##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AcyI AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AspEI
AspI AviII BalI BbeI BbrPI
BcgI BclI BglI BlpI Bpu1102I
BsaAI BsaHI BsaI BsiEI BsmBI
Bsp1407I BspDI BspLU11I BsrDI BsrFI
BsrGI BstAPI BstBI BstEII BstPI
BstXI CelII Cfr10I ClaI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KspI
MluI MluNI MscI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 194 GGTACC G^GTACC
AccI 2423 GTATAC GT^[AC][GT]AC
AccIII 348 TCCGGA T^CCGGA
AclI 1233 AACGTT AA^CGTT
AflII 1521 CTTAAG C^TTAAG
AlwNI 1046 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1886 CCTGAGG CC^T[ACGT]AGG
AseI 777 ATTAAT AT^TAAT
AsnI 777 ATTAAT AT^TAAT
Asp718I 194 GGTACC G^GTACC
AvaI 2137 CCCGGG C^[CT]CG[GA]G
BamHI 1260 GGATCC G^GATCC
BbsI 388 GTCTTC GAAGAC(2/6)
BciVI 2602 GTATCC GTATCC(6/5)
BfrI 1521 CTTAAG C^TTAAG
BglII 1407 AGATCT A^GATCT
BpuAI 388 GTCTTC GAAGAC(2/6)
BsaBI 1813 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 348 TCCGGA [AT]^CCGG[AT]
BseAI 348 TCCGGA T^CCGGA
BsgI 253 GTGCAG GTGCAG(16/14)
BsiWI 339 CGTACG C^GTACG
BsmAI 1210 GAGAC GTCTC(1/5)
BsoBI 2137 CCCGGG C^[CT]CG[GA]G
BspEI 348 TCCGGA T^CCGGA
BspHI 157 TCATGA T^CATGA
BspMI 255 GCAGGT ACCTGC(4/8)
BsrBI 121 CCGCTC CCGCTC(-3/-3)
BssHII 131 GCGCGC G^CGCGC
BssSI 243 CTCGTG CACGAG(-5/-1)
Bst1107I 2423 GTATAC GTA^TAC
BstZ17I 2423 GTATAC GTA^TAC
Bsu36I 1886 CCTGAGG CC^T[ACGT]AGG
CpoI 345 CGGTCCG CG^G[AT]CCG
DraIII 370 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1886 CCTGAGG CC^T[ACGT]AGG
EcoRI 2837 GAATTC G^AATTC
FauI 82 GCGGG CCCGC(4/6)
HindIII 405 AAGCTT A^AGCTT
KpnI 194 GGTACC GGTAC^C
MaeII 1234 ACGT A^CGT
MamI 1813 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 485 CAATTG C^AATTG
MroI 348 TCCGGA T^CCGGA
MunI 485 CAATTG C^AATTG
NcoI 2624 CCATGG C^CATGG
NheI 238 GCTAGC G^CTAGC
NspI 3231 GCATGC [GA]CATG^[CT]
PflMI 2589 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 777 ATTAAT AT^TAAT
Psp1406I 1233 AACGTT AA^CGTT
PstI 1750 CTGCAG CTGCA^G
RcaI 157 TCATGA T^CATGA
RsrII 345 CGGTCCG CG^G[AT]CCG
ScaI 2355 AGTACT AGT^ACT
SmaI 2137 CCCGGG CCC^GGG
SmlI 1521 CTTAAG C^T[CT][GA]AG
SphI 3231 GCATGC GCATG^C
StuI 1480 AGGCCT AGG^CCT
Van91I 2589 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2137 CCCGGG C^CCGGG
XmaI 2137 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 223 GGGCCC GGGCC^C
2585 GGGCCC
Asp700I 403 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3136 GAAGGATTTC
AspHI 352 GAGCAC G[AT]GC[AT]^C
787 GAGCAC
AvrII 412 CCTAGG C^CTAGG
517 CCTAGG
BanI 194 GGTACC G^G[CT][GA]CC
275 GGCACC
BanII 223 GGGCCC G[GA]GC[CT]^C
2585 GGGCCC
BbvCI 2104 CCTCAGC CCTCAGC(-5/-2)
169 GCTGAGG
BlnI 412 CCTAGG C^CTAGG
517 CCTAGG
BsiHKAI 352 GAGCAC G[AT]GC[AT]^C
787 GAGCAC
EcoT22I 1906 ATGCAT ATGCA^T
2256 ATGCAT
NdeI 2652 CATATG CA^TATG
2988 CATATG
NsiI 1906 ATGCAT ATGCA^T
2256 ATGCAT
PleI 811 GACTC GAGTC(4/5)
1465 GACTC
PpuMI 653 GGGACCC [GA]G^G[AT]CC[CT]
1383 AGGTCCT
PvuII 167 CAGCTG CAG^CTG
2924 CAGCTG
SfcI 1750 CTGCAG C^T[GA][CT]AG
1855 CTACAG
SspI 2780 AATATT AAT^ATT
3126 AATATT
Tsp45I 1679 GTGAC ^GT[GC]AC
2411 GTGAC
XmnI 403 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3136 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII