##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
BbrPI       BbvCI       BcgI        BclI        BfrI        
BlpI        Bpu1102I    BsaAI       BsiWI       BsmBI       
BsmI        BspDI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     CelII       ClaI        
CpoI        EagI        EclXI       Eco47III    Eco52I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KpnI        KspI        
MfeI        MluI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SbfI        ScaI        SfiI        
SfuI        SnaBI       SpeI        Sse8387I    SspI        
SwaI        TfiI        Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AcsI         1212  AAATTC               [GA]^AATT[CT]
AhdI           60  GACATGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         1212  AAATTC               [GA]^AATT[CT]
AspEI          60  GACATGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        2619  CCTAGG               C^CTAGG
BbeI         1801  GGCGCC               GGCGC^C
BciVI        2975  GTATCC               GTATCC(6/5)
BglI         2756  GCCCCAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2191  AGATCT               A^GATCT
BlnI         2619  CCTAGG               C^CTAGG
Bsp1407I     3217  TGTACA               T^GTACA
BspHI        2152  TCATGA               T^CATGA
BspLU11I     2341  ACATGT               A^CATGT
BsrDI         506  CATTGC               GCAATG(2/0)
BsrGI        3217  TGTACA               T^GTACA
BstEII       1562  GGTGACC              G^GT[ACGT]ACC
BstPI        1562  GGTGACC              G^GT[ACGT]ACC
DraI         2510  TTTAAA               TTT^AAA
Eam1105I       60  GACATGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1562  GGTGACC              G^GT[ACGT]ACC
HaeII        1801  GGCGCC               [GA]GCGC^[CT]
KasI         1801  GGCGCC               G^GCGCC
NarI         1801  GGCGCC               GG^CGCC
PaeR7I       2922  CTCGAG               C^TCGAG
RcaI         2152  TCATGA               T^CATGA
SalI           68  GTCGAC               G^TCGAC
SfoI         1801  GGCGCC               GGC^GCC
SgrAI        1803  CGCCGGCG             C[GA]^CCGG[CT]G
SphI          470  GCATGC               GCATG^C
SspBI        3217  TGTACA               T^GTACA
StuI         1594  AGGCCT               AGG^CCT
XhoI         2922  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AatII         579  GACGTC               GACGT^C
             1242  GACGTC              
AccI           68  GTCGAC               GT^[AC][GT]AC
             1713  GTCTAC              
AccII         369  CGCG                 CG^CG
             1398  CGCG                
AccIII         91  TCCGGA               T^CCGGA
              734  TCCGGA              
AflIII       1531  ACGTGT               A^C[GA][CT]GT
             2341  ACATGT              
AocI         2479  CCTGAGG              CC^T[ACGT]AGG
             2789  CCTAAGG             
AspHI         557  GAGCAC               G[AT]GC[AT]^C
              921  GTGCTC              
BamHI         856  GGATCC               G^GATCC
             3027  GGATCC              
BsaBI        1005  GATGCCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2192  GATCTGCATC          
BseAI          91  TCCGGA               T^CCGGA
              734  TCCGGA              
BsiEI          70  CGACCG               CG[GA][CT]^CG
             2064  CGACCG              
BsiHKAI       557  GAGCAC               G[AT]GC[AT]^C
              921  GTGCTC              
BspEI          91  TCCGGA               T^CCGGA
              734  TCCGGA              
BstUI         369  CGCG                 CG^CG
             1398  CGCG                
Bsu36I       2479  CCTGAGG              CC^T[ACGT]AGG
             2789  CCTAAGG             
CfoI         1733  GCGC                 GCG^C
             1802  GCGC                
DraIII        263  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              824  CACCGTGTG           
Eco81I       2479  CCTGAGG              CC^T[ACGT]AGG
             2789  CCTAAGG             
HgaI          122  GACGC                GACGC(5/10)
             2350  GCGTC               
HhaI         1733  GCGC                 GCG^C
             1802  GCGC                
HinP1I       1733  GCGC                 G^CGC
             1802  GCGC                
HincII         68  GTCGAC               GT[CT]^[GA]AC
             1527  GTCAAC              
HindII         68  GTCGAC               GT[CT]^[GA]AC
             1527  GTCAAC              
MamI         1005  GATGCCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2192  GATCTGCATC          
MroI           91  TCCGGA               T^CCGGA
              734  TCCGGA              
MseI         2511  TTAA                 T^TAA
             2701  TTAA                
MvnI          369  CGCG                 CG^CG
             1398  CGCG                
SmaI          429  CCCGGG               CCC^GGG
             1288  CCCGGG              
Tru9I        2511  TTAA                 T^TAA
             2701  TTAA                
Tsp509I      1213  AATT                 ^AATT
             2514  AATT                
XmaCI         429  CCCGGG               C^CCGGG
             1288  CCCGGG              
XmaI          429  CCCGGG               C^CCGGG
             1288  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DrdI        DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       Hin1I       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmlI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XcmI        XhoII