##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
Alw44I ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BbrPI
BclI BfrI BlnI BsaAI BsaBI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SbfI ScaI SfiI SfuI
SnaBI SpeI Sse8387I SspBI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 670 GACGTC GACGT^C
AccI 30 GTCGAC GT^[AC][GT]AC
AcsI 736 AAATTC [GA]^AATT[CT]
AgeI 3040 ACCGGT A^CCGGT
AhdI 40 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1370 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 167 AGCGCT AGC^GCT
ApoI 736 AAATTC [GA]^AATT[CT]
Asp700I 2019 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 40 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1099 GGATCC G^GATCC
BbvCI 1621 GCTGAGG CCTCAGC(-5/-2)
BciVI 309 GGATAC GTATCC(6/5)
BglI 1698 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2462 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2462 GCTCAGC GC^T[ACGT]AGC
BsaWI 3040 ACCGGT [AT]^CCGG[AT]
BsrDI 1811 GCAATG GCAATG(2/0)
BsrFI 3040 ACCGGT [GA]^CCGG[CT]
BstAPI 201 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2462 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3040 ACCGGT [GA]^CCGG[CT]
DraI 2874 TTTAAA TTT^AAA
DraIII 1637 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3085 GACACTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 40 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 167 AGCGCT AGC^GCT
MslI 174 CACCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 81 CCATGG C^CATGG
NruI 2237 TCGCGA TCG^CGA
PflMI 3184 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3040 ACCGGT A^CCGGT
SalI 30 GTCGAC G^TCGAC
SapI 636 GCTCTTC GCTCTTC(1/4)
SexAI 122 ACCTGGT A^CC[AT]GGT
SgrAI 3039 CACCGGTG C[GA]^CCGG[CT]G
SspI 2955 AATATT AAT^ATT
SwaI 2873 ATTTAAAT ATTT^AAAT
Tsp509I 737 AATT ^AATT
Van91I 3184 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 525 TCTAGA T^CTAGA
XcmI 2701 CCAAGCCAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2019 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 675 CCTGAGG CC^T[ACGT]AGG
1417 CCTGAGG
BbeI 1661 GGCGCC GGCGC^C
1753 GGCGCC
BbsI 387 GAAGAC GAAGAC(2/6)
727 GAAGAC
BcgI 1702 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1595 GCAGCATGATCG
BpuAI 387 GAAGAC GAAGAC(2/6)
727 GAAGAC
BsaI 2736 GAGACC GGTCTC(1/5)
1311 GGTCTC
BsmBI 892 GAGACG CGTCTC(1/5)
1606 GAGACG
BsrBI 1049 CCGCTC CCGCTC(-3/-3)
1866 GAGCGG
Bsu36I 675 CCTGAGG CC^T[ACGT]AGG
1417 CCTGAGG
Eco81I 675 CCTGAGG CC^T[ACGT]AGG
1417 CCTGAGG
HgaI 1668 GACGC GACGC(5/10)
2309 GCGTC
KasI 1661 GGCGCC G^GCGCC
1753 GGCGCC
KspI 1994 CCGCGG CCGC^GG
2740 CCGCGG
NarI 1661 GGCGCC GG^CGCC
1753 GGCGCC
SacI 1350 GAGCTC GAGCT^C
2460 GAGCTC
SacII 1994 CCGCGG CCGC^GG
2740 CCGCGG
SfoI 1661 GGCGCC GGC^GCC
1753 GGCGCC
SmaI 1271 CCCGGG CCC^GGG
3033 CCCGGG
XmaCI 1271 CCCGGG C^CCGGG
3033 CCCGGG
XmaI 1271 CCCGGG C^CCGGG
3033 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglII BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I XhoII