##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII Acc65I AhdI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbvCI BcgI BlnI BsaAI
BsiEI BsiWI BsmBI BspDI BssHII
BstXI ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I FseI FspI
KpnI NaeI NdeI NgoMIV NheI
NotI NruI PacI PflFI PmaCI
PmlI PvuI RsrII SalI SfiI
SgrAI SnaBI Tth111I
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 6281 AACGTT AA^CGTT
AcyI 4 GGCGCC G[GA]^CG[CT]C
AgeI 5648 ACCGGT A^CCGGT
AocI 8252 CCTAAGG CC^T[ACGT]AGG
Aor51HI 4421 AGCGCT AGC^GCT
BbeI 4 GGCGCC GGCGC^C
BsaBI 5470 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4 GGCGCC G[GA]^CG[CT]C
BspHI 7924 TCATGA T^CATGA
BsrFI 5648 ACCGGT [GA]^CCGG[CT]
Bst1107I 8382 GTATAC GTA^TAC
BstBI 168 TTCGAA TT^CGAA
BstZ17I 8382 GTATAC GTA^TAC
Bsu36I 8252 CCTAAGG CC^T[ACGT]AGG
Cfr10I 5648 ACCGGT [GA]^CCGG[CT]
Eco47III 4421 AGCGCT AGC^GCT
Eco81I 8252 CCTAAGG CC^T[ACGT]AGG
EcoRI 3638 GAATTC G^AATTC
EcoRV 8374 GATATC GAT^ATC
Hin1I 4 GGCGCC G[GA]^CG[CT]C
HindIII 2401 AAGCTT A^AGCTT
HpaI 4907 GTTAAC GTT^AAC
KasI 4 GGCGCC G^GCGCC
KspI 71 CCGCGG CCGC^GG
MamI 5470 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 18 ACGCGT A^CGCGT
NarI 4 GGCGCC GG^CGCC
NspV 168 TTCGAA TT^CGAA
PaeR7I 4520 CTCGAG C^TCGAG
PinAI 5648 ACCGGT A^CCGGT
PmeI 8999 GTTTAAAC GTTT^AAAC
Psp1406I 6281 AACGTT AA^CGTT
PstI 1985 CTGCAG CTGCA^G
RcaI 7924 TCATGA T^CATGA
SacII 71 CCGCGG CCGC^GG
SapI 660 GAAGAGC GCTCTTC(1/4)
SbfI 1984 CCTGCAGG CCTGCA^GG
ScaI 338 AGTACT AGT^ACT
SexAI 3699 ACCTGGT A^CC[AT]GGT
SfoI 4 GGCGCC GGC^GCC
SfuI 168 TTCGAA TT^CGAA
Sse8387I 1984 CCTGCAGG CCTGCA^GG
StuI 1953 AGGCCT AGG^CCT
SwaI 7553 ATTTAAAT ATTT^AAAT
XbaI 4043 TCTAGA T^CTAGA
XhoI 4520 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 1630 TCCGGA T^CCGGA
2921 TCCGGA
AflII 2768 CTTAAG C^TTAAG
6503 CTTAAG
Alw44I 3667 GTGCAC G^TGCAC
3704 GTGCAC
ApaLI 3667 GTGCAC G^TGCAC
3704 GTGCAC
BclI 3919 TGATCA T^GATCA
8476 TGATCA
BfrI 2768 CTTAAG C^TTAAG
6503 CTTAAG
BlpI 1262 GCTGAGC GC^T[ACGT]AGC
3652 GCTCAGC
Bpu1102I 1262 GCTGAGC GC^T[ACGT]AGC
3652 GCTCAGC
BseAI 1630 TCCGGA T^CCGGA
2921 TCCGGA
BspEI 1630 TCCGGA T^CCGGA
2921 TCCGGA
BstAPI 3276 GCATGCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5956 GCAGATTGTGC
BstEII 653 GGTGACC G^GT[ACGT]ACC
6232 GGTAACC
BstPI 653 GGTGACC G^GT[ACGT]ACC
6232 GGTAACC
CelII 1262 GCTGAGC GC^T[ACGT]AGC
3652 GCTCAGC
DraIII 5483 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
7856 CACCATGTG
EcoO65I 653 GGTGACC G^GT[ACGT]ACC
6232 GGTAACC
FbaI 3919 TGATCA T^GATCA
8476 TGATCA
MfeI 5254 CAATTG C^AATTG
5918 CAATTG
MroI 1630 TCCGGA T^CCGGA
2921 TCCGGA
MunI 5254 CAATTG C^AATTG
5918 CAATTG
NcoI 1011 CCATGG C^CATGG
7281 CCATGG
PshAI 1681 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3886 GACATTTGTC
SacI 2143 GAGCTC GAGCT^C
2530 GAGCTC
SphI 3276 GCATGC GCATG^C
6361 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BciVI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PshBI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SpeI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI