##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
KpnI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 1194 GTAGAC GT^[AC][GT]AC
AccII 662 CGCG CG^CG
AhdI 799 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 305 GATCC GGATC(4/5)
ApaI 865 GGGCCC GGGCC^C
AspEI 799 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1219 CCTAGG C^CTAGG
BalI 543 TGGCCA TGG^CCA
BbeI 108 GGCGCC GGCGC^C
BcnI 639 CCGGG CC^[GC]GG
BglI 1049 GCCCACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1219 CCTAGG C^CTAGG
BlpI 531 GCTTAGC GC^T[ACGT]AGC
BpmI 671 CTCCAG CTGGAG(16/14)
Bpu1102I 531 GCTTAGC GC^T[ACGT]AGC
BsgI 517 CTGCAC GTGCAG(16/14)
BspHI 585 TCATGA T^CATGA
BspLU11I 198 ACATGT A^CATGT
BstEII 115 GGTCACC G^GT[ACGT]ACC
BstPI 115 GGTCACC G^GT[ACGT]ACC
BstUI 662 CGCG CG^CG
BstXI 705 CCAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 304 AGATCC [GA]^GATC[CT]
CelII 531 GCTTAGC GC^T[ACGT]AGC
DraIII 899 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 977 GACCACTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 799 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 115 GGTCACC G^GT[ACGT]ACC
FauI 663 GCGGG CCCGC(4/6)
HapII 639 CCGG C^CGG
HincII 86 GTCAAC GT[CT]^[GA]AC
HindII 86 GTCAAC GT[CT]^[GA]AC
HindIII 260 AAGCTT A^AGCTT
HpaII 639 CCGG C^CGG
KasI 108 GGCGCC G^GCGCC
KspI 661 CCGCGG CCGC^GG
MaeIII 116 GTCAC ^GT[ACGT]AC
MflI 304 AGATCC [GA]^GATC[CT]
MluNI 543 TGGCCA TGG^CCA
MscI 543 TGGCCA TGG^CCA
MseI 132 TTAA T^TAA
MspA1I 661 CCGCGG C[AC]G^C[GT]G
MspI 639 CCGG C^CGG
MvnI 662 CGCG CG^CG
NarI 108 GGCGCC GG^CGCC
NciI 639 CCGGG CC^[GC]GG
NspI 198 ACATGT [GA]CATG^[CT]
PshAI 589 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 585 TCATGA T^CATGA
SacII 661 CCGCGG CCGC^GG
SexAI 900 ACCAGGT A^CC[AT]GGT
SfcI 1021 CTACAG C^T[GA][CT]AG
SfiI 1048 GGCCCACATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 108 GGCGCC GGC^GCC
TaqI 7 TCGA T^CGA
Tru9I 132 TTAA T^TAA
Tsp45I 116 GTCAC ^GT[GC]AC
TthHB8I 7 TCGA T^CGA
XbaI 808 TCTAGA T^CTAGA
XhoII 304 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 227 AAATTC [GA]^AATT[CT]
1013 GAATTT
AflIII 198 ACATGT A^C[GA][CT]GT
780 ACGTGT
ApoI 227 AAATTC [GA]^AATT[CT]
1013 GAATTT
BanI 108 GGCGCC G^G[CT][GA]CC
236 GGCACC
BbsI 619 GAAGAC GAAGAC(2/6)
365 GTCTTC
BbvI 125 GCTGC GCAGC(8/12)
701 GCTGC
BfaI 809 CTAG C^TAG
1220 CTAG
BpuAI 619 GAAGAC GAAGAC(2/6)
365 GTCTTC
BsaI 1117 GAGACC GGTCTC(1/5)
907 GGTCTC
BsmAI 1117 GAGAC GTCTC(1/5)
908 GTCTC
BspMI 800 ACCTGC ACCTGC(4/8)
817 ACCTGC
BsrDI 964 CATTGC GCAATG(2/0)
507 GCAATG
DpnII 305 GATC ^GATC
499 GATC
EaeI 543 TGGCCA [CT]^GGCC[GA]
658 TGGCCG
EarI 1110 GAAGAG CTCTTC(1/4)
1205 GAAGAG
EcoNI 541 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1201 CCTTGAAGAGG
Ksp632I 1110 GAAGAG CTCTTC(1/4)
1205 GAAGAG
MaeI 809 CTAG C^TAG
1220 CTAG
MaeII 345 ACGT A^CGT
780 ACGT
MboI 305 GATC ^GATC
499 GATC
NdeII 305 GATC ^GATC
499 GATC
Sau3AI 305 GATC ^GATC
499 GATC
SmlI 494 CTCAAG C^T[CT][GA]AG
872 CTCAAG
TfiI 212 GATTC G^A[AT]TC
1168 GAATC
TseI 125 GCTGC G^C[AT]GC
701 GCTGC
Tsp509I 228 AATT ^AATT
1014 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaII BanII BmyI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HgaI HhaI
Hin1I HinP1I HinfI HphI ItaI
MboII MnlI MslI MvaI MwoI
NcoI NlaIII NlaIV PleI RsaI
Sau96I ScrFI SfaNI StyI TspRI
XcmI