##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
BbrPI BbvCI BcgI BclI BfrI
BlpI Bpu1102I BsaAI BsiWI BsmBI
BsmI BspDI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SbfI ScaI SfiI
SfuI SnaBI SpeI Sse8387I SspI
SwaI TfiI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 1212 AAATTC [GA]^AATT[CT]
AhdI 60 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1212 AAATTC [GA]^AATT[CT]
AspEI 60 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2619 CCTAGG C^CTAGG
BbeI 1801 GGCGCC GGCGC^C
BciVI 2975 GTATCC GTATCC(6/5)
BglI 2756 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2191 AGATCT A^GATCT
BlnI 2619 CCTAGG C^CTAGG
Bsp1407I 3217 TGTACA T^GTACA
BspHI 2152 TCATGA T^CATGA
BspLU11I 2341 ACATGT A^CATGT
BsrDI 506 CATTGC GCAATG(2/0)
BsrGI 3217 TGTACA T^GTACA
BstEII 1562 GGTGACC G^GT[ACGT]ACC
BstPI 1562 GGTGACC G^GT[ACGT]ACC
DraI 2510 TTTAAA TTT^AAA
Eam1105I 60 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1562 GGTGACC G^GT[ACGT]ACC
HaeII 1801 GGCGCC [GA]GCGC^[CT]
KasI 1801 GGCGCC G^GCGCC
NarI 1801 GGCGCC GG^CGCC
PaeR7I 2922 CTCGAG C^TCGAG
RcaI 2152 TCATGA T^CATGA
SalI 68 GTCGAC G^TCGAC
SfoI 1801 GGCGCC GGC^GCC
SgrAI 1803 CGCCGGCG C[GA]^CCGG[CT]G
SphI 470 GCATGC GCATG^C
SspBI 3217 TGTACA T^GTACA
StuI 1594 AGGCCT AGG^CCT
XhoI 2922 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 579 GACGTC GACGT^C
1242 GACGTC
AccI 68 GTCGAC GT^[AC][GT]AC
1713 GTCTAC
AccII 369 CGCG CG^CG
1398 CGCG
AccIII 91 TCCGGA T^CCGGA
734 TCCGGA
AflIII 1531 ACGTGT A^C[GA][CT]GT
2341 ACATGT
AocI 2479 CCTGAGG CC^T[ACGT]AGG
2789 CCTAAGG
AspHI 557 GAGCAC G[AT]GC[AT]^C
921 GTGCTC
BamHI 856 GGATCC G^GATCC
3027 GGATCC
BsaBI 1005 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2192 GATCTGCATC
BseAI 91 TCCGGA T^CCGGA
734 TCCGGA
BsiEI 70 CGACCG CG[GA][CT]^CG
2064 CGACCG
BsiHKAI 557 GAGCAC G[AT]GC[AT]^C
921 GTGCTC
BspEI 91 TCCGGA T^CCGGA
734 TCCGGA
BstUI 369 CGCG CG^CG
1398 CGCG
Bsu36I 2479 CCTGAGG CC^T[ACGT]AGG
2789 CCTAAGG
CfoI 1733 GCGC GCG^C
1802 GCGC
DraIII 263 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
824 CACCGTGTG
Eco81I 2479 CCTGAGG CC^T[ACGT]AGG
2789 CCTAAGG
HgaI 122 GACGC GACGC(5/10)
2350 GCGTC
HhaI 1733 GCGC GCG^C
1802 GCGC
HinP1I 1733 GCGC G^CGC
1802 GCGC
HincII 68 GTCGAC GT[CT]^[GA]AC
1527 GTCAAC
HindII 68 GTCGAC GT[CT]^[GA]AC
1527 GTCAAC
MamI 1005 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2192 GATCTGCATC
MroI 91 TCCGGA T^CCGGA
734 TCCGGA
MseI 2511 TTAA T^TAA
2701 TTAA
MvnI 369 CGCG CG^CG
1398 CGCG
SmaI 429 CCCGGG CCC^GGG
1288 CCCGGG
Tru9I 2511 TTAA T^TAA
2701 TTAA
Tsp509I 1213 AATT ^AATT
2514 AATT
XmaCI 429 CCCGGG C^CCGGG
1288 CCCGGG
XmaI 429 CCCGGG C^CCGGG
1288 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII Hin1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII