##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI BpmI Bpu1102I BpuAI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII HpaI KasI Ksp632I KspI
MfeI MluI MroI MslI MspA1I
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 74 GGTACC G^GTACC
AfaI 75 GTAC GT^AC
AocI 171 CCTGAGG CC^T[ACGT]AGG
Asp718I 74 GGTACC G^GTACC
AspHI 94 GAGCTC G[AT]GC[AT]^C
BbrPI 1129 CACGTG CAC^GTG
BfaI 1037 CTAG C^TAG
BglI 625 GCCTGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 670 AGATCT A^GATCT
BsaAI 1129 CACGTG [CT]AC^GT[GA]
BsaBI 280 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 366 ACCGGA [AT]^CCGG[AT]
BsgI 1086 CTGCAC GTGCAG(16/14)
BsiHKAI 94 GAGCTC G[AT]GC[AT]^C
BsmI 832 GCATTC GAATGC(1/-1)
BspLU11I 230 ACATGT A^CATGT
Bsu36I 171 CCTGAGG CC^T[ACGT]AGG
Eco81I 171 CCTGAGG CC^T[ACGT]AGG
FauI 981 CCCGC CCCGC(4/6)
HaeII 37 GGCGCT [GA]GCGC^[CT]
HincII 337 GTCAAC GT[CT]^[GA]AC
HindII 337 GTCAAC GT[CT]^[GA]AC
KpnI 74 GGTACC GGTAC^C
MaeI 1037 CTAG C^TAG
MaeII 1130 ACGT A^CGT
MaeIII 1186 GTTAC ^GT[ACGT]AC
MamI 280 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1122 CATATG CA^TATG
NspI 230 ACATGT [GA]CATG^[CT]
PmaCI 1129 CACGTG CAC^GTG
PmlI 1129 CACGTG CAC^GTG
PshAI 1127 GACACGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 181 CTGCAG CTGCA^G
RsaI 75 GTAC GT^AC
SacI 94 GAGCTC GAGCT^C
SfcI 181 CTGCAG C^T[GA][CT]AG
SmaI 1 CCCGGG CCC^GGG
StuI 1093 AGGCCT AGG^CCT
TaqI 162 TCGA T^CGA
Tsp509I 993 AATT ^AATT
TthHB8I 162 TCGA T^CGA
XcmI 697 CCAGTGGGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1 CCCGGG C^CCGGG
XmaI 1 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 9 CGCG CG^CG
11 CGCG
AlwI 189 GGATC GGATC(4/5)
530 GGATC
AlwNI 528 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
714 CAGTGGCTG
ApaI 176 GGGCCC GGGCC^C
1166 GGGCCC
AvaI 1 CCCGGG C^[CT]CG[GA]G
52 CCCGAG
BalI 565 TGGCCA TGG^CCA
1051 TGGCCA
BanI 74 GGTACC G^G[CT][GA]CC
867 GGTGCC
BseRI 91 GAGGAG GAGGAG(10/8)
103 GAGGAG
BsmAI 256 GAGAC GTCTC(1/5)
443 GAGAC
BsmFI 464 GGGAC GGGAC(10/14)
1213 GTCCC
BsoBI 1 CCCGGG C^[CT]CG[GA]G
52 CCCGAG
BsrI 697 CCAGT ACTGG(1/-1)
1216 CCAGT
BstUI 9 CGCG CG^CG
11 CGCG
BstXI 479 CCAGGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
871 CCATGCGATTGG
EcoT14I 357 CCAAGG C^C[AT][AT]GG
685 CCTTGG
MboII 205 GAAGA GAAGA(8/7)
917 GAAGA
MluNI 565 TGGCCA TGG^CCA
1051 TGGCCA
MscI 565 TGGCCA TGG^CCA
1051 TGGCCA
MvnI 9 CGCG CG^CG
11 CGCG
PleI 445 GACTC GAGTC(4/5)
1081 GAGTC
SmlI 421 CTCAAG C^T[CT][GA]AG
447 CTCAAG
StyI 357 CCAAGG C^C[AT][AT]GG
685 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AflIII AluI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MboI MflI MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI Sau3AI Sau96I
ScrFI SfaNI TfiI Tru9I TseI
TspRI XhoII