##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I AviII AvrII BbrPI BciVI
BclI BfrI BglII BlnI BsaBI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
BstUI Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MluI
MroI MvnI NaeI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SgrAI SmaI SpeI
SphI Sse8387I SwaI Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 297 GACGTC GACGT^C
AhdI 587 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3218 GTGCAC G^TGCAC
AlwNI 89 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 109 CCTCAGG CC^T[ACGT]AGG
ApaI 879 GGGCCC GGGCC^C
ApaLI 3218 GTGCAC G^TGCAC
AspEI 587 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3145 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1005 GGATCC G^GATCC
BbeI 54 GGCGCC GGCGC^C
BbsI 960 GTCTTC GAAGAC(2/6)
BcgI 1494 GCACACAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 3006 CCGGG CC^[GC]GG
BglI 1826 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 960 GTCTTC GAAGAC(2/6)
BsaAI 2630 TACGTA [CT]AC^GT[GA]
BsaWI 642 ACCGGA [AT]^CCGG[AT]
BsmBI 3150 CGTCTC CGTCTC(1/5)
BsmI 2671 GCATTC GAATGC(1/-1)
BsrDI 1791 GCAATG GCAATG(2/0)
Bst1107I 568 GTATAC GTA^TAC
BstAPI 1118 GCATGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2948 TTCGAA TT^CGAA
BstEII 1747 GGTAACC G^GT[ACGT]ACC
BstPI 1747 GGTAACC G^GT[ACGT]ACC
BstZ17I 568 GTATAC GTA^TAC
Bsu36I 109 CCTCAGG CC^T[ACGT]AGG
Eam1105I 587 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 696 CTCTTC CTCTTC(1/4)
Eco81I 109 CCTCAGG CC^T[ACGT]AGG
EcoNI 61 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1747 GGTAACC G^GT[ACGT]ACC
EcoRI 100 GAATTC G^AATTC
EcoT22I 1887 ATGCAT ATGCA^T
FauI 2843 GCGGG CCCGC(4/6)
HaeII 54 GGCGCC [GA]GCGC^[CT]
KasI 54 GGCGCC G^GCGCC
Ksp632I 696 CTCTTC CTCTTC(1/4)
NarI 54 GGCGCC GG^CGCC
NciI 3006 CCGGG CC^[GC]GG
NsiI 1887 ATGCAT ATGCA^T
NspV 2948 TTCGAA TT^CGAA
PflFI 3145 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 2025 CTGCAG CTGCA^G
SacI 450 GAGCTC GAGCT^C
SexAI 1628 ACCAGGT A^CC[AT]GGT
SfcI 2025 CTGCAG C^T[GA][CT]AG
SfoI 54 GGCGCC GGC^GCC
SfuI 2948 TTCGAA TT^CGAA
SnaBI 2630 TACGTA TAC^GTA
SspI 1549 AATATT AAT^ATT
StuI 778 AGGCCT AGG^CCT
Tth111I 3145 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 712 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 568 GTATAC GT^[AC][GT]AC
2484 GTCTAC
Asp700I 100 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1132 GAATTGGTTC
BanI 54 GGCGCC G^G[CT][GA]CC
1256 GGTGCC
BanII 450 GAGCTC G[GA]GC[CT]^C
879 GGGCCC
BbvCI 1777 CCTCAGC CCTCAGC(-5/-2)
3054 GCTGAGG
Bsp1407I 14 TGTACA T^GTACA
3197 TGTACA
BsrGI 14 TGTACA T^GTACA
3197 TGTACA
BstXI 668 CCAAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2903 CCAGCACTTTGG
BstYI 528 AGATCC [GA]^GATC[CT]
1005 GGATCC
HapII 643 CCGG C^CGG
3006 CCGG
HgaI 2784 GACGC GACGC(5/10)
2482 GCGTC
HincII 973 GTTGAC GT[CT]^[GA]AC
2595 GTCAAC
HindII 973 GTTGAC GT[CT]^[GA]AC
2595 GTCAAC
HpaII 643 CCGG C^CGG
3006 CCGG
MaeII 298 ACGT A^CGT
2631 ACGT
MfeI 1852 CAATTG C^AATTG
2557 CAATTG
MflI 528 AGATCC [GA]^GATC[CT]
1005 GGATCC
MspI 643 CCGG C^CGG
3006 CCGG
MunI 1852 CAATTG C^AATTG
2557 CAATTG
PpuMI 221 AGGTCCC [GA]G^G[AT]CC[CT]
1434 AGGACCC
SmlI 1010 CTCAAG C^T[CT][GA]AG
1229 CTCAAG
SspBI 14 TGTACA T^GTACA
3197 TGTACA
TfiI 351 GAATC G^A[AT]TC
1326 GAATC
XhoII 528 AGATCC [GA]^GATC[CT]
1005 GGATCC
XmnI 100 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1132 GAATTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BalI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI Hin1I HinP1I
HinfI HphI ItaI MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I