################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## Acc65I AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII BbrPI BbvCI BcgI BclI BfrI BlpI Bpu1102I BsaAI BsiWI BsmBI BsmI BspDI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SbfI ScaI SfiI SfuI SnaBI SpeI Sse8387I SspI SwaI TfiI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AcsI 1212 AAATTC [GA]^AATT[CT] AhdI 60 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 1212 AAATTC [GA]^AATT[CT] AspEI 60 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 2619 CCTAGG C^CTAGG BbeI 1801 GGCGCC GGCGC^C BciVI 2975 GTATCC GTATCC(6/5) BglI 2756 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2191 AGATCT A^GATCT BlnI 2619 CCTAGG C^CTAGG Bsp1407I 3217 TGTACA T^GTACA BspHI 2152 TCATGA T^CATGA BspLU11I 2341 ACATGT A^CATGT BsrDI 506 CATTGC GCAATG(2/0) BsrGI 3217 TGTACA T^GTACA BstEII 1562 GGTGACC G^GT[ACGT]ACC BstPI 1562 GGTGACC G^GT[ACGT]ACC DraI 2510 TTTAAA TTT^AAA Eam1105I 60 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1562 GGTGACC G^GT[ACGT]ACC HaeII 1801 GGCGCC [GA]GCGC^[CT] KasI 1801 GGCGCC G^GCGCC NarI 1801 GGCGCC GG^CGCC PaeR7I 2922 CTCGAG C^TCGAG RcaI 2152 TCATGA T^CATGA SalI 68 GTCGAC G^TCGAC SfoI 1801 GGCGCC GGC^GCC SgrAI 1803 CGCCGGCG C[GA]^CCGG[CT]G SphI 470 GCATGC GCATG^C SspBI 3217 TGTACA T^GTACA StuI 1594 AGGCCT AGG^CCT XhoI 2922 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 579 GACGTC GACGT^C 1242 GACGTC AccI 68 GTCGAC GT^[AC][GT]AC 1713 GTCTAC AccII 369 CGCG CG^CG 1398 CGCG AccIII 91 TCCGGA T^CCGGA 734 TCCGGA AflIII 1531 ACGTGT A^C[GA][CT]GT 2341 ACATGT AocI 2479 CCTGAGG CC^T[ACGT]AGG 2789 CCTAAGG AspHI 557 GAGCAC G[AT]GC[AT]^C 921 GTGCTC BamHI 856 GGATCC G^GATCC 3027 GGATCC BsaBI 1005 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2192 GATCTGCATC BseAI 91 TCCGGA T^CCGGA 734 TCCGGA BsiEI 70 CGACCG CG[GA][CT]^CG 2064 CGACCG BsiHKAI 557 GAGCAC G[AT]GC[AT]^C 921 GTGCTC BspEI 91 TCCGGA T^CCGGA 734 TCCGGA BstUI 369 CGCG CG^CG 1398 CGCG Bsu36I 2479 CCTGAGG CC^T[ACGT]AGG 2789 CCTAAGG CfoI 1733 GCGC GCG^C 1802 GCGC DraIII 263 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 824 CACCGTGTG Eco81I 2479 CCTGAGG CC^T[ACGT]AGG 2789 CCTAAGG HgaI 122 GACGC GACGC(5/10) 2350 GCGTC HhaI 1733 GCGC GCG^C 1802 GCGC HinP1I 1733 GCGC G^CGC 1802 GCGC HincII 68 GTCGAC GT[CT]^[GA]AC 1527 GTCAAC HindII 68 GTCGAC GT[CT]^[GA]AC 1527 GTCAAC MamI 1005 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2192 GATCTGCATC MroI 91 TCCGGA T^CCGGA 734 TCCGGA MseI 2511 TTAA T^TAA 2701 TTAA MvnI 369 CGCG CG^CG 1398 CGCG SmaI 429 CCCGGG CCC^GGG 1288 CCCGGG Tru9I 2511 TTAA T^TAA 2701 TTAA Tsp509I 1213 AATT ^AATT 2514 AATT XmaCI 429 CCCGGG C^CCGGG 1288 CCCGGG XmaI 429 CCCGGG C^CCGGG 1288 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII Hin1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII