##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeIII MamI MfeI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp45I Tth111I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 229 GTAGAC GT^[AC][GT]AC
AcsI 499 GAATTC [GA]^AATT[CT]
AlwNI 721 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 499 GAATTC [GA]^AATT[CT]
BalI 540 TGGCCA TGG^CCA
BamHI 248 GGATCC G^GATCC
BpmI 439 CTCCAG CTGGAG(16/14)
BsaAI 532 TACGTG [CT]AC^GT[GA]
BsaWI 480 ACCGGA [AT]^CCGG[AT]
BsmAI 664 GAGAC GTCTC(1/5)
BsmBI 664 GAGACG CGTCTC(1/5)
BspDI 821 ATCGAT AT^CGAT
BspMI 349 ACCTGC ACCTGC(4/8)
BsrDI 587 CATTGC GCAATG(2/0)
BsrI 253 CCAGT ACTGG(1/-1)
BstF5I 537 GGATG GGATG(2/0)
ClaI 821 ATCGAT AT^CGAT
EcoRI 499 GAATTC G^AATTC
EcoT22I 4 ATGCAT ATGCA^T
FokI 537 GGATG GGATG(9/13)
HgaI 181 GACGC GACGC(5/10)
MluNI 540 TGGCCA TGG^CCA
MscI 540 TGGCCA TGG^CCA
MspA1I 244 CAGCGG C[AC]G^C[GT]G
NsiI 4 ATGCAT ATGCA^T
PstI 58 CTGCAG CTGCA^G
SexAI 76 ACCTGGT A^CC[AT]GGT
SfaNI 3 GATGC GCATC(5/9)
SfcI 58 CTGCAG C^T[GA][CT]AG
TaqI 822 TCGA T^CGA
TfiI 775 GAATC G^A[AT]TC
Tsp509I 500 AATT ^AATT
TthHB8I 822 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AluI 485 AGCT AG^CT
810 AGCT
ApaI 603 GGGCCC GGGCC^C
739 GGGCCC
AvaII 309 GGACC G^G[AT]CC
576 GGACC
BanI 73 GGCACC G^G[CT][GA]CC
627 GGTGCC
BanII 603 GGGCCC G[GA]GC[CT]^C
739 GGGCCC
BbsI 370 GAAGAC GAAGAC(2/6)
409 GAAGAC
BfaI 421 CTAG C^TAG
745 CTAG
BglI 19 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
331 GCCTCCCTGGC
BpuAI 370 GAAGAC GAAGAC(2/6)
409 GAAGAC
BstYI 248 GGATCC [GA]^GATC[CT]
381 GGATCT
DdeI 237 CTCAG C^T[ACGT]AG
771 CTGAG
DraIII 75 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
301 CACGGGGTG
EaeI 14 TGGCCG [CT]^GGCC[GA]
540 TGGCCA
EcoNI 435 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
757 CCTTCCCCAGG
EcoT14I 23 CCATGG C^C[AT][AT]GG
592 CCATGG
FauI 169 GCGGG CCCGC(4/6)
184 GCGGG
HaeII 123 GGCGCT [GA]GCGC^[CT]
296 AGCGCC
MaeI 421 CTAG C^TAG
745 CTAG
MflI 248 GGATCC [GA]^GATC[CT]
381 GGATCT
NcoI 23 CCATGG C^CATGG
592 CCATGG
NspI 6 GCATGT [GA]CATG^[CT]
416 ACATGC
StyI 23 CCATGG C^C[AT][AT]GG
592 CCATGG
TspRI 254 CAGTG CA[GC]TG[ACGT][ACGT]^
559 CACTG
XhoII 248 GGATCC [GA]^GATC[CT]
381 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AlwI AvaI BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrBI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DpnII DraII DsaI EcoO109I
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MnlI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
Sau3AI Sau96I ScrFI SmaI TseI
Van91I XmaCI XmaI