##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AhdI AlwNI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BbrPI BbsI BbvCI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraIII
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflIII 135 ACGTGT A^C[GA][CT]GT
AgeI 650 ACCGGT A^CCGGT
Alw44I 321 GTGCAC G^TGCAC
ApaI 1010 GGGCCC GGGCC^C
ApaLI 321 GTGCAC G^TGCAC
AspI 877 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 392 TGGCCA TGG^CCA
BbeI 984 GGCGCC GGCGC^C
BcgI 699 CGATGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 14 CTAG C^TAG
BsaAI 573 CACGTA [CT]AC^GT[GA]
BsaI 310 GAGACC GGTCTC(1/5)
BsgI 211 CTGCAC GTGCAG(16/14)
BsiEI 917 CGGCCG CG[GA][CT]^CG
BsmI 927 GAATGC GAATGC(1/-1)
BsrDI 705 CATTGC GCAATG(2/0)
BsrFI 650 ACCGGT [GA]^CCGG[CT]
BssSI 254 CTCGTG CACGAG(-5/-1)
BstXI 956 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 985 GCGC GCG^C
Cfr10I 650 ACCGGT [GA]^CCGG[CT]
DrdI 874 GACGACAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 917 CGGCCG C^GGCCG
EclXI 917 CGGCCG C^GGCCG
Eco52I 917 CGGCCG C^GGCCG
EcoRV 97 GATATC GAT^ATC
HaeII 984 GGCGCC [GA]GCGC^[CT]
HhaI 985 GCGC GCG^C
HinP1I 985 GCGC G^CGC
HphI 993 GGTGA GGTGA(8/7)
KasI 984 GGCGCC G^GCGCC
MaeI 14 CTAG C^TAG
MluNI 392 TGGCCA TGG^CCA
MscI 392 TGGCCA TGG^CCA
NarI 984 GGCGCC GG^CGCC
PflFI 877 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 650 ACCGGT A^CCGGT
SapI 851 GCTCTTC GCTCTTC(1/4)
SfiI 909 GGCCCACGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 984 GGCGCC GGC^GCC
SmlI 715 CTCAAG C^T[CT][GA]AG
TaqI 821 TCGA T^CGA
Tth111I 877 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 821 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 34 TCCGGA T^CCGGA
54 TCCGGA
AcyI 505 GACGCC G[GA]^CG[CT]C
984 GGCGCC
BanI 229 GGCACC G^G[CT][GA]CC
984 GGCGCC
BsaHI 505 GACGCC G[GA]^CG[CT]C
984 GGCGCC
BseAI 34 TCCGGA T^CCGGA
54 TCCGGA
BseRI 155 GAGGAG GAGGAG(10/8)
823 GAGGAG
BsmAI 310 GAGAC GTCTC(1/5)
401 GTCTC
BsmFI 181 GGGAC GGGAC(10/14)
747 GTCCC
BspEI 34 TCCGGA T^CCGGA
54 TCCGGA
BspMI 722 ACCTGC ACCTGC(4/8)
1106 ACCTGC
BsrI 348 CCAGT ACTGG(1/-1)
804 CCAGT
BstF5I 858 CATCC GGATG(2/0)
89 GGATG
DsaI 266 CCATGG C^C[GA][CT]GG
589 CCATGG
EaeI 392 TGGCCA [CT]^GGCC[GA]
917 CGGCCG
FokI 858 CATCC GGATG(9/13)
89 GGATG
Hin1I 505 GACGCC G[GA]^CG[CT]C
984 GGCGCC
MaeIII 218 GTGAC ^GT[ACGT]AC
359 GTGAC
MroI 34 TCCGGA T^CCGGA
54 TCCGGA
MslI 411 CATCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
543 CATGGAGATG
NcoI 266 CCATGG C^CATGG
589 CCATGG
NspI 369 GCATGT [GA]CATG^[CT]
669 GCATGT
PvuII 763 CAGCTG CAG^CTG
1126 CAGCTG
SacI 160 GAGCTC GAGCT^C
271 GAGCTC
ScaI 692 AGTACT AGT^ACT
827 AGTACT
StuI 887 AGGCCT AGG^CCT
1175 AGGCCT
Tsp45I 218 GTGAC ^GT[GC]AC
359 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BamHI BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BsaWI BsiHKAI BsiYI BslI Bsp1286I
BssKI BstNI BstUI BstYI Cac8I
Cfr13I DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HapII HgaI HinfI HpaII ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI TspRI XhoII