##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BbeI BbrPI BcgI
BciVI BfrI BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiHKAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HinfI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XcmI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 690 CGCG CG^CG
AcyI 22 GACGCC G[GA]^CG[CT]C
AfaI 254 GTAC GT^AC
BamHI 151 GGATCC G^GATCC
BanI 173 GGTGCC G^G[CT][GA]CC
BanII 265 GAGCCC G[GA]GC[CT]^C
BbvCI 335 CCTCAGC CCTCAGC(-5/-2)
BclI 244 TGATCA T^GATCA
BcnI 695 CCGGG CC^[GC]GG
BfaI 743 CTAG C^TAG
BglI 33 GCCGTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 966 AGATCT A^GATCT
BsaAI 453 TACGTG [CT]AC^GT[GA]
BsaHI 22 GACGCC G[GA]^CG[CT]C
BseRI 164 CTCCTC GAGGAG(10/8)
BsgI 314 CTGCAC GTGCAG(16/14)
BsiEI 799 CGACCG CG[GA][CT]^CG
BsmAI 279 GAGAC GTCTC(1/5)
Bsp1407I 253 TGTACA T^GTACA
BsrDI 413 CATTGC GCAATG(2/0)
BsrGI 253 TGTACA T^GTACA
BssSI 655 CTCGTG CACGAG(-5/-1)
BstUI 690 CGCG CG^CG
EarI 114 CTCTTC CTCTTC(1/4)
EcoNI 185 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 45 CCCGC CCCGC(4/6)
FbaI 244 TGATCA T^GATCA
Hin1I 22 GACGCC G[GA]^CG[CT]C
HincII 537 GTTAAC GT[CT]^[GA]AC
HindII 537 GTTAAC GT[CT]^[GA]AC
HpaI 537 GTTAAC GTT^AAC
Ksp632I 114 CTCTTC CTCTTC(1/4)
MaeI 743 CTAG C^TAG
MaeII 454 ACGT A^CGT
MvnI 690 CGCG CG^CG
NciI 695 CCGGG CC^[GC]GG
NcoI 982 CCATGG C^CATGG
NspI 936 GCATGT [GA]CATG^[CT]
PaeR7I 852 CTCGAG C^TCGAG
PpuMI 769 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 254 GTAC GT^AC
SapI 113 GCTCTTC GCTCTTC(1/4)
SfaNI 374 GATGC GCATC(5/9)
SfcI 392 CTACAG C^T[GA][CT]AG
SspBI 253 TGTACA T^GTACA
TaqI 853 TCGA T^CGA
Tsp509I 260 AATT ^AATT
TthHB8I 853 TCGA T^CGA
XhoI 852 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaI 852 CTCGAG C^[CT]CG[GA]G
888 CCCGAG
BalI 229 TGGCCA TGG^CCA
361 TGGCCA
BmyI 174 GTGCCC G[AGT]GC[ACT]^C
265 GAGCCC
BpmI 351 CTGGAG CTGGAG(16/14)
841 CTGGAG
BsoBI 852 CTCGAG C^[CT]CG[GA]G
888 CCCGAG
Bsp1286I 174 GTGCCC G[AGT]GC[ACT]^C
265 GAGCCC
BsrI 541 ACTGG ACTGG(1/-1)
76 CCAGT
BstEII 290 GGTCACC G^GT[ACGT]ACC
747 GGTCACC
BstF5I 248 CATCC GGATG(2/0)
927 GGATG
BstPI 290 GGTCACC G^GT[ACGT]ACC
747 GGTCACC
CfoI 340 GCGC GCG^C
893 GCGC
EcoO65I 290 GGTCACC G^GT[ACGT]ACC
747 GGTCACC
FokI 248 CATCC GGATG(9/13)
927 GGATG
HaeII 339 AGCGCC [GA]GCGC^[CT]
892 AGCGCC
HapII 558 CCGG C^CGG
695 CCGG
HhaI 340 GCGC GCG^C
893 GCGC
HinP1I 340 GCGC G^CGC
893 GCGC
HpaII 558 CCGG C^CGG
695 CCGG
MluNI 229 TGGCCA TGG^CCA
361 TGGCCA
MscI 229 TGGCCA TGG^CCA
361 TGGCCA
MslI 368 CATCACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
399 CACTACCATG
MspA1I 49 CAGCGG C[AC]G^C[GT]G
319 CCGCTG
MspI 558 CCGG C^CGG
695 CCGG
SmlI 514 CTTGAG C^T[CT][GA]AG
852 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AvaII BbsI
BbvI BpuAI BsaJI BsiYI BslI
BsmFI BssKI BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
HaeIII HgaI HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI StyI Tru9I
TseI Tsp45I TspRI XhoII