##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccIII AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbvCI
BglI BlnI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I FseI
FspI KasI KpnI KspI MluI
MroI NarI NotI NruI NspV
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 3549 GACGTC GACGT^C
AccII 5443 CGCG CG^CG
AcyI 3549 GACGTC G[GA]^CG[CT]C
AgeI 1476 ACCGGT A^CCGGT
ApaI 3268 GGGCCC GGGCC^C
AspI 5120 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 4771 CGATGCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 6735 GGATAC GTATCC(6/5)
BglII 4236 AGATCT A^GATCT
BlpI 6232 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 6232 GCTGAGC GC^T[ACGT]AGC
BsaBI 5618 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3549 GACGTC G[GA]^CG[CT]C
BsrBI 5510 GAGCGG CCGCTC(-3/-3)
BstUI 5443 CGCG CG^CG
CelII 6232 GCTGAGC GC^T[ACGT]AGC
DrdI 3845 GACTTCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1040 CCATGG C^C[GA][CT]GG
EcoRV 1858 GATATC GAT^ATC
HaeII 5467 GGCGCT [GA]GCGC^[CT]
Hin1I 3549 GACGTC G[GA]^CG[CT]C
MamI 5618 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 8629 CAATTG C^AATTG
MunI 8629 CAATTG C^AATTG
MvnI 5443 CGCG CG^CG
NaeI 2124 GCCGGC GCC^GGC
NcoI 1040 CCATGG C^CATGG
NdeI 2603 CATATG CA^TATG
NgoMIV 2124 GCCGGC G^CCGGC
PacI 7556 TTAATTAA TTAAT^TAA
PaeR7I 5814 CTCGAG C^TCGAG
PflFI 5120 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1476 ACCGGT A^CCGGT
SexAI 3313 ACCTGGT A^CC[AT]GGT
SmaI 5259 CCCGGG CCC^GGG
SpeI 7074 ACTAGT A^CTAGT
StuI 1768 AGGCCT AGG^CCT
Tth111I 5120 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5814 CTCGAG C^TCGAG
XmaCI 5259 CCCGGG C^CCGGG
XmaI 5259 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AclI 3027 AACGTT AA^CGTT
8226 AACGTT
AocI 528 CCTAAGG CC^T[ACGT]AGG
3675 CCTAAGG
BalI 3731 TGGCCA TGG^CCA
5583 TGGCCA
BanI 3117 GGTGCC G^G[CT][GA]CC
3201 GGCACC
BsaI 6710 GAGACC GGTCTC(1/5)
3757 GGTCTC
BsaWI 1476 ACCGGT [AT]^CCGG[AT]
3413 TCCGGT
BspHI 5713 TCATGA T^CATGA
8220 TCATGA
BsrFI 1476 ACCGGT [GA]^CCGG[CT]
2124 GCCGGC
BssSI 2808 CACGAG CACGAG(-5/-1)
5063 CTCGTG
BstAPI 991 GCAGTTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4509 GCATACTCTGC
BstEII 3309 GGTAACC G^GT[ACGT]ACC
8634 GGTTACC
BstPI 3309 GGTAACC G^GT[ACGT]ACC
8634 GGTTACC
BstYI 427 GGATCT [GA]^GATC[CT]
4236 AGATCT
Bsu36I 528 CCTAAGG CC^T[ACGT]AGG
3675 CCTAAGG
CfoI 5468 GCGC GCG^C
5486 GCGC
Cfr10I 1476 ACCGGT [GA]^CCGG[CT]
2124 GCCGGC
EaeI 3731 TGGCCA [CT]^GGCC[GA]
5583 TGGCCA
Eco81I 528 CCTAAGG CC^T[ACGT]AGG
3675 CCTAAGG
EcoO65I 3309 GGTAACC G^GT[ACGT]ACC
8634 GGTTACC
FauI 5441 CCCGC CCCGC(4/6)
128 GCGGG
HgaI 4561 GCGTC GACGC(5/10)
5444 GCGTC
HhaI 5468 GCGC GCG^C
5486 GCGC
HinP1I 5468 GCGC G^CGC
5486 GCGC
MflI 427 GGATCT [GA]^GATC[CT]
4236 AGATCT
MluNI 3731 TGGCCA TGG^CCA
5583 TGGCCA
MscI 3731 TGGCCA TGG^CCA
5583 TGGCCA
NheI 5075 GCTAGC G^CTAGC
8094 GCTAGC
PpuMI 1940 AGGACCC [GA]G^G[AT]CC[CT]
4919 AGGACCC
Psp1406I 3027 AACGTT AA^CGTT
8226 AACGTT
RcaI 5713 TCATGA T^CATGA
8220 TCATGA
SapI 1245 GAAGAGC GCTCTTC(1/4)
6110 GAAGAGC
SnaBI 3968 TACGTA TAC^GTA
4448 TACGTA
SphI 3997 GCATGC GCATG^C
6064 GCATGC
TaqI 5803 TCGA T^CGA
5815 TCGA
TthHB8I 5803 TCGA T^CGA
5815 TCGA
XcmI 5571 CCAACAAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7374 CCATCACAAATGTGG
XhoII 427 GGATCT [GA]^GATC[CT]
4236 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BanII BbsI BbvI BclI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DraI DraII DraIII
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HapII HincII HindII HindIII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PshBI PstI PvuII RsaI
SacI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XbaI XmnI