##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI Asp700I Asp718I
AspEI AviII AvrII BalI BanI
BbeI BcgI BciVI BfaI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HindIII HpaI KasI KpnI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmeI PshAI Psp1406I
PstI PvuI RcaI RsrII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 990 GTCTAC GT^[AC][GT]AC
AflIII 606 ACGTGT A^C[GA][CT]GT
AseI 929 ATTAAT AT^TAAT
AsnI 929 ATTAAT AT^TAAT
AspI 984 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 338 GGATCC G^GATCC
BbrPI 605 CACGTG CAC^GTG
BbsI 9 GTCTTC GAAGAC(2/6)
BclI 869 TGATCA T^GATCA
BlpI 27 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 27 GCTGAGC GC^T[ACGT]AGC
BpuAI 9 GTCTTC GAAGAC(2/6)
BsaAI 605 CACGTG [CT]AC^GT[GA]
BseRI 752 CTCCTC GAGGAG(10/8)
BsgI 84 CTGCAC GTGCAG(16/14)
BsmFI 684 GGGAC GGGAC(10/14)
BsrDI 388 GCAATG GCAATG(2/0)
BsrFI 110 GCCGGT [GA]^CCGG[CT]
BstYI 338 GGATCC [GA]^GATC[CT]
CelII 27 GCTGAGC GC^T[ACGT]AGC
CfoI 57 GCGC GCG^C
Cfr10I 110 GCCGGT [GA]^CCGG[CT]
EarI 755 CTCTTC CTCTTC(1/4)
FbaI 869 TGATCA T^GATCA
HhaI 57 GCGC GCG^C
HinP1I 57 GCGC G^CGC
HincII 375 GTCAAC GT[CT]^[GA]AC
HindII 375 GTCAAC GT[CT]^[GA]AC
Ksp632I 755 CTCTTC CTCTTC(1/4)
KspI 69 CCGCGG CCGC^GG
MaeII 606 ACGT A^CGT
MflI 338 GGATCC [GA]^GATC[CT]
PflFI 984 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 605 CACGTG CAC^GTG
PmlI 605 CACGTG CAC^GTG
PpuMI 684 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 929 ATTAAT AT^TAAT
PvuII 654 CAGCTG CAG^CTG
SacII 69 CCGCGG CCGC^GG
SexAI 985 ACCAGGT A^CC[AT]GGT
SfcI 992 CTACAG C^T[GA][CT]AG
SmlI 140 CTTGAG C^T[CT][GA]AG
StuI 452 AGGCCT AGG^CCT
Tth111I 984 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 338 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 549 AAATTC [GA]^AATT[CT]
874 AAATTT
AcyI 230 GACGCC G[GA]^CG[CT]C
560 GGCGTC
AfaI 647 GTAC GT^AC
905 GTAC
AlwI 339 GATCC GGATC(4/5)
338 GGATC
ApaI 135 GGGCCC GGGCC^C
589 GGGCCC
ApoI 549 AAATTC [GA]^AATT[CT]
874 AAATTT
AspHI 30 GAGCTC G[AT]GC[AT]^C
582 GAGCTC
AvaI 14 CCCGAG C^[CT]CG[GA]G
431 CTCGGG
BbvCI 119 CCTCAGC CCTCAGC(-5/-2)
518 CCTCAGC
BglI 195 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
591 GCCCCGGTGGC
BpmI 532 CTGGAG CTGGAG(16/14)
699 CTGGAG
BsaHI 230 GACGCC G[GA]^CG[CT]C
560 GGCGTC
BsaI 535 GAGACC GGTCTC(1/5)
781 GAGACC
BsiHKAI 30 GAGCTC G[AT]GC[AT]^C
582 GAGCTC
BsmAI 535 GAGAC GTCTC(1/5)
781 GAGAC
BsoBI 14 CCCGAG C^[CT]CG[GA]G
431 CTCGGG
BstAPI 86 GCACGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
173 GCACCCGCTGC
BstXI 106 CCATGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
790 CCAGTCAGATGG
DsaI 69 CCGCGG C^C[GA][CT]GG
542 CCGTGG
FauI 54 CCCGC CCCGC(4/6)
176 CCCGC
Hin1I 230 GACGCC G[GA]^CG[CT]C
560 GGCGTC
HinfI 282 GATTC G^A[ACGT]TC
1018 GAATC
MboII 10 TCTTC GAAGA(8/7)
756 TCTTC
MslI 107 CATGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
459 CACCGCTATG
NspI 182 GCATGT [GA]CATG^[CT]
735 GCATGT
RsaI 647 GTAC GT^AC
905 GTAC
SacI 30 GAGCTC GAGCT^C
582 GAGCTC
TfiI 282 GATTC G^A[AT]TC
1018 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HpaII HphI ItaI MaeIII MboI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI