##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AviII BbeI BbrPI
BciVI BclI BfrI BglII BlpI
Bpu1102I BsaAI BsaI BsiWI BsmI
BspDI BspLU11I BspMI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII ClaI CpoI DraI Eam1105I
Eco47III EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 195 GACGTC GACGT^C
AccI 3 GTAGAC GT^[AC][GT]AC
AccII 119 CGCG CG^CG
AccIII 1898 TCCGGA T^CCGGA
AcyI 195 GACGTC G[GA]^CG[CT]C
Alw44I 20 GTGCAC G^TGCAC
AocI 53 CCTGAGG CC^T[ACGT]AGG
ApaLI 20 GTGCAC G^TGCAC
Asp700I 358 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 752 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 41 CCTAGG C^CTAGG
BamHI 1895 GGATCC G^GATCC
BbvCI 1727 GCTGAGG CCTCAGC(-5/-2)
BcgI 109 GCAGCTGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 42 CTAG C^TAG
BlnI 41 CCTAGG C^CTAGG
BsaBI 1396 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 195 GACGTC G[GA]^CG[CT]C
BsaWI 1898 TCCGGA [AT]^CCGG[AT]
BseAI 1898 TCCGGA T^CCGGA
BseRI 1843 CTCCTC GAGGAG(10/8)
BsgI 1580 CTGCAC GTGCAG(16/14)
BsiEI 889 CGGCCG CG[GA][CT]^CG
BsmBI 197 CGTCTC CGTCTC(1/5)
BspEI 1898 TCCGGA T^CCGGA
BspHI 1101 TCATGA T^CATGA
BsrFI 795 GCCGGC [GA]^CCGG[CT]
BstEII 1620 GGTGACC G^GT[ACGT]ACC
BstPI 1620 GGTGACC G^GT[ACGT]ACC
BstUI 119 CGCG CG^CG
Bsu36I 53 CCTGAGG CC^T[ACGT]AGG
Cfr10I 795 GCCGGC [GA]^CCGG[CT]
DraIII 1130 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 749 GACGACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 812 CCGTGG C^C[GA][CT]GG
EagI 889 CGGCCG C^GGCCG
EarI 1091 CTCTTC CTCTTC(1/4)
EclXI 889 CGGCCG C^GGCCG
Eco52I 889 CGGCCG C^GGCCG
Eco81I 53 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1620 GGTGACC G^GT[ACGT]ACC
Hin1I 195 GACGTC G[GA]^CG[CT]C
HindIII 359 AAGCTT A^AGCTT
Ksp632I 1091 CTCTTC CTCTTC(1/4)
MaeI 42 CTAG C^TAG
MamI 1396 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1898 TCCGGA T^CCGGA
MvnI 119 CGCG CG^CG
NaeI 795 GCCGGC GCC^GGC
NgoMIV 795 GCCGGC G^CCGGC
PflFI 752 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1101 TCATGA T^CATGA
SmaI 163 CCCGGG CCC^GGG
TfiI 1160 GAATC G^A[AT]TC
Tth111I 752 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 163 CCCGGG C^CCGGG
XmaI 163 CCCGGG C^CCGGG
XmnI 358 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 1939 AAATTT [GA]^AATT[CT]
1990 AAATTT
AlwNI 1022 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1626 CAGACACTG
ApaI 166 GGGCCC GGGCC^C
1766 GGGCCC
ApoI 1939 AAATTT [GA]^AATT[CT]
1990 AAATTT
AvaI 80 CCCGAG C^[CT]CG[GA]G
163 CCCGGG
BbsI 1169 GAAGAC GAAGAC(2/6)
906 GTCTTC
BpuAI 1169 GAAGAC GAAGAC(2/6)
906 GTCTTC
BsoBI 80 CCCGAG C^[CT]CG[GA]G
163 CCCGGG
Bsp1407I 1881 TGTACA T^GTACA
1915 TGTACA
BsrDI 849 CATTGC GCAATG(2/0)
951 GCAATG
BsrGI 1881 TGTACA T^GTACA
1915 TGTACA
CfoI 177 GCGC GCG^C
1213 GCGC
HaeII 176 GGCGCT [GA]GCGC^[CT]
1212 AGCGCC
HgaI 412 GACGC GACGC(5/10)
913 GCGTC
HhaI 177 GCGC GCG^C
1213 GCGC
HinP1I 177 GCGC G^CGC
1213 GCGC
MslI 760 CACCCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1637 CACTGCAGTG
PflMI 1129 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1593 CCAAGGACTGG
SacI 1793 GAGCTC GAGCT^C
1909 GAGCTC
SfaNI 1188 GCATC GCATC(5/9)
1428 GCATC
SspBI 1881 TGTACA T^GTACA
1915 TGTACA
StuI 1755 AGGCCT AGG^CCT
1839 AGGCCT
Van91I 1129 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1593 CCAAGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BalI BanI BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII