##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AviII BbsI
BcgI BciVI BclI BfrI BlpI
Bpu1102I BpuAI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmI BspEI BspHI
BspLU11I BsrDI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MfeI MluI MroI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 703 GACGTC GACGT^C
AflIII 1416 ACACGT A^C[GA][CT]GT
AspI 874 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1615 CCTAGG C^CTAGG
BalI 1175 TGGCCA TGG^CCA
BamHI 1836 GGATCC G^GATCC
BbrPI 1417 CACGTG CAC^GTG
BbvCI 403 CCTCAGC CCTCAGC(-5/-2)
BglII 587 AGATCT A^GATCT
BlnI 1615 CCTAGG C^CTAGG
BsaAI 1417 CACGTG [CT]AC^GT[GA]
BsaBI 864 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1361 GTGCAG GTGCAG(16/14)
Bsp1407I 996 TGTACA T^GTACA
BspDI 1333 ATCGAT AT^CGAT
BsrBI 601 CCGCTC CCGCTC(-3/-3)
BsrGI 996 TGTACA T^GTACA
BstXI 869 CCATCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1333 ATCGAT AT^CGAT
MamI 864 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1175 TGGCCA TGG^CCA
MscI 1175 TGGCCA TGG^CCA
NaeI 398 GCCGGC GCC^GGC
NcoI 410 CCATGG C^CATGG
NgoMIV 398 GCCGGC G^CCGGC
NheI 475 GCTAGC G^CTAGC
NspI 649 ACATGC [GA]CATG^[CT]
PflFI 874 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 231 GAGTC GAGTC(4/5)
PmaCI 1417 CACGTG CAC^GTG
PmlI 1417 CACGTG CAC^GTG
PstI 903 CTGCAG CTGCA^G
PvuII 1249 CAGCTG CAG^CTG
SapI 603 GCTCTTC GCTCTTC(1/4)
SexAI 875 ACCTGGT A^CC[AT]GGT
SmlI 1663 CTCAAG C^T[CT][GA]AG
SspBI 996 TGTACA T^GTACA
StuI 1679 AGGCCT AGG^CCT
Tsp509I 1894 AATT ^AATT
Tth111I 874 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AhdI 17 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1008 GACCCCAGGTC
AspEI 17 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1008 GACCCCAGGTC
BanI 139 GGCGCC G^G[CT][GA]CC
248 GGCGCC
BbeI 139 GGCGCC GGCGC^C
248 GGCGCC
BglI 166 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1373 GCCCAGAGGGC
BsaI 974 GAGACC GGTCTC(1/5)
1503 GAGACC
BssHII 282 GCGCGC G^CGCGC
284 GCGCGC
Eam1105I 17 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1008 GACCCCAGGTC
HaeII 139 GGCGCC [GA]GCGC^[CT]
248 GGCGCC
HincII 314 GTCAAC GT[CT]^[GA]AC
1285 GTCAAC
HindII 314 GTCAAC GT[CT]^[GA]AC
1285 GTCAAC
KasI 139 GGCGCC G^GCGCC
248 GGCGCC
KspI 321 CCGCGG CCGC^GG
363 CCGCGG
MaeII 704 ACGT A^CGT
1418 ACGT
MslI 819 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1083 CACCAGCGTG
NarI 139 GGCGCC GG^CGCC
248 GGCGCC
SacII 321 CCGCGG CCGC^GG
363 CCGCGG
SfcI 903 CTGCAG C^T[GA][CT]AG
1483 CTATAG
SfoI 139 GGCGCC GGC^GCC
248 GGCGCC
SmaI 30 CCCGGG CCC^GGG
345 CCCGGG
XmaCI 30 CCCGGG C^CCGGG
345 CCCGGG
XmaI 30 CCCGGG C^CCGGG
345 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AvaI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII