##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BpmI BsaAI BsaBI
BsgI BsiEI BsiWI BsmBI BspDI
BspHI BspLU11I BsrDI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
DraII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV FseI FspI
HindIII Ksp632I KspI MaeII MamI
MluI NaeI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 381 GGTACC G^GTACC
AccIII 825 TCCGGA T^CCGGA
AgeI 870 ACCGGT A^CCGGT
AlwNI 1724 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1476 ATTAAT AT^TAAT
AsnI 1476 ATTAAT AT^TAAT
Asp718I 381 GGTACC G^GTACC
AvaI 172 CCCGAG C^[CT]CG[GA]G
BalI 299 TGGCCA TGG^CCA
BlpI 412 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 412 GCTAAGC GC^T[ACGT]AGC
BseAI 825 TCCGGA T^CCGGA
BsmI 541 GAATGC GAATGC(1/-1)
BsoBI 172 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1689 TGTACA T^GTACA
BspEI 825 TCCGGA T^CCGGA
BspMI 2160 GCAGGT ACCTGC(4/8)
BsrFI 870 ACCGGT [GA]^CCGG[CT]
BsrGI 1689 TGTACA T^GTACA
BstAPI 550 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 885 GGTCACC G^GT[ACGT]ACC
BstPI 885 GGTCACC G^GT[ACGT]ACC
CelII 412 GCTAAGC GC^T[ACGT]AGC
Cfr10I 870 ACCGGT [GA]^CCGG[CT]
CpoI 867 CGGACCG CG^G[AT]CCG
DraIII 1335 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 885 GGTCACC G^GT[ACGT]ACC
EcoT22I 1973 ATGCAT ATGCA^T
HgaI 496 GACGC GACGC(5/10)
KpnI 381 GGTACC GGTAC^C
MfeI 1944 CAATTG C^AATTG
MluNI 299 TGGCCA TGG^CCA
MroI 825 TCCGGA T^CCGGA
MscI 299 TGGCCA TGG^CCA
MslI 210 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1944 CAATTG C^AATTG
NsiI 1973 ATGCAT ATGCA^T
PinAI 870 ACCGGT A^CCGGT
PshBI 1476 ATTAAT AT^TAAT
PstI 436 CTGCAG CTGCA^G
RsrII 867 CGGACCG CG^G[AT]CCG
SacI 164 GAGCTC GAGCT^C
SmlI 249 CTTGAG C^T[CT][GA]AG
SspBI 1689 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AspHI 164 GAGCTC G[AT]GC[AT]^C
874 GTGCTC
AvaII 698 GGACC G^G[AT]CC
868 GGACC
BclI 1626 TGATCA T^GATCA
1923 TGATCA
BsiHKAI 164 GAGCTC G[AT]GC[AT]^C
874 GTGCTC
BstF5I 823 CATCC GGATG(2/0)
11 GGATG
BstXI 209 CCATCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2181 CCACTGAACTGG
EaeI 299 TGGCCA [CT]^GGCC[GA]
753 CGGCCA
FbaI 1626 TGATCA T^GATCA
1923 TGATCA
FokI 823 CATCC GGATG(9/13)
11 GGATG
PflMI 291 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1723 CCAGCTGCTGG
PvuII 1112 CAGCTG CAG^CTG
1724 CAGCTG
SpeI 1280 ACTAGT A^CTAGT
1651 ACTAGT
SspI 1525 AATATT AAT^ATT
1557 AATATT
Tsp45I 886 GTCAC ^GT[GC]AC
2220 GTGAC
Van91I 291 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1723 CCAGCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI BanI BanII
BbeI BbsI BbvI BfaI BmyI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrI BssKI BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaI HpaII HphI
ItaI KasI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII