##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AvaII AvrII BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfaI
BglI BglII BlnI BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmBI BsmFI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoRI FbaI Hin1I
HincII HindII HindIII HpaI KasI
KspI MaeI MamI MfeI MflI
MluI MroI MspA1I MunI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TaqI TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
Acc65I 930 GGTACC G^GTACC
AcsI 924 AAATTT [GA]^AATT[CT]
AflII 886 CTTAAG C^TTAAG
ApoI 924 AAATTT [GA]^AATT[CT]
Asp718I 930 GGTACC G^GTACC
AspHI 305 GAGCTC G[AT]GC[AT]^C
AspI 229 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 778 CTCGGG C^[CT]CG[GA]G
AviII 690 TGCGCA TGC^GCA
BalI 585 TGGCCA TGG^CCA
BanI 930 GGTACC G^G[CT][GA]CC
BanII 305 GAGCTC G[GA]GC[CT]^C
BbrPI 677 CACGTG CAC^GTG
BfrI 886 CTTAAG C^TTAAG
BlpI 771 GCTAAGC GC^T[ACGT]AGC
BmyI 305 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 771 GCTAAGC GC^T[ACGT]AGC
BsaAI 677 CACGTG [CT]AC^GT[GA]
BseRI 407 GAGGAG GAGGAG(10/8)
BsgI 575 CTGCAC GTGCAG(16/14)
BsiHKAI 305 GAGCTC G[AT]GC[AT]^C
BsmI 517 GAATGC GAATGC(1/-1)
BsoBI 778 CTCGGG C^[CT]CG[GA]G
Bsp1286I 305 GAGCTC G[AGT]GC[ACT]^C
BspHI 403 TCATGA T^CATGA
BsrDI 521 GCAATG GCAATG(2/0)
BsrFI 98 GCCGGC [GA]^CCGG[CT]
BstAPI 827 GCAGTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 880 CATCC GGATG(2/0)
CelII 771 GCTAAGC GC^T[ACGT]AGC
Cfr10I 98 GCCGGC [GA]^CCGG[CT]
DpnII 295 GATC ^GATC
DraII 156 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 226 GACGACCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 799 CCGTGG C^C[GA][CT]GG
EarI 128 CTCTTC CTCTTC(1/4)
EcoO109I 156 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 476 GATATC GAT^ATC
EcoT22I 834 ATGCAT ATGCA^T
FokI 880 CATCC GGATG(9/13)
FseI 97 GGCCGGCC GGCCGG^CC
FspI 690 TGCGCA TGC^GCA
HgaI 233 GCGTC GACGC(5/10)
KpnI 930 GGTACC GGTAC^C
Ksp632I 128 CTCTTC CTCTTC(1/4)
MboI 295 GATC ^GATC
MluNI 585 TGGCCA TGG^CCA
MscI 585 TGGCCA TGG^CCA
MseI 887 TTAA T^TAA
NaeI 98 GCCGGC GCC^GGC
NdeII 295 GATC ^GATC
NgoMIV 98 GCCGGC G^CCGGC
NsiI 834 ATGCAT ATGCA^T
PflFI 229 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 677 CACGTG CAC^GTG
PmlI 677 CACGTG CAC^GTG
PstI 440 CTGCAG CTGCA^G
RcaI 403 TCATGA T^CATGA
SacI 305 GAGCTC GAGCT^C
Sau3AI 295 GATC ^GATC
SfcI 440 CTGCAG C^T[GA][CT]AG
SmlI 886 CTTAAG C^T[CT][GA]AG
SphI 832 GCATGC GCATG^C
Tru9I 887 TTAA T^TAA
Tth111I 229 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 657 CCATCAAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BbvI 183 GCAGC GCAGC(8/12)
439 GCTGC
BcnI 75 CCCGG CC^[GC]GG
94 CCCGG
BpmI 171 CTCCAG CTGGAG(16/14)
302 CTGGAG
BsiYI 88 CCCCACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
707 CCCGAATCTGG
BslI 88 CCCCACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
707 CCCGAATCTGG
BsmAI 626 GAGAC GTCTC(1/5)
753 GAGAC
BspMI 244 GCAGGT ACCTGC(4/8)
646 GCAGGT
BsrBI 168 CCGCTC CCGCTC(-3/-3)
508 GAGCGG
BstEII 671 GGTGACC G^GT[ACGT]ACC
817 GGTCACC
BstPI 671 GGTGACC G^GT[ACGT]ACC
817 GGTCACC
BstXI 448 CCAACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
793 CCATAGCCGTGG
Cfr13I 13 GGGCC G^G[ACGT]CC
157 GGCCC
EagI 96 CGGCCG C^GGCCG
100 CGGCCG
EclXI 96 CGGCCG C^GGCCG
100 CGGCCG
Eco52I 96 CGGCCG C^GGCCG
100 CGGCCG
EcoO65I 671 GGTGACC G^GT[ACGT]ACC
817 GGTCACC
EcoT14I 153 CCAAGG C^C[AT][AT]GG
822 CCAAGG
MslI 608 CATTTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
794 CATAGCCGTG
NciI 75 CCCGG CC^[GC]GG
94 CCCGG
PleI 269 GACTC GAGTC(4/5)
34 GAGTC
Sau96I 13 GGGCC G^G[ACGT]CC
157 GGCCC
StyI 153 CCAAGG C^C[AT][AT]GG
822 CCAAGG
TseI 183 GCAGC G^C[AT]GC
439 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AfaI AluI BsaJI
BsiEI BsrI BssKI BstNI BstUI
Cac8I CfoI DdeI EaeI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboII MnlI
MspI MvaI MvnI MwoI NlaIII
NlaIV NspI RsaI ScrFI TfiI
Tsp45I Tsp509I TspRI