##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AviII AvrII BalI BanI
BbeI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I CelII ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 1951 CGCG CG^CG
AccIII 444 TCCGGA T^CCGGA
AocI 296 CCTCAGG CC^T[ACGT]AGG
AseI 2535 ATTAAT AT^TAAT
AsnI 2535 ATTAAT AT^TAAT
AspI 3 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 92 CCCGGG C^[CT]CG[GA]G
BamHI 1089 GGATCC G^GATCC
BbrPI 1597 CACGTG CAC^GTG
BclI 556 TGATCA T^GATCA
BsaAI 1597 CACGTG [CT]AC^GT[GA]
BseAI 444 TCCGGA T^CCGGA
BseRI 743 GAGGAG GAGGAG(10/8)
BsgI 227 CTGCAC GTGCAG(16/14)
BsiEI 1572 CGACCG CG[GA][CT]^CG
BsoBI 92 CCCGGG C^[CT]CG[GA]G
BspEI 444 TCCGGA T^CCGGA
BspHI 2087 TCATGA T^CATGA
BspMI 469 ACCTGC ACCTGC(4/8)
BsrDI 1203 GCAATG GCAATG(2/0)
BsrFI 2308 ACCGGC [GA]^CCGG[CT]
BstEII 412 GGTAACC G^GT[ACGT]ACC
BstPI 412 GGTAACC G^GT[ACGT]ACC
BstUI 1951 CGCG CG^CG
BstYI 1089 GGATCC [GA]^GATC[CT]
Bsu36I 296 CCTCAGG CC^T[ACGT]AGG
CfoI 383 GCGC GCG^C
Cfr10I 2308 ACCGGC [GA]^CCGG[CT]
Eco81I 296 CCTCAGG CC^T[ACGT]AGG
EcoO65I 412 GGTAACC G^GT[ACGT]ACC
FbaI 556 TGATCA T^GATCA
HhaI 383 GCGC GCG^C
HinP1I 383 GCGC G^CGC
MaeII 1598 ACGT A^CGT
MflI 1089 GGATCC [GA]^GATC[CT]
MroI 444 TCCGGA T^CCGGA
MvnI 1951 CGCG CG^CG
NcoI 261 CCATGG C^CATGG
NspI 2072 ACATGC [GA]CATG^[CT]
PflFI 3 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1597 CACGTG CAC^GTG
PmlI 1597 CACGTG CAC^GTG
PshBI 2535 ATTAAT AT^TAAT
RcaI 2087 TCATGA T^CATGA
SapI 317 GCTCTTC GCTCTTC(1/4)
SexAI 2223 ACCTGGT A^CC[AT]GGT
SmaI 92 CCCGGG CCC^GGG
Tth111I 3 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1089 GGATCC [GA]^GATC[CT]
XmaCI 92 CCCGGG C^CCGGG
XmaI 92 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 436 GTAC GT^AC
1480 GTAC
BbsI 572 GAAGAC GAAGAC(2/6)
1132 GAAGAC
BpuAI 572 GAAGAC GAAGAC(2/6)
1132 GAAGAC
BsaWI 31 TCCGGT [AT]^CCGG[AT]
444 TCCGGA
DraI 203 TTTAAA TTT^AAA
638 TTTAAA
DsaI 261 CCATGG C^C[GA][CT]GG
843 CCGTGG
EcoT14I 261 CCATGG C^C[AT][AT]GG
1891 CCAAGG
FauI 210 CCCGC CCCGC(4/6)
217 CCCGC
HgaI 1981 GACGC GACGC(5/10)
1778 GCGTC
RsaI 436 GTAC GT^AC
1480 GTAC
SmlI 632 CTCAAG C^T[CT][GA]AG
1641 CTCAAG
StyI 261 CCATGG C^C[AT][AT]GG
1891 CCAAGG
TaqI 892 TCGA T^CGA
1926 TCGA
Tsp45I 1 GTGAC ^GT[GC]AC
734 GTGAC
TthHB8I 892 TCGA T^CGA
1926 TCGA
XcmI 1891 CCAAGGTGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2214 CCAAGAGAAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI Asp700I AspHI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoNI EcoO109I
EcoRI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI TfiI Tru9I TseI
Tsp509I TspRI Van91I XmnI