##################################
# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AhdI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BseRI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspDI
BspEI BspHI BspMI BsrBI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV FauI FseI FspI
HaeIII HgaI Hin1I KasI KpnI
KspI MaeI MaeII MamI MluI
MluNI MroI MscI MslI MspA1I
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NspV PacI PaeR7I PflFI
PflMI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AflIII 676 ACATGT A^C[GA][CT]GT
AgeI 6 ACCGGT A^CCGGT
AluI 68 AGCT AG^CT
Aor51HI 12 AGCGCT AGC^GCT
AseI 180 ATTAAT AT^TAAT
AsnI 180 ATTAAT AT^TAAT
AvaII 938 GGTCC G^G[AT]CC
BbsI 472 GAAGAC GAAGAC(2/6)
BglII 135 AGATCT A^GATCT
BpuAI 472 GAAGAC GAAGAC(2/6)
BsaJI 359 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 6 ACCGGT [AT]^CCGG[AT]
BsgI 717 CTGCAC GTGCAG(16/14)
BsmI 288 GCATTC GAATGC(1/-1)
BspLU11I 676 ACATGT A^CATGT
BsrDI 313 GCAATG GCAATG(2/0)
BsrFI 6 ACCGGT [GA]^CCGG[CT]
BssKI 905 CCAGG ^CC[ACGT]GG
BstAPI 1015 GCACAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 308 GGATG GGATG(2/0)
BstNI 905 CCAGG CC^[AT]GG
BstYI 135 AGATCT [GA]^GATC[CT]
Cac8I 15 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 13 GCGC GCG^C
Cfr10I 6 ACCGGT [GA]^CCGG[CT]
Cfr13I 938 GGTCC G^G[ACGT]CC
Eco47III 12 AGCGCT AGC^GCT
EcoRI 57 GAATTC G^AATTC
EcoRII 905 CCAGG ^CC[AT]GG
EcoT14I 359 CCTTGG C^C[AT][AT]GG
EcoT22I 579 ATGCAT ATGCA^T
FokI 308 GGATG GGATG(9/13)
HaeII 12 AGCGCT [GA]GCGC^[CT]
HapII 7 CCGG C^CGG
HhaI 13 GCGC GCG^C
HinP1I 13 GCGC G^CGC
HindIII 67 AAGCTT A^AGCTT
HinfI 149 GAATC G^A[ACGT]TC
HpaI 371 GTTAAC GTT^AAC
HpaII 7 CCGG C^CGG
MfeI 211 CAATTG C^AATTG
MflI 135 AGATCT [GA]^GATC[CT]
MspI 7 CCGG C^CGG
MunI 211 CAATTG C^AATTG
MvaI 905 CCAGG CC^[AT]GG
NsiI 579 ATGCAT ATGCA^T
PinAI 6 ACCGGT A^CCGGT
PshBI 180 ATTAAT AT^TAAT
PstI 331 CTGCAG CTGCA^G
SapI 42 GAAGAGC GCTCTTC(1/4)
Sau96I 938 GGTCC G^G[ACGT]CC
ScrFI 905 CCAGG CC^[ACGT]GG
SfaNI 192 GATGC GCATC(5/9)
SfcI 331 CTGCAG C^T[GA][CT]AG
StyI 359 CCTTGG C^C[AT][AT]GG
TfiI 149 GAATC G^A[AT]TC
TspRI 266 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 135 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BbvI 19 GCAGC GCAGC(8/12)
330 GCTGC
BclI 397 TGATCA T^GATCA
666 TGATCA
DdeI 707 CTAAG C^T[ACGT]AG
730 CTTAG
DraI 776 TTTAAA TTT^AAA
979 TTTAAA
EarI 42 GAAGAG CTCTTC(1/4)
441 GAAGAG
FbaI 397 TGATCA T^GATCA
666 TGATCA
Fnu4HI 19 GCAGC GC^[ACGT]GC
330 GCTGC
HincII 371 GTTAAC GT[CT]^[GA]AC
855 GTCAAC
HindII 371 GTTAAC GT[CT]^[GA]AC
855 GTCAAC
HphI 93 GGTGA GGTGA(8/7)
420 GGTGA
ItaI 19 GCAGC GC^[ACGT]GC
330 GCTGC
Ksp632I 42 GAAGAG CTCTTC(1/4)
441 GAAGAG
MwoI 13 GCGCTTGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1015 GCACAATTTGC
TseI 19 GCAGC G^C[AT]GC
330 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI AfaI ApoI Bsp1407I BsrGI
DpnII MaeIII MboI MboII MnlI
MseI NdeII NlaIII NspI RsaI
Sau3AI SspBI Tru9I Tsp509I