##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I AocI ApaLI AscI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BcnI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoRI
EcoRV FseI FspI HapII HpaI
HpaII KasI KspI MamI MluI
MluNI MroI MscI MspI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SacII
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 948 GGTACC G^GTACC
AccII 1095 CGCG CG^CG
AflII 1900 CTTAAG C^TTAAG
Aor51HI 874 AGCGCT AGC^GCT
ApaI 1749 GGGCCC GGGCC^C
Asp718I 948 GGTACC G^GTACC
BanII 1749 GGGCCC G[GA]GC[CT]^C
BclI 1226 TGATCA T^GATCA
BfrI 1900 CTTAAG C^TTAAG
BpmI 2117 CTCCAG CTGGAG(16/14)
BsaI 1144 GAGACC GGTCTC(1/5)
BseRI 1072 GAGGAG GAGGAG(10/8)
Bsp1407I 559 TGTACA T^GTACA
BspHI 602 TCATGA T^CATGA
BspLU11I 2188 ACATGT A^CATGT
BspMI 760 ACCTGC ACCTGC(4/8)
BsrGI 559 TGTACA T^GTACA
BstBI 131 TTCGAA TT^CGAA
BstUI 1095 CGCG CG^CG
BstYI 1552 GGATCT [GA]^GATC[CT]
DraI 1690 TTTAAA TTT^AAA
EaeI 1052 CGGCCA [CT]^GGCC[GA]
EarI 1269 GAAGAG CTCTTC(1/4)
Eco47III 874 AGCGCT AGC^GCT
FbaI 1226 TGATCA T^GATCA
HaeII 874 AGCGCT [GA]GCGC^[CT]
HindIII 1371 AAGCTT A^AGCTT
KpnI 948 GGTACC GGTAC^C
Ksp632I 1269 GAAGAG CTCTTC(1/4)
MflI 1552 GGATCT [GA]^GATC[CT]
MvnI 1095 CGCG CG^CG
NcoI 518 CCATGG C^CATGG
NspI 2188 ACATGT [GA]CATG^[CT]
NspV 131 TTCGAA TT^CGAA
PpuMI 391 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 260 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 262 CTGCAG CTGCA^G
RcaI 602 TCATGA T^CATGA
SalI 1803 GTCGAC G^TCGAC
SexAI 2079 ACCTGGT A^CC[AT]GGT
SfuI 131 TTCGAA TT^CGAA
SspBI 559 TGTACA T^GTACA
StuI 1394 AGGCCT AGG^CCT
SwaI 1689 ATTTAAAT ATTT^AAAT
XcmI 1960 CCATTGGTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1552 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 57 GTCTAC GT^[AC][GT]AC
1803 GTCGAC
AcyI 301 GACGCC G[GA]^CG[CT]C
331 GACGCC
AflIII 1481 ACGTGT A^C[GA][CT]GT
2188 ACATGT
AlwI 1933 GATCC GGATC(4/5)
1552 GGATC
AlwNI 703 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1412 CAGATACTG
AspHI 89 GTGCTC G[AT]GC[AT]^C
1545 GTGCTC
AvaI 654 CCCGAG C^[CT]CG[GA]G
743 CCCGAG
BanI 948 GGTACC G^G[CT][GA]CC
1193 GGCACC
BbsI 756 GAAGAC GAAGAC(2/6)
912 GAAGAC
BpuAI 756 GAAGAC GAAGAC(2/6)
912 GAAGAC
BsaHI 301 GACGCC G[GA]^CG[CT]C
331 GACGCC
BsiHKAI 89 GTGCTC G[AT]GC[AT]^C
1545 GTGCTC
BsoBI 654 CCCGAG C^[CT]CG[GA]G
743 CCCGAG
BstEII 435 GGTGACC G^GT[ACGT]ACC
522 GGTCACC
BstPI 435 GGTGACC G^GT[ACGT]ACC
522 GGTCACC
BstXI 491 CCATCACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1055 CCAACGGCATGG
DraII 391 AGGACCC [GA]G^G[ACGT]CC[CT]
1748 AGGGCCC
EcoO109I 391 AGGACCC [GA]G^G[ACGT]CC[CT]
1748 AGGGCCC
EcoO65I 435 GGTGACC G^GT[ACGT]ACC
522 GGTCACC
EcoT14I 518 CCATGG C^C[AT][AT]GG
551 CCAAGG
FauI 1093 CCCGC CCCGC(4/6)
1132 CCCGC
Hin1I 301 GACGCC G[GA]^CG[CT]C
331 GACGCC
HincII 1803 GTCGAC GT[CT]^[GA]AC
2022 GTTGAC
HindII 1803 GTCGAC GT[CT]^[GA]AC
2022 GTTGAC
MaeII 102 ACGT A^CGT
1481 ACGT
MspA1I 920 CAGCTG C[AC]G^C[GT]G
2224 CAGCTG
PvuII 920 CAGCTG CAG^CTG
2224 CAGCTG
SfcI 262 CTGCAG C^T[GA][CT]AG
1578 CTACAG
StyI 518 CCATGG C^C[AT][AT]GG
551 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AseI AsnI AvaII BbvI BfaI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EcoRII EcoT22I Fnu4HI
FokI HaeIII HgaI HhaI HinP1I
HinfI HphI ItaI MaeI MaeIII
MboI MboII MfeI MnlI MseI
MslI MunI MvaI MwoI NdeII
NlaIII NlaIV NsiI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I