##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BclI BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BstBI ClaI
CpoI DraI DraIII DrdI Eam1105I
EcoRV EcoT22I FbaI FseI FspI
HpaI KpnI MamI MfeI MluI
MroI MunI NdeI NheI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 2482 GAATTC [GA]^AATT[CT]
AflII 1344 CTTAAG C^TTAAG
Aor51HI 172 AGCGCT AGC^GCT
ApaI 976 GGGCCC GGGCC^C
ApoI 2482 GAATTC [GA]^AATT[CT]
AseI 2613 ATTAAT AT^TAAT
AsnI 2613 ATTAAT AT^TAAT
BfrI 1344 CTTAAG C^TTAAG
BglII 1144 AGATCT A^GATCT
BlpI 513 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 513 GCTGAGC GC^T[ACGT]AGC
BsmI 1991 GAATGC GAATGC(1/-1)
BspHI 2486 TCATGA T^CATGA
BsrBI 1907 CCGCTC CCGCTC(-3/-3)
BsrDI 160 GCAATG GCAATG(2/0)
BssSI 381 CACGAG CACGAG(-5/-1)
Bst1107I 2926 GTATAC GTA^TAC
BstEII 222 GGTCACC G^GT[ACGT]ACC
BstPI 222 GGTCACC G^GT[ACGT]ACC
BstZ17I 2926 GTATAC GTA^TAC
CelII 513 GCTGAGC GC^T[ACGT]AGC
Eco47III 172 AGCGCT AGC^GCT
EcoO65I 222 GGTCACC G^GT[ACGT]ACC
EcoRI 2482 GAATTC G^AATTC
KspI 346 CCGCGG CCGC^GG
NcoI 1390 CCATGG C^CATGG
NotI 348 GCGGCCGC GC^GGCCGC
NspI 187 GCATGC [GA]CATG^[CT]
PaeR7I 176 CTCGAG C^TCGAG
PshBI 2613 ATTAAT AT^TAAT
RcaI 2486 TCATGA T^CATGA
SacI 1110 GAGCTC GAGCT^C
SacII 346 CCGCGG CCGC^GG
ScaI 1450 AGTACT AGT^ACT
SpeI 1121 ACTAGT A^CTAGT
SphI 187 GCATGC GCATG^C
XbaI 2519 TCTAGA T^CTAGA
XcmI 2818 CCAGAGGCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 176 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 452 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1056 CAGAAGCTG
AvaI 176 CTCGAG C^[CT]CG[GA]G
218 CTCGGG
BbeI 82 GGCGCC GGCGC^C
137 GGCGCC
BbvCI 908 CCTCAGC CCTCAGC(-5/-2)
2750 CCTCAGC
BciVI 447 GGATAC GTATCC(6/5)
1407 GTATCC
BsoBI 176 CTCGAG C^[CT]CG[GA]G
218 CTCGGG
BspMI 249 ACCTGC ACCTGC(4/8)
1193 GCAGGT
BsrFI 128 GCCGGC [GA]^CCGG[CT]
1875 GCCGGC
BstAPI 715 GCAAAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1193 GCAGGTGTTGC
BstXI 1411 CCATAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2019 CCAACAGATTGG
Cfr10I 128 GCCGGC [GA]^CCGG[CT]
1875 GCCGGC
DsaI 346 CCGCGG C^C[GA][CT]GG
1390 CCATGG
HindIII 532 AAGCTT A^AGCTT
718 AAGCTT
KasI 82 GGCGCC G^GCGCC
137 GGCGCC
MaeII 432 ACGT A^CGT
2111 ACGT
NaeI 128 GCCGGC GCC^GGC
1875 GCCGGC
NarI 82 GGCGCC GG^CGCC
137 GGCGCC
NgoMIV 128 GCCGGC G^CCGGC
1875 GCCGGC
SalI 53 GTCGAC G^TCGAC
1651 GTCGAC
SapI 1161 GAAGAGC GCTCTTC(1/4)
1185 GAAGAGC
SfoI 82 GGCGCC GGC^GCC
137 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AocI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI