##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI Alw44I AlwNI AocI
ApaI ApaLI AscI Asp700I Asp718I
AspHI AvaI AviII AvrII BbrPI
BciVI BfrI BglI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraIII EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I MamI MfeI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PstI PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StyI SwaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 1658 AACGTT AA^CGTT
AfaI 1542 GTAC GT^AC
AhdI 532 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 16 AGCGCT AGC^GCT
AseI 604 ATTAAT AT^TAAT
AsnI 604 ATTAAT AT^TAAT
AspEI 532 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1850 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 943 GGATCC G^GATCC
BbeI 290 GGCGCC GGCGC^C
BbvCI 1945 CCTCAGC CCTCAGC(-5/-2)
BcgI 1934 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 12 GCTCAGC GC^T[ACGT]AGC
BpmI 1876 CTGGAG CTGGAG(16/14)
Bpu1102I 12 GCTCAGC GC^T[ACGT]AGC
BseRI 2076 CTCCTC GAGGAG(10/8)
BstAPI 195 GCATCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2088 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 12 GCTCAGC GC^T[ACGT]AGC
Eam1105I 532 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 16 AGCGCT AGC^GCT
EcoRV 907 GATATC GAT^ATC
KasI 290 GGCGCC G^GCGCC
NarI 290 GGCGCC GG^CGCC
PflFI 1850 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1296 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 604 ATTAAT AT^TAAT
Psp1406I 1658 AACGTT AA^CGTT
RsaI 1542 GTAC GT^AC
SfaNI 195 GCATC GCATC(5/9)
SfcI 1265 CTACAG C^T[GA][CT]AG
SfoI 290 GGCGCC GGC^GCC
StuI 1611 AGGCCT AGG^CCT
Tth111I 1850 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1296 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 513 TCTAGA T^CTAGA
XcmI 1552 CCATTTGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 290 GGCGCC G[GA]^CG[CT]C
307 GACGCC
BalI 843 TGGCCA TGG^CCA
1205 TGGCCA
BanI 290 GGCGCC G^G[CT][GA]CC
1338 GGCACC
BclI 1047 TGATCA T^GATCA
1682 TGATCA
BsaHI 290 GGCGCC G[GA]^CG[CT]C
307 GACGCC
BsaI 216 GAGACC GGTCTC(1/5)
2046 GGTCTC
BsmBI 1917 CGTCTC CGTCTC(1/5)
2016 GAGACG
BsrFI 175 GCCGGT [GA]^CCGG[CT]
235 GCCGGT
BstYI 943 GGATCC [GA]^GATC[CT]
1691 GGATCT
Cfr10I 175 GCCGGT [GA]^CCGG[CT]
235 GCCGGT
DraI 409 TTTAAA TTT^AAA
2406 TTTAAA
DraII 297 GGGTCCT [GA]G^G[ACGT]CC[CT]
1576 GGGTCCC
DrdI 256 GACAGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1776 GACTTGCTAGTC
EaeI 843 TGGCCA [CT]^GGCC[GA]
1205 TGGCCA
EcoO109I 297 GGGTCCT [GA]G^G[ACGT]CC[CT]
1576 GGGTCCC
FbaI 1047 TGATCA T^GATCA
1682 TGATCA
HaeII 16 AGCGCT [GA]GCGC^[CT]
290 GGCGCC
HgaI 54 GACGC GACGC(5/10)
307 GACGC
Hin1I 290 GGCGCC G[GA]^CG[CT]C
307 GACGCC
MaeII 100 ACGT A^CGT
1659 ACGT
MflI 943 GGATCC [GA]^GATC[CT]
1691 GGATCT
MluNI 843 TGGCCA TGG^CCA
1205 TGGCCA
MscI 843 TGGCCA TGG^CCA
1205 TGGCCA
MslI 272 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1988 CACCTGGGTG
NspI 646 GCATGT [GA]CATG^[CT]
1975 GCATGT
PpuMI 297 GGGTCCT [GA]G^G[AT]CC[CT]
1576 GGGTCCC
PvuII 1201 CAGCTG CAG^CTG
2121 CAGCTG
SmlI 590 CTCAAG C^T[CT][GA]AG
1665 CTCAAG
SspI 2209 AATATT AAT^ATT
2442 AATATT
XhoII 943 GGATCC [GA]^GATC[CT]
1691 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpuAI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
KspI MaeI MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI SacII Sau3AI Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I