##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII ClaI DpnII
DraI DraIII Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
MaeI MamI MboI MfeI MflI
MluI MroI MslI MunI NarI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SacI
SalI Sau3AI ScaI SfiI SfoI
SfuI SmlI SnaBI SpeI SphI
SspBI SspI StuI SwaI TfiI
Tth111I XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 179 AAATTC [GA]^AATT[CT]
AflIII 935 ACACGT A^C[GA][CT]GT
ApaI 51 GGGCCC GGGCC^C
ApoI 179 AAATTC [GA]^AATT[CT]
AspHI 697 GAGCAC G[AT]GC[AT]^C
BalI 729 TGGCCA TGG^CCA
BanI 591 GGCACC G^G[CT][GA]CC
BbrPI 936 CACGTG CAC^GTG
BbsI 305 GTCTTC GAAGAC(2/6)
BcgI 908 CGATTGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 305 GTCTTC GAAGAC(2/6)
BsaAI 936 CACGTG [CT]AC^GT[GA]
BsiHKAI 697 GAGCAC G[AT]GC[AT]^C
BsmBI 105 GAGACG CGTCTC(1/5)
BsrBI 86 GAGCGG CCGCTC(-3/-3)
CpoI 291 CGGACCG CG^G[AT]CCG
DrdI 668 GACCAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 629 CTCTTC CTCTTC(1/4)
EcoT14I 759 CCAAGG C^C[AT][AT]GG
FseI 313 GGCCGGCC GGCCGG^CC
HgaI 152 GACGC GACGC(5/10)
Ksp632I 629 CTCTTC CTCTTC(1/4)
KspI 271 CCGCGG CCGC^GG
MluNI 729 TGGCCA TGG^CCA
MscI 729 TGGCCA TGG^CCA
PflMI 721 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 936 CACGTG CAC^GTG
PmlI 936 CACGTG CAC^GTG
PpuMI 949 GGGTCCC [GA]G^G[AT]CC[CT]
RsrII 291 CGGACCG CG^G[AT]CCG
SacII 271 CCGCGG CCGC^GG
SapI 628 GCTCTTC GCTCTTC(1/4)
SbfI 649 CCTGCAGG CCTGCA^GG
SgrAI 636 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 649 CCTGCAGG CCTGCA^GG
StyI 759 CCAAGG C^C[AT][AT]GG
TaqI 748 TCGA T^CGA
Tsp509I 180 AATT ^AATT
TthHB8I 748 TCGA T^CGA
Van91I 721 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 411 CCAACCTGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 383 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
508 CAGGTACTG
BanII 51 GGGCCC G[GA]GC[CT]^C
346 GAGCCC
BsaI 530 GAGACC GGTCTC(1/5)
424 GGTCTC
BseRI 343 GAGGAG GAGGAG(10/8)
683 GAGGAG
BsgI 692 GTGCAG GTGCAG(16/14)
818 GTGCAG
BsiEI 93 CGGCCG CG[GA][CT]^CG
312 CGGCCG
BspMI 652 GCAGGT ACCTGC(4/8)
688 GCAGGT
BssHII 29 GCGCGC G^CGCGC
111 GCGCGC
BstXI 355 CCACTACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
786 CCAGCCGCCTGG
DdeI 206 CTGAG C^T[ACGT]AG
877 CTTAG
DsaI 271 CCGCGG C^C[GA][CT]GG
780 CCACGG
EagI 93 CGGCCG C^GGCCG
312 CGGCCG
EclXI 93 CGGCCG C^GGCCG
312 CGGCCG
Eco52I 93 CGGCCG C^GGCCG
312 CGGCCG
HaeII 1 GGCGCT [GA]GCGC^[CT]
117 GGCGCT
MaeII 816 ACGT A^CGT
937 ACGT
MaeIII 480 GTGAC ^GT[ACGT]AC
797 GTCAC
MseI 965 TTAA T^TAA
986 TTAA
NlaIII 743 CATG CATG^
766 CATG
SexAI 414 ACCTGGT A^CC[AT]GGT
669 ACCAGGT
SmaI 61 CCCGGG CCC^GGG
298 CCCGGG
Tru9I 965 TTAA T^TAA
986 TTAA
Tsp45I 480 GTGAC ^GT[GC]AC
797 GTCAC
XmaCI 61 CCCGGG C^CCGGG
298 CCCGGG
XmaI 61 CCCGGG C^CCGGG
298 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIV PleI PstI PvuII
RsaI Sau96I ScrFI SfaNI SfcI
TseI TspRI