##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AviII AvrII BamHI
BbeI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 710 GACGCC G[GA]^CG[CT]C
AfaI 722 GTAC GT^AC
AhdI 405 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 69 GGGCCC GGGCC^C
AspEI 405 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 769 CCCGAG C^[CT]CG[GA]G
BalI 625 TGGCCA TGG^CCA
BanII 69 GGGCCC G[GA]GC[CT]^C
BbrPI 741 CACGTG CAC^GTG
BbvCI 5 GCTGAGG CCTCAGC(-5/-2)
BciVI 837 GGATAC GTATCC(6/5)
BfaI 402 CTAG C^TAG
BglI 759 GCCTTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 741 CACGTG [CT]AC^GT[GA]
BsaHI 710 GACGCC G[GA]^CG[CT]C
BsoBI 769 CCCGAG C^[CT]CG[GA]G
BssSI 122 CTCGTG CACGAG(-5/-1)
Eam1105I 405 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 139 CCATGG C^C[AT][AT]GG
HgaI 710 GACGC GACGC(5/10)
Hin1I 710 GACGCC G[GA]^CG[CT]C
HincII 486 GTCAAC GT[CT]^[GA]AC
HindII 486 GTCAAC GT[CT]^[GA]AC
MaeI 402 CTAG C^TAG
MluNI 625 TGGCCA TGG^CCA
MscI 625 TGGCCA TGG^CCA
MslI 809 CACCCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 392 GCCGGC GCC^GGC
NcoI 139 CCATGG C^CATGG
NgoMIV 392 GCCGGC G^CCGGC
NlaIII 140 CATG CATG^
PflMI 587 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 741 CACGTG CAC^GTG
PmlI 741 CACGTG CAC^GTG
PvuII 450 CAGCTG CAG^CTG
RsaI 722 GTAC GT^AC
SapI 881 GCTCTTC GCTCTTC(1/4)
SexAI 895 ACCTGGT A^CC[AT]GGT
SfaNI 303 GATGC GCATC(5/9)
SfiI 758 GGCCTTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 876 CTCAAG C^T[CT][GA]AG
StuI 757 AGGCCT AGG^CCT
StyI 139 CCATGG C^C[AT][AT]GG
TaqI 862 TCGA T^CGA
TfiI 290 GAATC G^A[AT]TC
TthHB8I 862 TCGA T^CGA
Van91I 587 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 244 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
1002 CAGCTTCTG
BanI 576 GGTGCC G^G[CT][GA]CC
899 GGTGCC
BbsI 162 GAAGAC GAAGAC(2/6)
213 GAAGAC
BcnI 219 CCCGG CC^[GC]GG
931 CCGGG
BmyI 69 GGGCCC G[AGT]GC[ACT]^C
900 GTGCCC
BpuAI 162 GAAGAC GAAGAC(2/6)
213 GAAGAC
BsaI 549 GAGACC GGTCTC(1/5)
16 GGTCTC
BseRI 115 GAGGAG GAGGAG(10/8)
225 GAGGAG
BsgI 272 CTGCAC GTGCAG(16/14)
1060 GTGCAG
Bsp1286I 69 GGGCCC G[AGT]GC[ACT]^C
900 GTGCCC
BsrFI 361 ACCGGC [GA]^CCGG[CT]
392 GCCGGC
BsrI 90 ACTGG ACTGG(1/-1)
307 CCAGT
Cfr10I 361 ACCGGC [GA]^CCGG[CT]
392 GCCGGC
DpnII 1 GATC ^GATC
860 GATC
DrdI 246 GACACTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
477 GACAACCTCGTC
DsaI 139 CCATGG C^C[GA][CT]GG
826 CCGTGG
EaeI 394 CGGCCA [CT]^GGCC[GA]
625 TGGCCA
EarI 882 CTCTTC CTCTTC(1/4)
48 GAAGAG
HaeII 612 AGCGCC [GA]GCGC^[CT]
774 GGCGCT
Ksp632I 882 CTCTTC CTCTTC(1/4)
48 GAAGAG
MaeII 676 ACGT A^CGT
742 ACGT
MboI 1 GATC ^GATC
860 GATC
MspA1I 450 CAGCTG C[AC]G^C[GT]G
904 CCGCTG
NciI 219 CCCGG CC^[GC]GG
931 CCGGG
NdeII 1 GATC ^GATC
860 GATC
PpuMI 205 GGGACCC [GA]G^G[AT]CC[CT]
1026 GGGACCC
PstI 157 CTGCAG CTGCA^G
232 CTGCAG
Sau3AI 1 GATC ^GATC
860 GATC
SfcI 157 CTGCAG C^T[GA][CT]AG
232 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BpmI BsaJI BsaWI BsiYI BslI
BsmAI BsmFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MspI MvaI MvnI
MwoI NlaIV PleI Sau96I ScrFI
TseI Tsp45I TspRI