##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanII BbrPI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I CpoI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FauI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MflI MluI MroI MslI
MunI MvnI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AlwNI 1106 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1224 ATTAAT AT^TAAT
AsnI 1224 ATTAAT AT^TAAT
AspHI 1203 GTGCTC G[AT]GC[AT]^C
AvaI 413 CCCGAG C^[CT]CG[GA]G
AvaII 694 GGACC G^G[AT]CC
BalI 143 TGGCCA TGG^CCA
BanI 649 GGCGCC G^G[CT][GA]CC
BbeI 649 GGCGCC GGCGC^C
BbsI 988 GAAGAC GAAGAC(2/6)
BclI 368 TGATCA T^GATCA
BglI 515 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 988 GAAGAC GAAGAC(2/6)
BsiHKAI 1203 GTGCTC G[AT]GC[AT]^C
BsmBI 71 GAGACG CGTCTC(1/5)
BsmFI 939 GGGAC GGGAC(10/14)
BsoBI 413 CCCGAG C^[CT]CG[GA]G
Bsp1407I 744 TGTACA T^GTACA
BspDI 172 ATCGAT AT^CGAT
BsrBI 114 GAGCGG CCGCTC(-3/-3)
BsrGI 744 TGTACA T^GTACA
BstF5I 354 GGATG GGATG(2/0)
CfoI 650 GCGC GCG^C
ClaI 172 ATCGAT AT^CGAT
DraII 693 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 685 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 693 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1044 CCATGG C^C[AT][AT]GG
EcoT22I 298 ATGCAT ATGCA^T
FbaI 368 TGATCA T^GATCA
FokI 354 GGATG GGATG(9/13)
HaeII 649 GGCGCC [GA]GCGC^[CT]
HhaI 650 GCGC GCG^C
HinP1I 650 GCGC G^CGC
HinfI 642 GAATC G^A[ACGT]TC
KasI 649 GGCGCC G^GCGCC
MaeII 844 ACGT A^CGT
MluNI 143 TGGCCA TGG^CCA
MscI 143 TGGCCA TGG^CCA
NarI 649 GGCGCC GG^CGCC
NcoI 1044 CCATGG C^CATGG
NdeI 437 CATATG CA^TATG
NsiI 298 ATGCAT ATGCA^T
PpuMI 693 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1224 ATTAAT AT^TAAT
SfoI 649 GGCGCC GGC^GCC
SmlI 811 CTTGAG C^T[CT][GA]AG
SphI 300 GCATGC GCATG^C
SspBI 744 TGTACA T^GTACA
StyI 1044 CCATGG C^C[AT][AT]GG
TaqI 173 TCGA T^CGA
TfiI 642 GAATC G^A[AT]TC
TthHB8I 173 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 105 GGCGTC G[GA]^CG[CT]C
649 GGCGCC
BcnI 32 CCCGG CC^[GC]GG
110 CCGGG
BmyI 402 GGGCAC G[AGT]GC[ACT]^C
1203 GTGCTC
BsaHI 105 GGCGTC G[GA]^CG[CT]C
649 GGCGCC
Bsp1286I 402 GGGCAC G[AGT]GC[ACT]^C
1203 GTGCTC
DraI 1164 TTTAAA TTT^AAA
1247 TTTAAA
DsaI 5 CCGTGG C^C[GA][CT]GG
1044 CCATGG
EaeI 143 TGGCCA [CT]^GGCC[GA]
480 TGGCCG
HapII 33 CCGG C^CGG
110 CCGG
HgaI 57 GCGTC GACGC(5/10)
106 GCGTC
Hin1I 105 GGCGTC G[GA]^CG[CT]C
649 GGCGCC
HpaII 33 CCGG C^CGG
110 CCGG
MaeIII 1157 GTAAC ^GT[ACGT]AC
1174 GTTAC
MspA1I 934 CAGCTG C[AC]G^C[GT]G
1017 CAGCTG
MspI 33 CCGG C^CGG
110 CCGG
NciI 32 CCCGG CC^[GC]GG
110 CCGGG
NlaIV 585 GGAACC GG[ACGT]^[ACGT]CC
649 GGCGCC
NspI 152 ACATGC [GA]CATG^[CT]
300 GCATGC
PvuII 934 CAGCTG CAG^CTG
1017 CAGCTG
Tsp509I 806 AATT ^AATT
1089 AATT
XcmI 365 CCATGATCATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
516 CCACCCTGGCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HphI ItaI MaeI MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SspI Tru9I TseI
TspRI