##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AvrII
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BsaAI BsgI BsiWI
BspDI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoT22I
FseI HpaI KpnI MfeI MluI
MunI NdeI NheI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 502 TCCGGA T^CCGGA
AgeI 708 ACCGGT A^CCGGT
AviII 1150 TGCGCA TGC^GCA
BalI 378 TGGCCA TGG^CCA
BamHI 2695 GGATCC G^GATCC
BbeI 44 GGCGCC GGCGC^C
BclI 405 TGATCA T^GATCA
BsaBI 2696 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2537 GAGACC GGTCTC(1/5)
BseAI 502 TCCGGA T^CCGGA
BsmBI 522 GAGACG CGTCTC(1/5)
Bsp1407I 1002 TGTACA T^GTACA
BspEI 502 TCCGGA T^CCGGA
BsrGI 1002 TGTACA T^GTACA
BstAPI 811 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1027 GGTCACC G^GT[ACGT]ACC
BstPI 1027 GGTCACC G^GT[ACGT]ACC
DraIII 2309 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 52 CGGCCG C^GGCCG
EclXI 52 CGGCCG C^GGCCG
Eco52I 52 CGGCCG C^GGCCG
EcoO65I 1027 GGTCACC G^GT[ACGT]ACC
FbaI 405 TGATCA T^GATCA
FspI 1150 TGCGCA TGC^GCA
HincII 2044 GTCAAC GT[CT]^[GA]AC
HindII 2044 GTCAAC GT[CT]^[GA]AC
KasI 44 GGCGCC G^GCGCC
MamI 2696 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 378 TGGCCA TGG^CCA
MroI 502 TCCGGA T^CCGGA
MscI 378 TGGCCA TGG^CCA
MslI 2577 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1717 GCCGGC GCC^GGC
NarI 44 GGCGCC GG^CGCC
NcoI 2661 CCATGG C^CATGG
NgoMIV 1717 GCCGGC G^CCGGC
NotI 51 GCGGCCGC GC^GGCCGC
PinAI 708 ACCGGT A^CCGGT
SbfI 868 CCTGCAGG CCTGCA^GG
SfoI 44 GGCGCC GGC^GCC
SmaI 133 CCCGGG CCC^GGG
SmlI 1491 CTCAAG C^T[CT][GA]AG
Sse8387I 868 CCTGCAGG CCTGCA^GG
SspBI 1002 TGTACA T^GTACA
XmaCI 133 CCCGGG C^CCGGG
XmaI 133 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 2027 GACGTC GACGT^C
2805 GACGTC
ApaI 136 GGGCCC GGGCC^C
2683 GGGCCC
AspI 2234 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2435 GACCTTGTC
AvaI 133 CCCGGG C^[CT]CG[GA]G
686 CCCGAG
BglII 2484 AGATCT A^GATCT
3068 AGATCT
BsiEI 52 CGGCCG CG[GA][CT]^CG
84 CGACCG
BsmI 2498 GAATGC GAATGC(1/-1)
1742 GCATTC
BsoBI 133 CCCGGG C^[CT]CG[GA]G
686 CCCGAG
BspHI 2768 TCATGA T^CATGA
3020 TCATGA
BsrFI 708 ACCGGT [GA]^CCGG[CT]
1717 GCCGGC
Cfr10I 708 ACCGGT [GA]^CCGG[CT]
1717 GCCGGC
EcoRI 1569 GAATTC G^AATTC
3115 GAATTC
HaeII 10 AGCGCC [GA]GCGC^[CT]
44 GGCGCC
MaeII 2028 ACGT A^CGT
2806 ACGT
PflFI 2234 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2435 GACCTTGTC
PflMI 1289 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2661 CCATGGGTTGG
RcaI 2768 TCATGA T^CATGA
3020 TCATGA
SexAI 1408 ACCAGGT A^CC[AT]GGT
2381 ACCTGGT
TaqI 520 TCGA T^CGA
3001 TCGA
Tth111I 2234 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2435 GACCTTGTC
TthHB8I 520 TCGA T^CGA
3001 TCGA
Van91I 1289 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2661 CCATGGGTTGG
XcmI 599 CCACCATCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1427 CCAGGTTGGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI