##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BciVI BclI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FauI FbaI FseI FspI HgaI
Hin1I HindIII KasI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 271 GGTACC G^GTACC
AflII 686 CTTAAG C^TTAAG
AflIII 775 ACATGT A^C[GA][CT]GT
AlwNI 437 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 271 GGTACC G^GTACC
AspHI 715 GAGCTC G[AT]GC[AT]^C
AvaII 105 GGACC G^G[AT]CC
BanI 271 GGTACC G^G[CT][GA]CC
BanII 715 GAGCTC G[GA]GC[CT]^C
BcgI 20 GCACCGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 57 CTAG C^TAG
BfrI 686 CTTAAG C^TTAAG
BlpI 51 GCTAAGC GC^T[ACGT]AGC
BmyI 715 GAGCTC G[AGT]GC[ACT]^C
BpmI 175 CTGGAG CTGGAG(16/14)
Bpu1102I 51 GCTAAGC GC^T[ACGT]AGC
BsaI 408 GAGACC GGTCTC(1/5)
BsaJI 260 CCGAGG C^C[ACGT][ACGT]GG
BsgI 18 CTGCAC GTGCAG(16/14)
BsiHKAI 715 GAGCTC G[AT]GC[AT]^C
BsiYI 174 CCTGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 174 CCTGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 70 CGTCTC CGTCTC(1/5)
Bsp1286I 715 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 775 ACATGT A^CATGT
BspMI 320 ACCTGC ACCTGC(4/8)
BsrBI 9 CCGCTC CCGCTC(-3/-3)
BssSI 46 CTCGTG CACGAG(-5/-1)
CelII 51 GCTAAGC GC^T[ACGT]AGC
DdeI 52 CTAAG C^T[ACGT]AG
DraI 809 TTTAAA TTT^AAA
EarI 718 CTCTTC CTCTTC(1/4)
EcoT22I 804 ATGCAT ATGCA^T
HaeIII 256 GGCC GG^CC
HincII 605 GTTAAC GT[CT]^[GA]AC
HindII 605 GTTAAC GT[CT]^[GA]AC
HpaI 605 GTTAAC GTT^AAC
KpnI 271 GGTACC GGTAC^C
Ksp632I 718 CTCTTC CTCTTC(1/4)
MaeI 57 CTAG C^TAG
MspA1I 248 CCGCTG C[AC]G^C[GT]G
NheI 56 GCTAGC G^CTAGC
NsiI 804 ATGCAT ATGCA^T
NspI 775 ACATGT [GA]CATG^[CT]
PleI 223 GACTC GAGTC(4/5)
PpuMI 104 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 700 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 715 GAGCTC GAGCT^C
SapI 717 GCTCTTC GCTCTTC(1/4)
SbfI 321 CCTGCAGG CCTGCA^GG
Sse8387I 321 CCTGCAGG CCTGCA^GG
TaqI 299 TCGA T^CGA
TfiI 510 GAATC G^A[AT]TC
Tsp45I 556 GTGAC ^GT[GC]AC
TspRI 291 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 299 TCGA T^CGA
XcmI 486 CCAGTTCTTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 24 CGCG CG^CG
159 CGCG
AfaI 272 GTAC GT^AC
360 GTAC
AluI 55 AGCT AG^CT
716 AGCT
AlwI 147 GATCC GGATC(4/5)
629 GGATC
AvaI 1 CCCGAG C^[CT]CG[GA]G
259 CCCGAG
BbvI 17 GCTGC GCAGC(8/12)
442 GCTGC
BcnI 102 CCGGG CC^[GC]GG
150 CCGGG
BglI 8 GCCGCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
257 GCCCCGAGGGC
BsmAI 408 GAGAC GTCTC(1/5)
71 GTCTC
BsmFI 104 GGGAC GGGAC(10/14)
698 GGGAC
BsoBI 1 CCCGAG C^[CT]CG[GA]G
259 CCCGAG
BspHI 89 TCATGA T^CATGA
308 TCATGA
BstNI 174 CCTGG CC^[AT]GG
680 CCAGG
BstUI 24 CGCG CG^CG
159 CGCG
BstYI 146 AGATCC [GA]^GATC[CT]
629 GGATCT
Cac8I 27 GCTCGC GC[ACGT]^[ACGT]GC
56 GCTAGC
Cfr13I 105 GGACC G^G[ACGT]CC
256 GGCCC
DraII 104 GGGACCT [GA]G^G[ACGT]CC[CT]
255 AGGCCCC
EcoO109I 104 GGGACCT [GA]G^G[ACGT]CC[CT]
255 AGGCCCC
EcoRII 174 CCTGG ^CC[AT]GG
680 CCAGG
HaeII 5 AGCGCC [GA]GCGC^[CT]
59 AGCGCC
HinfI 223 GACTC G^A[ACGT]TC
510 GAATC
MaeIII 212 GTAAC ^GT[ACGT]AC
556 GTGAC
MflI 146 AGATCC [GA]^GATC[CT]
629 GGATCT
MvaI 174 CCTGG CC^[AT]GG
680 CCAGG
MvnI 24 CGCG CG^CG
159 CGCG
NciI 102 CCGGG CC^[GC]GG
150 CCGGG
PstI 322 CTGCAG CTGCA^G
443 CTGCAG
RcaI 89 TCATGA T^CATGA
308 TCATGA
RsaI 272 GTAC GT^AC
360 GTAC
Sau96I 105 GGACC G^G[ACGT]CC
256 GGCCC
SfcI 322 CTGCAG C^T[GA][CT]AG
443 CTGCAG
SmlI 686 CTTAAG C^T[CT][GA]AG
712 CTTGAG
TseI 17 GCTGC G^C[AT]GC
442 GCTGC
Tsp509I 472 AATT ^AATT
620 AATT
XhoII 146 AGATCC [GA]^GATC[CT]
629 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI BsrI BssKI BstF5I CfoI
DpnII Fnu4HI FokI HapII HhaI
HinP1I HpaII HphI ItaI MboI
MboII MnlI MseI MspI MwoI
NdeII NlaIII NlaIV Sau3AI ScrFI
Tru9I