##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI AspEI AspI AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BseRI BsiWI BsmI
BspDI BspHI BspLU11I BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DpnII
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI Ksp632I KspI MamI MboI
MfeI MflI MluI MluNI MscI
MunI NdeI NdeII NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
Sau3AI SbfI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp509I
Tth111I XbaI XcmI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 290 GGTACC G^GTACC
AccIII 538 TCCGGA T^CCGGA
AflIII 591 ACGTGT A^C[GA][CT]GT
ApaI 974 GGGCCC GGGCC^C
Asp718I 290 GGTACC G^GTACC
AviII 299 TGCGCA TGC^GCA
BfaI 327 CTAG C^TAG
BlpI 832 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 832 GCTGAGC GC^T[ACGT]AGC
BseAI 538 TCCGGA T^CCGGA
BsgI 1205 CTGCAC GTGCAG(16/14)
BsmBI 689 GAGACG CGTCTC(1/5)
Bsp1407I 1366 TGTACA T^GTACA
BspEI 538 TCCGGA T^CCGGA
BspMI 380 ACCTGC ACCTGC(4/8)
BsrBI 815 GAGCGG CCGCTC(-3/-3)
BsrGI 1366 TGTACA T^GTACA
BssSI 26 CTCGTG CACGAG(-5/-1)
BstXI 678 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 832 GCTGAGC GC^T[ACGT]AGC
FspI 299 TGCGCA TGC^GCA
HincII 1262 GTCAAC GT[CT]^[GA]AC
HindII 1262 GTCAAC GT[CT]^[GA]AC
KpnI 290 GGTACC GGTAC^C
MaeI 327 CTAG C^TAG
MroI 538 TCCGGA T^CCGGA
MseI 1387 TTAA T^TAA
MslI 379 CACCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 125 GCCGGC GCC^GGC
NcoI 111 CCATGG C^CATGG
NgoMIV 125 GCCGGC G^CCGGC
NotI 886 GCGGCCGC GC^GGCCGC
NspI 771 ACATGC [GA]CATG^[CT]
PflMI 631 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 966 AGTACT AGT^ACT
SexAI 333 ACCTGGT A^CC[AT]GGT
SspBI 1366 TGTACA T^GTACA
TfiI 542 GATTC G^A[AT]TC
Tru9I 1387 TTAA T^TAA
Tsp45I 522 GTGAC ^GT[GC]AC
Van91I 631 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwNI 175 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
632 CAGAACCTG
BbsI 38 GTCTTC GAAGAC(2/6)
1226 GTCTTC
BpuAI 38 GTCTTC GAAGAC(2/6)
1226 GTCTTC
BsaI 758 GAGACC GGTCTC(1/5)
1337 GGTCTC
BsaWI 279 TCCGGT [AT]^CCGG[AT]
538 TCCGGA
BstAPI 1096 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1343 GCACGGTCTGC
DraII 176 AGGGCCT [GA]G^G[ACGT]CC[CT]
1006 AGGCCCC
DsaI 111 CCATGG C^C[GA][CT]GG
513 CCACGG
EcoO109I 176 AGGGCCT [GA]G^G[ACGT]CC[CT]
1006 AGGCCCC
HphI 364 TCACC GGTGA(8/7)
378 TCACC
SacI 23 GAGCTC GAGCT^C
917 GAGCTC
SfaNI 883 GATGC GCATC(5/9)
318 GCATC
TaqI 432 TCGA T^CGA
1121 TCGA
TthHB8I 432 TCGA T^CGA
1121 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Asp700I AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI EaeI EagI EclXI
Eco52I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI KasI MaeII MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIII NlaIV
PleI PvuII RsaI Sau96I ScrFI
SfoI SmaI StyI TseI TspRI
XmaCI XmaI XmnI