCTG CAG^CTG 2121 CAGCTG SmlI 590 CTCAAG C^T[CT][GA]AG 1665 CTCAAG SspI 2209 AATATT AAT^ATT 2442 AATATT XhoII 943 GGATCC [GA]^GATC[CT] 1691 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApoI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpuAI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KspI MaeI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI SacII Sau3AI Sau96I ScrFI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I