################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AccI AccIII AclI AhdI AocI Aor51HI AscI AseI AsnI AspEI AvaII AvrII BamHI BbvCI BcgI BciVI BcnI BglII BlnI BsaI BseAI BsiEI BsiWI BsmBI BspEI BspHI BspLU11I BspMI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoT22I FseI HpaI MfeI MluI MroI MunI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PpuMI PshBI Psp1406I PvuI RcaI RsrII SalI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 2196 GACGTC GACGT^C Acc65I 2306 GGTACC G^GTACC AflII 1982 CTTAAG C^TTAAG AflIII 1072 ACGTGT A^C[GA][CT]GT Alw44I 117 GTGCAC G^TGCAC AlwI 1123 GATCC GGATC(4/5) ApaLI 117 GTGCAC G^TGCAC Asp718I 2306 GGTACC G^GTACC AspI 44 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1949 TGCGCA TGC^GCA BbeI 210 GGCGCC GGCGC^C BbrPI 1071 CACGTG CAC^GTG BfaI 2327 CTAG C^TAG BfrI 1982 CTTAAG C^TTAAG BlpI 1397 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1397 GCTGAGC GC^T[ACGT]AGC BsaAI 1071 CACGTG [CT]AC^GT[GA] BsaBI 344 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 523 TGTACA T^GTACA BspDI 1314 ATCGAT AT^CGAT BsrDI 1617 GCAATG GCAATG(2/0) BsrGI 523 TGTACA T^GTACA BssHII 27 GCGCGC G^CGCGC BssSI 75 CACGAG CACGAG(-5/-1) BstEII 1177 GGTTACC G^GT[ACGT]ACC BstPI 1177 GGTTACC G^GT[ACGT]ACC BstYI 1122 AGATCC [GA]^GATC[CT] CelII 1397 GCTGAGC GC^T[ACGT]AGC ClaI 1314 ATCGAT AT^CGAT DraI 516 TTTAAA TTT^AAA EcoO65I 1177 GGTTACC G^GT[ACGT]ACC EcoRV 1317 GATATC GAT^ATC FspI 1949 TGCGCA TGC^GCA HaeII 210 GGCGCC [GA]GCGC^[CT] HincII 2172 GTTGAC GT[CT]^[GA]AC HindII 2172 GTTGAC GT[CT]^[GA]AC KasI 210 GGCGCC G^GCGCC KpnI 2306 GGTACC GGTAC^C KspI 32 CCGCGG CCGC^GG MaeI 2327 CTAG C^TAG MamI 344 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1122 AGATCC [GA]^GATC[CT] NarI 210 GGCGCC GG^CGCC PflFI 44 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2225 CCATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1071 CACGTG CAC^GTG PmeI 515 GTTTAAAC GTTT^AAAC PmlI 1071 CACGTG CAC^GTG PshAI 2037 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 32 CCGCGG CCGC^GG SapI 2267 GCTCTTC GCTCTTC(1/4) SfiI 56 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 210 GGCGCC GGC^GCC SspBI 523 TGTACA T^GTACA StuI 1978 AGGCCT AGG^CCT Tth111I 44 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2225 CCATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2326 TCTAGA T^CTAGA XhoII 1122 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AgeI 1903 ACCGGT A^CCGGT 2254 ACCGGT ApaI 36 GGGCCC GGGCC^C 269 GGGCCC Asp700I 699 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1545 GAACGATTTC AvaI 39 CCCGAG C^[CT]CG[GA]G 52 CTCGGG BanI 210 GGCGCC G^G[CT][GA]CC 2306 GGTACC BclI 1592 TGATCA T^GATCA 1801 TGATCA BglI 57 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 196 GCCGCACAGGC BseRI 882 CTCCTC GAGGAG(10/8) 657 GAGGAG BsoBI 39 CCCGAG C^[CT]CG[GA]G 52 CTCGGG BsrBI 970 CCGCTC CCGCTC(-3/-3) 228 GAGCGG BsrFI 1903 ACCGGT [GA]^CCGG[CT] 2254 ACCGGT Cfr10I 1903 ACCGGT [GA]^CCGG[CT] 2254 ACCGGT DraII 268 AGGGCCC [GA]G^G[ACGT]CC[CT] 748 AGGCCCT EcoO109I 268 AGGGCCC [GA]G^G[ACGT]CC[CT] 748 AGGCCCT FauI 34 GCGGG CCCGC(4/6) 1428 GCGGG FbaI 1592 TGATCA T^GATCA 1801 TGATCA HindIII 495 AAGCTT A^AGCTT 1746 AAGCTT MaeII 1072 ACGT A^CGT 2197 ACGT MslI 120 CACGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1790 CACAGAAATG NcoI 61 CCATGG C^CATGG 1369 CCATGG PinAI 1903 ACCGGT A^CCGGT 2254 ACCGGT SacI 1023 GAGCTC GAGCT^C 1308 GAGCTC SbfI 1031 CCTGCAGG CCTGCA^GG 1874 CCTGCAGG ScaI 541 AGTACT AGT^ACT 1912 AGTACT SmlI 959 CTTGAG C^T[CT][GA]AG 1982 CTTAAG Sse8387I 1031 CCTGCAGG CCTGCA^GG 1874 CCTGCAGG XcmI 2050 CCATGTTCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2065 CCAAGTGCCAGGTGG XmnI 699 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1545 GAACGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwNI ApoI AspHI BalI BanII BbsI BbvI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoNI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I