##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AflII AhdI
AocI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbrPI BbsI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BseAI BsiWI BspDI BspEI
BspHI BspLU11I BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NcoI NheI NruI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SalI ScaI SfiI SfuI SnaBI
SphI StuI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AclI 849 AACGTT AA^CGTT
AflIII 2589 ACACGT A^C[GA][CT]GT
AgeI 1651 ACCGGT A^CCGGT
Alw44I 1411 GTGCAC G^TGCAC
Aor51HI 914 AGCGCT AGC^GCT
ApaLI 1411 GTGCAC G^TGCAC
AviII 39 TGCGCA TGC^GCA
BalI 1155 TGGCCA TGG^CCA
BciVI 370 GGATAC GTATCC(6/5)
BsaI 617 GGTCTC GGTCTC(1/5)
BsaWI 1651 ACCGGT [AT]^CCGG[AT]
BsgI 2537 CTGCAC GTGCAG(16/14)
Bsp1407I 3066 TGTACA T^GTACA
BsrGI 3066 TGTACA T^GTACA
BstAPI 1185 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 2776 TTTAAA TTT^AAA
Eco47III 914 AGCGCT AGC^GCT
EcoNI 1555 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2805 ATGCAT ATGCA^T
FspI 39 TGCGCA TGC^GCA
MluNI 1155 TGGCCA TGG^CCA
MscI 1155 TGGCCA TGG^CCA
NsiI 2805 ATGCAT ATGCA^T
PinAI 1651 ACCGGT A^CCGGT
Psp1406I 849 AACGTT AA^CGTT
SacI 2116 GAGCTC GAGCT^C
SexAI 2280 ACCTGGT A^CC[AT]GGT
SpeI 742 ACTAGT A^CTAGT
SspBI 3066 TGTACA T^GTACA
XbaI 791 TCTAGA T^CTAGA
XcmI 1395 CCAAGGAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 547 GTCTAC GT^[AC][GT]AC
584 GTAGAC
AcsI 1307 AAATTC [GA]^AATT[CT]
2879 AAATTT
AlwNI 1186 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1916 CAGAAGCTG
ApoI 1307 AAATTC [GA]^AATT[CT]
2879 AAATTT
AspHI 1411 GTGCAC G[AT]GC[AT]^C
2116 GAGCTC
BbvCI 591 CCTCAGC CCTCAGC(-5/-2)
1210 CCTCAGC
BcgI 1440 GCAGCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1512 GCAGCGCCTTCG
BsiHKAI 1411 GTGCAC G[AT]GC[AT]^C
2116 GAGCTC
BsmBI 3 GAGACG CGTCTC(1/5)
1493 GAGACG
BspMI 458 ACCTGC ACCTGC(4/8)
2483 ACCTGC
BsrDI 2253 GCAATG GCAATG(2/0)
2376 GCAATG
BstYI 794 AGATCC [GA]^GATC[CT]
1402 GGATCT
MflI 794 AGATCC [GA]^GATC[CT]
1402 GGATCT
MslI 1744 CACTTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2798 CATATTGATG
NdeI 2291 CATATG CA^TATG
3070 CATATG
NotI 1640 GCGGCCGC GC^GGCCGC
2150 GCGGCCGC
NspI 830 ACATGC [GA]CATG^[CT]
3016 GCATGT
PaeR7I 1709 CTCGAG C^TCGAG
1931 CTCGAG
PflMI 1147 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1915 CCAGAAGCTGG
SapI 224 GAAGAGC GCTCTTC(1/4)
659 GAAGAGC
SbfI 1555 CCTGCAGG CCTGCA^GG
2484 CCTGCAGG
Sse8387I 1555 CCTGCAGG CCTGCA^GG
2484 CCTGCAGG
SspI 2620 AATATT AAT^ATT
3155 AATATT
TfiI 70 GAATC G^A[AT]TC
339 GATTC
Tsp45I 167 GTCAC ^GT[GC]AC
1648 GTCAC
TspRI 271 CACTG CA[GC]TG[ACGT][ACGT]^
3109 CAGTG
Van91I 1147 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1915 CCAGAAGCTGG
XhoI 1709 CTCGAG C^TCGAG
1931 CTCGAG
XhoII 794 AGATCC [GA]^GATC[CT]
1402 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BanI
BanII BbeI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SgrAI SmaI SmlI
StyI TaqI Tru9I TseI Tsp509I
TthHB8I XmaCI XmaI