##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AccII AccIII AclI AcsI AflIII
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI AviII AvrII BamHI
BbrPI BcgI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI EcoO65I EcoRI
EcoT22I FseI FspI HgaI HincII
HindII HindIII HpaI KspI MamI
MluI MroI MvnI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SspI SwaI Van91I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 1094 GACGTC GACGT^C
AflII 1744 CTTAAG C^TTAAG
AhdI 1543 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1419 CCTTAGG CC^T[ACGT]AGG
Aor51HI 281 AGCGCT AGC^GCT
Asp700I 1161 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1543 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1310 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1619 TGGCCA TGG^CCA
BbsI 559 GTCTTC GAAGAC(2/6)
BbvCI 1714 GCTGAGG CCTCAGC(-5/-2)
BclI 1394 TGATCA T^GATCA
BfrI 1744 CTTAAG C^TTAAG
BglII 1673 AGATCT A^GATCT
BpuAI 559 GTCTTC GAAGAC(2/6)
BseRI 1594 CTCCTC GAGGAG(10/8)
BsgI 990 GTGCAG GTGCAG(16/14)
BsiEI 75 CGGCCG CG[GA][CT]^CG
BsmFI 827 GGGAC GGGAC(10/14)
BspHI 788 TCATGA T^CATGA
BsrBI 108 GAGCGG CCGCTC(-3/-3)
BstXI 1233 CCATGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1673 AGATCT [GA]^GATC[CT]
Bsu36I 1419 CCTTAGG CC^T[ACGT]AGG
DraI 1775 TTTAAA TTT^AAA
DraIII 1479 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 143 GACTGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 75 CGGCCG C^GGCCG
Eam1105I 1543 GACTAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1629 CTCTTC CTCTTC(1/4)
EclXI 75 CGGCCG C^GGCCG
Eco47III 281 AGCGCT AGC^GCT
Eco52I 75 CGGCCG C^GGCCG
Eco81I 1419 CCTTAGG CC^T[ACGT]AGG
EcoRV 619 GATATC GAT^ATC
FbaI 1394 TGATCA T^GATCA
Ksp632I 1629 CTCTTC CTCTTC(1/4)
MfeI 738 CAATTG C^AATTG
MflI 1673 AGATCT [GA]^GATC[CT]
MluNI 1619 TGGCCA TGG^CCA
MscI 1619 TGGCCA TGG^CCA
MslI 1234 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 738 CAATTG C^AATTG
NheI 1559 GCTAGC G^CTAGC
PaeR7I 1327 CTCGAG C^TCGAG
PflFI 1310 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 827 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 1667 CAGCTG CAG^CTG
RcaI 788 TCATGA T^CATGA
SpeI 1660 ACTAGT A^CTAGT
SphI 1032 GCATGC GCATG^C
StuI 1705 AGGCCT AGG^CCT
Tth111I 1310 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 508 CCACGACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1327 CTCGAG C^TCGAG
XhoII 1673 AGATCT [GA]^GATC[CT]
XmnI 1161 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 596 GGTACC G^GTACC
1425 GGTACC
AlwNI 684 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
1283 CAGCCTCTG
ApaI 70 GGGCCC GGGCC^C
198 GGGCCC
Asp718I 596 GGTACC G^GTACC
1425 GGTACC
AvaI 1202 CTCGGG C^[CT]CG[GA]G
1327 CTCGAG
BbeI 82 GGCGCC GGCGC^C
245 GGCGCC
BciVI 1179 GGATAC GTATCC(6/5)
1602 GGATAC
BpmI 1597 CTCCAG CTGGAG(16/14)
1612 CTGGAG
BsmBI 630 CGTCTC CGTCTC(1/5)
1092 GAGACG
BsoBI 1202 CTCGGG C^[CT]CG[GA]G
1327 CTCGAG
BstAPI 296 GCAAGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
683 GCAGCATCTGC
DraII 827 GGGACCT [GA]G^G[ACGT]CC[CT]
1361 AGGGCCT
DsaI 48 CCATGG C^C[GA][CT]GG
1233 CCATGG
EaeI 75 CGGCCG [CT]^GGCC[GA]
1619 TGGCCA
EcoNI 1415 CCTGCCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1593 CCTCCTCCAGG
EcoO109I 827 GGGACCT [GA]G^G[ACGT]CC[CT]
1361 AGGGCCT
KasI 82 GGCGCC G^GCGCC
245 GGCGCC
KpnI 596 GGTACC GGTAC^C
1425 GGTACC
MaeII 392 ACGT A^CGT
1095 ACGT
MseI 1745 TTAA T^TAA
1776 TTAA
NarI 82 GGCGCC GG^CGCC
245 GGCGCC
NcoI 48 CCATGG C^CATGG
1233 CCATGG
NspI 1032 GCATGC [GA]CATG^[CT]
1736 ACATGC
SfoI 82 GGCGCC GGC^GCC
245 GGCGCC
SmlI 1327 CTCGAG C^T[CT][GA]AG
1744 CTTAAG
Tru9I 1745 TTAA T^TAA
1776 TTAA
Tsp509I 739 AATT ^AATT
1440 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AspHI AvaII BanI BanII
BbvI BcnI BfaI BmyI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I