##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AgeI AlwNI Aor51HI AscI AseI
AsnI AvaI AviII BamHI BclI
BglII BsiWI BsmI BsoBI Bsp1407I
BspDI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Cfr10I ClaI
CpoI Eco47III EcoNI EcoO65I EcoRV
FbaI FseI FspI HincII HindII
HpaI MfeI MflI MluI MunI
NaeI NdeI NgoMIV NheI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI PvuI RcaI RsrII
SalI SbfI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Van91I XbaI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1585 GACGTC GACGT^C
Acc65I 2399 GGTACC G^GTACC
AccI 1995 GTAGAC GT^[AC][GT]AC
AccIII 198 TCCGGA T^CCGGA
AclI 2908 AACGTT AA^CGTT
AflII 2152 CTTAAG C^TTAAG
AhdI 189 GACGCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 544 GTGCAC G^TGCAC
AlwI 2417 GATCC GGATC(4/5)
ApaLI 544 GTGCAC G^TGCAC
Asp718I 2399 GGTACC G^GTACC
AspEI 189 GACGCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3440 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 793 TGGCCA TGG^CCA
BbeI 139 GGCGCC GGCGC^C
BbrPI 334 CACGTG CAC^GTG
BcgI 511 CGACGTTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2152 CTTAAG C^TTAAG
BlpI 2347 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2347 GCTGAGC GC^T[ACGT]AGC
BsaAI 334 CACGTG [CT]AC^GT[GA]
BsaBI 1400 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 198 TCCGGA T^CCGGA
BsiEI 172 CGGCCG CG[GA][CT]^CG
BsmBI 1587 CGTCTC CGTCTC(1/5)
BspEI 198 TCCGGA T^CCGGA
BspLU11I 758 ACATGT A^CATGT
CelII 2347 GCTGAGC GC^T[ACGT]AGC
DraIII 2981 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 172 CGGCCG C^GGCCG
Eam1105I 189 GACGCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 172 CGGCCG C^GGCCG
Eco52I 172 CGGCCG C^GGCCG
EcoRI 2369 GAATTC G^AATTC
EcoT22I 493 ATGCAT ATGCA^T
KasI 139 GGCGCC G^GCGCC
KpnI 2399 GGTACC GGTAC^C
KspI 175 CCGCGG CCGC^GG
MamI 1400 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 793 TGGCCA TGG^CCA
MroI 198 TCCGGA T^CCGGA
MscI 793 TGGCCA TGG^CCA
NarI 139 GGCGCC GG^CGCC
NotI 171 GCGGCCGC GC^GGCCGC
NruI 396 TCGCGA TCG^CGA
NsiI 493 ATGCAT ATGCA^T
PflFI 3440 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 334 CACGTG CAC^GTG
PmlI 334 CACGTG CAC^GTG
Psp1406I 2908 AACGTT AA^CGTT
SacII 175 CCGCGG CCGC^GG
SapI 1918 GCTCTTC GCTCTTC(1/4)
ScaI 1761 AGTACT AGT^ACT
SfiI 1139 GGCCGGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 139 GGCGCC GGC^GCC
Tth111I 3440 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 176 CGCG CG^CG
397 CGCG
AcyI 139 GGCGCC G[GA]^CG[CT]C
1585 GACGTC
AocI 82 CCTCAGG CC^T[ACGT]AGG
1352 CCTCAGG
Asp700I 1973 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2747 GAAGCAGTTC
AvrII 1638 CCTAGG C^CTAGG
3423 CCTAGG
BbsI 754 GAAGAC GAAGAC(2/6)
424 GTCTTC
BbvCI 3228 GCTGAGG CCTCAGC(-5/-2)
3361 GCTGAGG
BlnI 1638 CCTAGG C^CTAGG
3423 CCTAGG
BpuAI 754 GAAGAC GAAGAC(2/6)
424 GTCTTC
BsaHI 139 GGCGCC G[GA]^CG[CT]C
1585 GACGTC
BsgI 3412 CTGCAC GTGCAG(16/14)
2364 GTGCAG
BsrDI 663 GCAATG GCAATG(2/0)
1278 GCAATG
BstAPI 111 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
868 GCAGACCGTGC
BstUI 176 CGCG CG^CG
397 CGCG
Bsu36I 82 CCTCAGG CC^T[ACGT]AGG
1352 CCTCAGG
DraI 2001 TTTAAA TTT^AAA
2282 TTTAAA
DrdI 52 GACGTGAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1945 GACAAACGTGTC
Eco81I 82 CCTCAGG CC^T[ACGT]AGG
1352 CCTCAGG
HgaI 189 GACGC GACGC(5/10)
670 GACGC
Hin1I 139 GGCGCC G[GA]^CG[CT]C
1585 GACGTC
HindIII 2556 AAGCTT A^AGCTT
3157 AAGCTT
MslI 1697 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3146 CACTACTGTG
MvnI 176 CGCG CG^CG
397 CGCG
NcoI 1101 CCATGG C^CATGG
1380 CCATGG
SmlI 1432 CTTGAG C^T[CT][GA]AG
2152 CTTAAG
SspI 2005 AATATT AAT^ATT
2277 AATATT
StuI 1641 AGGCCT AGG^CCT
2711 AGGCCT
Tsp45I 945 GTGAC ^GT[GC]AC
1477 GTCAC
XcmI 276 CCAGGGGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2963 CCACTTTCTCACTGG
XmnI 1973 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2747 GAAGCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApaI ApoI AspHI AvaII BanI
BanII BbvI BciVI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I