##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AccI AccIII AclI AhdI AocI
Aor51HI AscI AseI AsnI AspEI
AvaII AvrII BamHI BbvCI BcgI
BciVI BcnI BglII BlnI BsaI
BseAI BsiEI BsiWI BsmBI BspEI
BspHI BspLU11I BspMI Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoT22I
FseI HpaI MfeI MluI MroI
MunI NaeI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PpuMI PshBI
Psp1406I PvuI RcaI RsrII SalI
SexAI SfuI SgrAI SmaI SnaBI
SpeI SphI SspI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 2196 GACGTC GACGT^C
Acc65I 2306 GGTACC G^GTACC
AflII 1982 CTTAAG C^TTAAG
AflIII 1072 ACGTGT A^C[GA][CT]GT
Alw44I 117 GTGCAC G^TGCAC
AlwI 1123 GATCC GGATC(4/5)
ApaLI 117 GTGCAC G^TGCAC
Asp718I 2306 GGTACC G^GTACC
AspI 44 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1949 TGCGCA TGC^GCA
BbeI 210 GGCGCC GGCGC^C
BbrPI 1071 CACGTG CAC^GTG
BfaI 2327 CTAG C^TAG
BfrI 1982 CTTAAG C^TTAAG
BlpI 1397 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1397 GCTGAGC GC^T[ACGT]AGC
BsaAI 1071 CACGTG [CT]AC^GT[GA]
BsaBI 344 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 523 TGTACA T^GTACA
BspDI 1314 ATCGAT AT^CGAT
BsrDI 1617 GCAATG GCAATG(2/0)
BsrGI 523 TGTACA T^GTACA
BssHII 27 GCGCGC G^CGCGC
BssSI 75 CACGAG CACGAG(-5/-1)
BstEII 1177 GGTTACC G^GT[ACGT]ACC
BstPI 1177 GGTTACC G^GT[ACGT]ACC
BstYI 1122 AGATCC [GA]^GATC[CT]
CelII 1397 GCTGAGC GC^T[ACGT]AGC
ClaI 1314 ATCGAT AT^CGAT
DraI 516 TTTAAA TTT^AAA
EcoO65I 1177 GGTTACC G^GT[ACGT]ACC
EcoRV 1317 GATATC GAT^ATC
FspI 1949 TGCGCA TGC^GCA
HaeII 210 GGCGCC [GA]GCGC^[CT]
HincII 2172 GTTGAC GT[CT]^[GA]AC
HindII 2172 GTTGAC GT[CT]^[GA]AC
KasI 210 GGCGCC G^GCGCC
KpnI 2306 GGTACC GGTAC^C
KspI 32 CCGCGG CCGC^GG
MaeI 2327 CTAG C^TAG
MamI 344 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1122 AGATCC [GA]^GATC[CT]
NarI 210 GGCGCC GG^CGCC
PflFI 44 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2225 CCATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1071 CACGTG CAC^GTG
PmeI 515 GTTTAAAC GTTT^AAAC
PmlI 1071 CACGTG CAC^GTG
PshAI 2037 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 32 CCGCGG CCGC^GG
SapI 2267 GCTCTTC GCTCTTC(1/4)
SfiI 56 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 210 GGCGCC GGC^GCC
SspBI 523 TGTACA T^GTACA
StuI 1978 AGGCCT AGG^CCT
Tth111I 44 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2225 CCATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2326 TCTAGA T^CTAGA
XhoII 1122 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AgeI 1903 ACCGGT A^CCGGT
2254 ACCGGT
ApaI 36 GGGCCC GGGCC^C
269 GGGCCC
Asp700I 699 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1545 GAACGATTTC
AvaI 39 CCCGAG C^[CT]CG[GA]G
52 CTCGGG
BanI 210 GGCGCC G^G[CT][GA]CC
2306 GGTACC
BclI 1592 TGATCA T^GATCA
1801 TGATCA
BglI 57 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
196 GCCGCACAGGC
BseRI 882 CTCCTC GAGGAG(10/8)
657 GAGGAG
BsoBI 39 CCCGAG C^[CT]CG[GA]G
52 CTCGGG
BsrBI 970 CCGCTC CCGCTC(-3/-3)
228 GAGCGG
BsrFI 1903 ACCGGT [GA]^CCGG[CT]
2254 ACCGGT
Cfr10I 1903 ACCGGT [GA]^CCGG[CT]
2254 ACCGGT
DraII 268 AGGGCCC [GA]G^G[ACGT]CC[CT]
748 AGGCCCT
EcoO109I 268 AGGGCCC [GA]G^G[ACGT]CC[CT]
748 AGGCCCT
FauI 34 GCGGG CCCGC(4/6)
1428 GCGGG
FbaI 1592 TGATCA T^GATCA
1801 TGATCA
HindIII 495 AAGCTT A^AGCTT
1746 AAGCTT
MaeII 1072 ACGT A^CGT
2197 ACGT
MslI 120 CACGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1790 CACAGAAATG
NcoI 61 CCATGG C^CATGG
1369 CCATGG
PinAI 1903 ACCGGT A^CCGGT
2254 ACCGGT
SacI 1023 GAGCTC GAGCT^C
1308 GAGCTC
SbfI 1031 CCTGCAGG CCTGCA^GG
1874 CCTGCAGG
ScaI 541 AGTACT AGT^ACT
1912 AGTACT
SmlI 959 CTTGAG C^T[CT][GA]AG
1982 CTTAAG
Sse8387I 1031 CCTGCAGG CCTGCA^GG
1874 CCTGCAGG
XcmI 2050 CCATGTTCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2065 CCAAGTGCCAGGTGG
XmnI 699 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1545 GAACGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwNI ApoI AspHI BalI
BanII BbsI BbvI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoNI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I