##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbeI
BcgI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstYI Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII FauI FseI
FspI HaeII HgaI Hin1I KasI
KpnI KspI MflI MluI MluNI
MroI MscI MspA1I MvaI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
StuI TaqI Tth111I TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 2131 GTATAC GT^[AC][GT]AC
AciI 18 GCGG CCGC(-3/-1)
AhdI 233 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 233 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 31 GGCACC G^G[CT][GA]CC
BbrPI 1725 CACGTG CAC^GTG
BbsI 386 GTCTTC GAAGAC(2/6)
BbvCI 12 GCTGAGG CCTCAGC(-5/-2)
BciVI 742 GTATCC GTATCC(6/5)
BcnI 28 CCGGG CC^[GC]GG
BmyI 30 GGGCAC G[AGT]GC[ACT]^C
BpmI 1882 CTGGAG CTGGAG(16/14)
BpuAI 386 GTCTTC GAAGAC(2/6)
BsaBI 951 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 314 GTGCAG GTGCAG(16/14)
BsiYI 163 CCACCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 163 CCACCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 2421 GTCTC GTCTC(1/5)
Bsp1286I 30 GGGCAC G[AGT]GC[ACT]^C
BspLU11I 1938 ACATGT A^CATGT
BspMI 1354 ACCTGC ACCTGC(4/8)
BssKI 28 CCGGG ^CC[ACGT]GG
Bst1107I 2131 GTATAC GTA^TAC
BstZ17I 2131 GTATAC GTA^TAC
Cac8I 2173 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 40 GCGC GCG^C
Eam1105I 233 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 633 GAATTC G^AATTC
EcoRV 1070 GATATC GAT^ATC
EcoT22I 1169 ATGCAT ATGCA^T
HapII 28 CCGG C^CGG
HhaI 40 GCGC GCG^C
HinP1I 40 GCGC G^CGC
HpaII 28 CCGG C^CGG
MaeIII 2480 GTGAC ^GT[ACGT]AC
MamI 951 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1124 CAATTG C^AATTG
MspI 28 CCGG C^CGG
MunI 1124 CAATTG C^AATTG
NciI 28 CCGGG CC^[GC]GG
NsiI 1169 ATGCAT ATGCA^T
PleI 1597 GACTC GAGTC(4/5)
PmaCI 1725 CACGTG CAC^GTG
PmlI 1725 CACGTG CAC^GTG
ScrFI 28 CCGGG CC^[ACGT]GG
SnaBI 1092 TACGTA TAC^GTA
SwaI 1956 ATTTAAAT ATTT^AAAT
Tsp45I 2480 GTGAC ^GT[GC]AC
XbaI 2411 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 1726 ACGTGT A^C[GA][CT]GT
1938 ACATGT
AlwI 454 GGATC GGATC(4/5)
950 GGATC
AlwNI 287 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
638 CAGTTTCTG
Asp700I 479 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1414 GAAGCTTTTC
BsaAI 1092 TACGTA [CT]AC^GT[GA]
1725 CACGTG
BsmFI 1 GGGAC GGGAC(10/14)
2550 GGGAC
BsmI 256 GAATGC GAATGC(1/-1)
1860 GAATGC
Bsp1407I 1935 TGTACA T^GTACA
2104 TGTACA
BsrGI 1935 TGTACA T^GTACA
2104 TGTACA
BstF5I 262 CATCC GGATG(2/0)
1309 GGATG
EarI 1599 CTCTTC CTCTTC(1/4)
619 GAAGAG
FokI 262 CATCC GGATG(9/13)
1309 GGATG
HincII 1112 GTTAAC GT[CT]^[GA]AC
2543 GTTAAC
HindII 1112 GTTAAC GT[CT]^[GA]AC
2543 GTTAAC
HindIII 1415 AAGCTT A^AGCTT
2171 AAGCTT
HpaI 1112 GTTAAC GTT^AAC
2543 GTTAAC
Ksp632I 1599 CTCTTC CTCTTC(1/4)
619 GAAGAG
MaeII 1093 ACGT A^CGT
1726 ACGT
NlaIV 31 GGCACC GG[ACGT]^[ACGT]CC
1755 GGTTCC
SspBI 1935 TGTACA T^GTACA
2104 TGTACA
XmnI 479 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1414 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI BbvI BclI BfaI BsaJI
BsrI DdeI DpnII DraI EcoT14I
FbaI Fnu4HI HaeIII HinfI HphI
ItaI MaeI MboI MboII MnlI
MseI MslI MwoI NdeII NlaIII
NspI PshBI RsaI Sau3AI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp509I TspRI