##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccII AclI AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AvrII BcgI BclI BlnI
BlpI Bpu1102I BsaBI BsiEI BsiWI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
CelII ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KspI MamI
MfeI MluI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 243 GTCTAC GT^[AC][GT]AC
AccIII 502 TCCGGA T^CCGGA
AcsI 1644 AAATTT [GA]^AATT[CT]
AflII 2004 CTTAAG C^TTAAG
AflIII 1096 ACGTGT A^C[GA][CT]GT
Alw44I 21 GTGCAC G^TGCAC
AocI 1884 CCTTAGG CC^T[ACGT]AGG
ApaLI 21 GTGCAC G^TGCAC
ApoI 1644 AAATTT [GA]^AATT[CT]
Asp700I 404 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2040 GGATCC G^GATCC
BbeI 1404 GGCGCC GGCGC^C
BciVI 1743 GGATAC GTATCC(6/5)
BfrI 2004 CTTAAG C^TTAAG
BglI 1772 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1529 AGATCT A^GATCT
BseAI 502 TCCGGA T^CCGGA
BseRI 1200 GAGGAG GAGGAG(10/8)
BsmBI 506 GAGACG CGTCTC(1/5)
BsmI 148 GAATGC GAATGC(1/-1)
BspEI 502 TCCGGA T^CCGGA
BspHI 1138 TCATGA T^CATGA
BsrBI 1812 CCGCTC CCGCTC(-3/-3)
BsrFI 343 GCCGGT [GA]^CCGG[CT]
BssSI 615 CACGAG CACGAG(-5/-1)
BstAPI 1085 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 193 GGTTACC G^GT[ACGT]ACC
BstPI 193 GGTTACC G^GT[ACGT]ACC
Bsu36I 1884 CCTTAGG CC^T[ACGT]AGG
Cfr10I 343 GCCGGT [GA]^CCGG[CT]
DraIII 786 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1884 CCTTAGG CC^T[ACGT]AGG
EcoNI 881 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 193 GGTTACC G^GT[ACGT]ACC
HincII 903 GTCAAC GT[CT]^[GA]AC
HindII 903 GTCAAC GT[CT]^[GA]AC
KasI 1404 GGCGCC G^GCGCC
MroI 502 TCCGGA T^CCGGA
NarI 1404 GGCGCC GG^CGCC
PpuMI 1665 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1138 TCATGA T^CATGA
SfiI 1771 GGCCCCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1404 GGCGCC GGC^GCC
SmaI 1445 CCCGGG CCC^GGG
SphI 1123 GCATGC GCATG^C
XmaCI 1445 CCCGGG C^CCGGG
XmaI 1445 CCCGGG C^CCGGG
XmnI 404 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AviII 835 TGCGCA TGC^GCA
1120 TGCGCA
BbrPI 209 CACGTG CAC^GTG
1095 CACGTG
BbsI 225 GAAGAC GAAGAC(2/6)
960 GTCTTC
BbvCI 2192 GCTGAGG CCTCAGC(-5/-2)
2327 GCTGAGG
BpuAI 225 GAAGAC GAAGAC(2/6)
960 GTCTTC
BsaAI 209 CACGTG [CT]AC^GT[GA]
1095 CACGTG
BsaWI 502 TCCGGA [AT]^CCGG[AT]
2010 TCCGGT
FspI 835 TGCGCA TGC^GCA
1120 TGCGCA
HaeII 284 GGCGCT [GA]GCGC^[CT]
1404 GGCGCC
MaeII 210 ACGT A^CGT
1096 ACGT
MseI 1627 TTAA T^TAA
2005 TTAA
NspI 910 ACATGC [GA]CATG^[CT]
1123 GCATGC
PflMI 785 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1540 CCAGCAGCTGG
PmaCI 209 CACGTG CAC^GTG
1095 CACGTG
PmlI 209 CACGTG CAC^GTG
1095 CACGTG
SacI 1797 GAGCTC GAGCT^C
2127 GAGCTC
SfaNI 1550 GATGC GCATC(5/9)
1764 GATGC
SmlI 2004 CTTAAG C^T[CT][GA]AG
2210 CTTGAG
TaqI 524 TCGA T^CGA
2225 TCGA
Tru9I 1627 TTAA T^TAA
2005 TTAA
Tsp509I 1625 AATT ^AATT
1645 AATT
TthHB8I 524 TCGA T^CGA
2225 TCGA
Van91I 785 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1540 CCAGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
Acc65I AciI AcyI AfaI AluI
AlwI AlwNI ApaI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI StuI
StyI TfiI TseI Tsp45I TspRI
XcmI XhoII