##################################
# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BsrFI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflII 810 CTTAAG C^TTAAG
AlwI 1379 GATCC GGATC(4/5)
AocI 51 CCTGAGG CC^T[ACGT]AGG
ApaI 125 GGGCCC GGGCC^C
AspHI 39 GAGCAC G[AT]GC[AT]^C
BbvI 774 GCTGC GCAGC(8/12)
BcnI 177 CCCGG CC^[GC]GG
BfrI 810 CTTAAG C^TTAAG
BglI 22 GCCTGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 960 CTGGAG CTGGAG(16/14)
BseRI 54 GAGGAG GAGGAG(10/8)
BsiHKAI 39 GAGCAC G[AT]GC[AT]^C
BsmFI 214 GGGAC GGGAC(10/14)
Bsp1407I 1238 TGTACA T^GTACA
BspHI 913 TCATGA T^CATGA
BsrBI 158 GAGCGG CCGCTC(-3/-3)
BsrGI 1238 TGTACA T^GTACA
BstBI 507 TTCGAA TT^CGAA
BstYI 1378 AGATCC [GA]^GATC[CT]
Bsu36I 51 CCTGAGG CC^T[ACGT]AGG
DpnII 1379 GATC ^GATC
DsaI 210 CCGTGG C^C[GA][CT]GG
EaeI 235 TGGCCG [CT]^GGCC[GA]
Eco81I 51 CCTGAGG CC^T[ACGT]AGG
EcoRI 504 GAATTC G^AATTC
HindIII 791 AAGCTT A^AGCTT
HinfI 252 GACTC G^A[ACGT]TC
MboI 1379 GATC ^GATC
MflI 1378 AGATCC [GA]^GATC[CT]
MslI 72 CACCGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1016 CAGCTG C[AC]G^C[GT]G
NciI 177 CCCGG CC^[GC]GG
NdeII 1379 GATC ^GATC
NspV 507 TTCGAA TT^CGAA
PleI 252 GACTC GAGTC(4/5)
PvuII 1016 CAGCTG CAG^CTG
RcaI 913 TCATGA T^CATGA
Sau3AI 1379 GATC ^GATC
SfiI 21 GGCCTGAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 507 TTCGAA TT^CGAA
SmlI 810 CTTAAG C^T[CT][GA]AG
SspBI 1238 TGTACA T^GTACA
SspI 524 AATATT AAT^ATT
TaqI 508 TCGA T^CGA
TseI 774 GCTGC G^C[AT]GC
TthHB8I 508 TCGA T^CGA
XcmI 595 CCAATGCAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1378 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanII 81 GAGCCC G[GA]GC[CT]^C
125 GGGCCC
BfaI 108 CTAG C^TAG
1265 CTAG
BsaI 146 GGTCTC GGTCTC(1/5)
180 GGTCTC
BsmAI 147 GTCTC GTCTC(1/5)
181 GTCTC
BspMI 12 ACCTGC ACCTGC(4/8)
1316 ACCTGC
BsrDI 600 GCAATG GCAATG(2/0)
666 GCAATG
BstNI 122 CCTGG CC^[AT]GG
190 CCTGG
EcoRII 122 CCTGG ^CC[AT]GG
190 CCTGG
HapII 178 CCGG C^CGG
256 CCGG
HincII 59 GTTGAC GT[CT]^[GA]AC
1021 GTTGAC
HindII 59 GTTGAC GT[CT]^[GA]AC
1021 GTTGAC
HpaII 178 CCGG C^CGG
256 CCGG
HphI 501 GGTGA GGTGA(8/7)
71 TCACC
MaeI 108 CTAG C^TAG
1265 CTAG
MspI 178 CCGG C^CGG
256 CCGG
MvaI 122 CCTGG CC^[AT]GG
190 CCTGG
NdeI 403 CATATG CA^TATG
475 CATATG
NlaIV 125 GGGCCC GG[ACGT]^[ACGT]CC
711 GGAGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstUI
Cac8I CfoI Cfr13I DdeI DraI
FauI Fnu4HI FokI HaeIII HhaI
HinP1I ItaI MaeIII MboII MnlI
MseI MvnI MwoI NlaIII RsaI
Sau96I ScrFI SfaNI Tru9I Tsp509I
TspRI