##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI Aor51HI ApoI AseI AsnI
Asp718I AviII BamHI BbrPI BcgI
BciVI BclI BfrI BsaAI BsiWI
BsmBI Bsp1407I BspDI BspHI BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MfeI MluI MunI
NheI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1282 GTAGAC GT^[AC][GT]AC
AflIII 841 ACATGT A^C[GA][CT]GT
AhdI 993 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2546 CCTGAGG CC^T[ACGT]AGG
AscI 118 GGCGCGCC GG^CGCGCC
AspEI 993 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1072 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 573 CCTAGG C^CTAGG
BbeI 2786 GGCGCC GGCGC^C
BglII 890 AGATCT A^GATCT
BlnI 573 CCTAGG C^CTAGG
BsaBI 816 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 808 GAGACC GGTCTC(1/5)
BspLU11I 841 ACATGT A^CATGT
BspMI 1278 GCAGGT ACCTGC(4/8)
BssHII 119 GCGCGC G^CGCGC
BssSI 1929 CTCGTG CACGAG(-5/-1)
Bsu36I 2546 CCTGAGG CC^T[ACGT]AGG
DrdI 287 GACTGTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 993 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2546 CCTGAGG CC^T[ACGT]AGG
KasI 2786 GGCGCC G^GCGCC
KspI 17 CCGCGG CCGC^GG
MamI 816 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2786 GGCGCC GG^CGCC
NdeI 377 CATATG CA^TATG
NotI 13 GCGGCCGC GC^GGCCGC
NspI 841 ACATGT [GA]CATG^[CT]
PflFI 1072 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 17 CCGCGG CCGC^GG
SexAI 1019 ACCTGGT A^CC[AT]GGT
SfoI 2786 GGCGCC GGC^GCC
TaqI 145 TCGA T^CGA
TfiI 2805 GATTC G^A[AT]TC
Tsp509I 2390 AATT ^AATT
Tth111I 1072 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 145 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 443 TCCGGA T^CCGGA
665 TCCGGA
AfaI 621 GTAC GT^AC
1659 GTAC
Alw44I 2407 GTGCAC G^TGCAC
2799 GTGCAC
ApaLI 2407 GTGCAC G^TGCAC
2799 GTGCAC
BalI 1206 TGGCCA TGG^CCA
2327 TGGCCA
BfaI 338 CTAG C^TAG
574 CTAG
BglI 1359 GCCTGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2601 GCCTCGGCGGC
BsaWI 443 TCCGGA [AT]^CCGG[AT]
665 TCCGGA
BseAI 443 TCCGGA T^CCGGA
665 TCCGGA
BsiEI 14 CGGCCG CG[GA][CT]^CG
1488 CGGCCG
BsmI 975 GAATGC GAATGC(1/-1)
2126 GCATTC
BspEI 443 TCCGGA T^CCGGA
665 TCCGGA
BsrBI 1528 GAGCGG CCGCTC(-3/-3)
1935 GAGCGG
EagI 14 CGGCCG C^GGCCG
1488 CGGCCG
EclXI 14 CGGCCG C^GGCCG
1488 CGGCCG
Eco52I 14 CGGCCG C^GGCCG
1488 CGGCCG
MaeI 338 CTAG C^TAG
574 CTAG
MluNI 1206 TGGCCA TGG^CCA
2327 TGGCCA
MroI 443 TCCGGA T^CCGGA
665 TCCGGA
MscI 1206 TGGCCA TGG^CCA
2327 TGGCCA
MseI 383 TTAA T^TAA
2824 TTAA
MslI 501 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1596 CACAGACGTG
NcoI 503 CCATGG C^CATGG
1251 CCATGG
RsaI 621 GTAC GT^AC
1659 GTAC
SapI 1545 GCTCTTC GCTCTTC(1/4)
2259 GCTCTTC
Tru9I 383 TTAA T^TAA
2824 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TseI Tsp45I
TspRI Van91I XhoII XmaCI XmaI
XmnI